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Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HP...

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Detalles Bibliográficos
Autores principales: Kaewbai-Ngam, Janine, Incharoensakdi, Aran, Monshupanee, Tanakarn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450728/
https://www.ncbi.nlm.nih.gov/pubmed/37638303
http://dx.doi.org/10.21769/BioProtoc.4790