Cargando…

Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HP...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kaewbai-Ngam, Janine, Incharoensakdi, Aran, Monshupanee, Tanakarn
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2023
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450728/ https://www.ncbi.nlm.nih.gov/pubmed/37638303 http://dx.doi.org/10.21769/BioProtoc.4790

Ejemplares similares

Two-stage (photoautotrophy and heterotrophy) cultivation enables efficient production of bioplastic poly-3-hydroxybutyrate in auto-sedimenting cyanobacterium
por: Monshupanee, Tanakarn, et al.
Publicado: (2016)

Utilization of shrimp wastewater for poly-β-hydroxybutyrate production by Synechocystis sp. PCC 6803 strain ΔSphU cultivated in photobioreactor
por: Krasaesueb, Nattawut, et al.
Publicado: (2019)

Disruption of cyanobacterial γ-aminobutyric acid shunt pathway reduces metabolites levels in tricarboxylic acid cycle, but enhances pyruvate and poly(3-hydroxybutyrate) accumulation
por: Monshupanee, Tanakarn, et al.
Publicado: (2019)

Genomic Responses to Arsenic in the Cyanobacterium Synechocystis sp. PCC 6803
por: Sánchez-Riego, Ana María, et al.
Publicado: (2014)

Genomic Structure of the Cyanobacterium Synechocystis sp. PCC 6803 Strain GT-S
por: Tajima, Naoyuki, et al.
Publicado: (2011)

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803
por: Hernández-Prieto, Miguel A., et al.
Publicado: (2016)

Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
por: Pade, Nadin, et al.
Publicado: (2016)

Glutaredoxins are essential for stress adaptation in the cyanobacterium Synechocystis sp. PCC 6803
por: Sánchez-Riego, Ana M., et al.
Publicado: (2013)

Functional Diversity of Transcriptional Regulators in the Cyanobacterium Synechocystis sp. PCC 6803
por: Shi, Mengliang, et al.
Publicado: (2017)

An Improved Natural Transformation Protocol for the Cyanobacterium Synechocystis sp. PCC 6803
por: Pope, Matthew A., et al.
Publicado: (2020)

On the Role and Production of Polyhydroxybutyrate (PHB) in the Cyanobacterium Synechocystis sp. PCC 6803
por: Koch, Moritz, et al.
Publicado: (2020)

Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803
por: Kugler, Amit, et al.
Publicado: (2023)

Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714
por: Kamravamanesh, Donya, et al.
Publicado: (2017)

Metal Oxide Mediated Extracellular NADPH Regeneration Improves Ethanol Production by Engineered Synechocystis sp. PCC 6803
por: Velmurugan, Rajendran, et al.
Publicado: (2019)

Advances in the Function and Regulation of Hydrogenase in the Cyanobacterium Synechocystis PCC6803
por: Cassier-Chauvat, Corinne, et al.
Publicado: (2014)

Cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene produces higher polyhydroxybutyrate accumulation under modified nutrients of acetate supplementation and nitrogen-phosphorus starvation
por: Utharn, Suthira, et al.
Publicado: (2021)

Refolding and Enzyme Kinetic Studies on the Ferrochelatase of the Cyanobacterium Synechocystis sp. PCC 6803
por: Storm, Patrik, et al.
Publicado: (2013)

Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803
por: Giner-Lamia, Joaquin, et al.
Publicado: (2014)

Transcriptomic response to prolonged ethanol production in the cyanobacterium Synechocystis sp. PCC6803
por: Dienst, Dennis, et al.
Publicado: (2014)

Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803
por: Hagemann, Martin, et al.
Publicado: (2018)

Manganese acquisition is facilitated by PilA in the cyanobacterium Synechocystis sp. PCC 6803
por: Lamb, Jacob J., et al.
Publicado: (2017)

Regulation of the scp Genes in the Cyanobacterium Synechocystis sp. PCC 6803—What is New?
por: Cheregi, Otilia, et al.
Publicado: (2015)

Characterization of Lysine Monomethylome and Methyltransferase in Model Cyanobacterium Synechocystis sp. PCC 6803
por: Lin, Xiaohuang, et al.
Publicado: (2020)

Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium Synechocystis sp. PCC 6803
por: Mager, Maurice, et al.
Publicado: (2023)

Function and Regulation of Ferredoxins in the Cyanobacterium, Synechocystis PCC6803: Recent Advances
por: Cassier-Chauvat, Corinne, et al.
Publicado: (2014)

Na(+)-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter
por: Burut-Archanai, Surachet, et al.
Publicado: (2011)

The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
por: Morrison, S Shawn, et al.
Publicado: (2005)

Functional Role of PilA in Iron Acquisition in the Cyanobacterium Synechocystis sp. PCC 6803
por: Lamb, Jacob J., et al.
Publicado: (2014)

Phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation
por: Spät, Philipp, et al.
Publicado: (2015)

Type II Toxin–Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803
por: Kopfmann, Stefan, et al.
Publicado: (2016)

The structure and reactivity of the HoxEFU complex from the cyanobacterium Synechocystis sp. PCC 6803
por: Artz, Jacob H., et al.
Publicado: (2020)

Type IV Pili-Independent Photocurrent Production by the Cyanobacterium Synechocystis sp. PCC 6803
por: Thirumurthy, Miyuki A., et al.
Publicado: (2020)

Factors Controlling Floc Formation and Structure in the Cyanobacterium Synechocystis sp. Strain PCC 6803
por: Conradi, Fabian D., et al.
Publicado: (2019)

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
por: Yao, Lun, et al.
Publicado: (2020)

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803
por: Chen, Zhuo, et al.
Publicado: (2020)

Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803
por: Brandenburg, Fabian, et al.
Publicado: (2020)

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803
por: Durall, Claudia, et al.
Publicado: (2020)

Protein Network Signatures Associated with Exogenous Biofuels Treatments in Cyanobacterium Synechocystis sp. PCC 6803
por: Pei, Guangsheng, et al.
Publicado: (2014)

[NiFe]-hydrogenase is essential for cyanobacterium Synechocystis sp. PCC 6803 aerobic growth in the dark
por: De Rosa, Edith, et al.
Publicado: (2015)

The ω Subunit of RNA Polymerase Is Essential for Thermal Acclimation of the Cyanobacterium Synechocystis Sp. PCC 6803
por: Gunnelius, Liisa, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_o8bek599oess65m0lpilenvpu0