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Real-Time Monitoring of the Level and Activity of Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR
[Image: see text] Visualization and quantification of important biomolecules like glutathione (GSH) in live cells are highly important. The existing methods are mostly from optical detection and lack of atomic resolution on the activity of GSH. Here, we present a sensitive (19)F-NMR method to quanti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451033/ https://www.ncbi.nlm.nih.gov/pubmed/37637729 http://dx.doi.org/10.1021/acscentsci.3c00385 |
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author | Cui, Chao-Yu Li, Bin Su, Xun-Cheng |
author_facet | Cui, Chao-Yu Li, Bin Su, Xun-Cheng |
author_sort | Cui, Chao-Yu |
collection | PubMed |
description | [Image: see text] Visualization and quantification of important biomolecules like glutathione (GSH) in live cells are highly important. The existing methods are mostly from optical detection and lack of atomic resolution on the activity of GSH. Here, we present a sensitive (19)F-NMR method to quantify real-time variations of GSH in live cells in a reversible manner. This NMR method prevents extracellular leakage and irreversible consumption of intracellular GSH during the detection. The high performance of the reactive (19)F-probe enables accurate determination of intracellular GSH content at atomic resolution, from which information on GSH variations with respect to the extracellular and intracellular conditions can be inferred. In addition, we demonstrate the applicability of this NMR method to quantify the GSH levels between different live cell lines and to disclose the distinct differences between the intracellular environment and cell lysates. We foresee the application of (19)F-NMR to monitor real-time variations of intracellular GSH levels in relation to GSH-involved central cellular processes. |
format | Online Article Text |
id | pubmed-10451033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104510332023-08-26 Real-Time Monitoring of the Level and Activity of Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR Cui, Chao-Yu Li, Bin Su, Xun-Cheng ACS Cent Sci [Image: see text] Visualization and quantification of important biomolecules like glutathione (GSH) in live cells are highly important. The existing methods are mostly from optical detection and lack of atomic resolution on the activity of GSH. Here, we present a sensitive (19)F-NMR method to quantify real-time variations of GSH in live cells in a reversible manner. This NMR method prevents extracellular leakage and irreversible consumption of intracellular GSH during the detection. The high performance of the reactive (19)F-probe enables accurate determination of intracellular GSH content at atomic resolution, from which information on GSH variations with respect to the extracellular and intracellular conditions can be inferred. In addition, we demonstrate the applicability of this NMR method to quantify the GSH levels between different live cell lines and to disclose the distinct differences between the intracellular environment and cell lysates. We foresee the application of (19)F-NMR to monitor real-time variations of intracellular GSH levels in relation to GSH-involved central cellular processes. American Chemical Society 2023-07-21 /pmc/articles/PMC10451033/ /pubmed/37637729 http://dx.doi.org/10.1021/acscentsci.3c00385 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Cui, Chao-Yu Li, Bin Su, Xun-Cheng Real-Time Monitoring of the Level and Activity of Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title | Real-Time Monitoring
of the Level and Activity of
Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title_full | Real-Time Monitoring
of the Level and Activity of
Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title_fullStr | Real-Time Monitoring
of the Level and Activity of
Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title_full_unstemmed | Real-Time Monitoring
of the Level and Activity of
Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title_short | Real-Time Monitoring
of the Level and Activity of
Intracellular Glutathione in Live Cells at Atomic Resolution by (19)F-NMR |
title_sort | real-time monitoring
of the level and activity of
intracellular glutathione in live cells at atomic resolution by (19)f-nmr |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451033/ https://www.ncbi.nlm.nih.gov/pubmed/37637729 http://dx.doi.org/10.1021/acscentsci.3c00385 |
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