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Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology
SIMPLE SUMMARY: Estimation of age represents a challenge in the veterinary forensic pathology field as well as in human forensic medicine. In puppies, the most common methods of age estimation are the visual examination of the dentition and the skeletal age. Nevertheless, these methods are affected...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451229/ https://www.ncbi.nlm.nih.gov/pubmed/37627455 http://dx.doi.org/10.3390/ani13162665 |
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author | d’Aquino, Ilaria Piegari, Giuseppe Miletti, Gianluca Sannino, Emanuela Costanza, Dario Meomartino, Leonardo Fico, Rosario Riccio, Lorenzo Vaccaro, Emanuela De Biase, Davide Paciello, Orlando |
author_facet | d’Aquino, Ilaria Piegari, Giuseppe Miletti, Gianluca Sannino, Emanuela Costanza, Dario Meomartino, Leonardo Fico, Rosario Riccio, Lorenzo Vaccaro, Emanuela De Biase, Davide Paciello, Orlando |
author_sort | d’Aquino, Ilaria |
collection | PubMed |
description | SIMPLE SUMMARY: Estimation of age represents a challenge in the veterinary forensic pathology field as well as in human forensic medicine. In puppies, the most common methods of age estimation are the visual examination of the dentition and the skeletal age. Nevertheless, these methods are affected by a broad range of variables. In contrast, the kidney is characterized by a precise postnatal development. In human glomerulogenesis, fetal mesangial cells change their immunohistochemical phenotypes with maturation. Consequently, we hypothesized that histological and immunohistochemical evaluations of the kidney can be used together as an indirect parameter for age estimation in puppies’ cadavers. Kidney samples from 45 cadavers were collected and processed for histopathological (for morphometrical study of the glomerulus) and immunohistochemical (for the immunolocalization of the α-smooth muscle actin protein—α-SMA) examinations. Morphometrical and immunohistochemical studies allowed us to observe differences among assessed groups. Our findings suggest a potential use of both kidney morphometrical and immunohistochemical examinations together as an indirect parameter to assess the age of puppies. ABSTRACT: Estimation of age represents a central focus in the veterinary forensic pathology field. Currently, the visual examination of the dentition and the skeletal age are the main methods to estimate the age of puppies. Nevertheless, these methods are affected by a broad range of variables. In contrast, the kidney is characterized by a specific postnatal development. In human glomerulogenesis, fetal mesangial cells change their immunohistochemical phenotypes with maturation. Therefore, we hypothesized that histological and immunohistochemical examinations of the kidney can be used together as an indirect parameter for age determination in puppies’ cadavers. Forty-five puppies’ cadavers were divided into five groups defined by age (Group A= 0–15 days, Group B = 16–45 days, Group C = 46–85 days, Group D = 86–105 days, Group E= 105–365 days). For each case, kidney samples were collected and processed for histopathological (for morphometrical study of the glomerulus) and immunohistochemical (for the immunolocalization of the α-SMA protein) studies. Morphometrical study allowed us to observe statistical differences in the mean glomerulus numbers per field among assessed groups. Similarly, immunohistochemical examination showed differences in SMA expression among groups. Our findings suggest a potential use of kidney morphometrical and immunohistochemical examinations together as an indirect parameter to assess the age of illegally imported puppies. |
format | Online Article Text |
id | pubmed-10451229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104512292023-08-26 Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology d’Aquino, Ilaria Piegari, Giuseppe Miletti, Gianluca Sannino, Emanuela Costanza, Dario Meomartino, Leonardo Fico, Rosario Riccio, Lorenzo Vaccaro, Emanuela De Biase, Davide Paciello, Orlando Animals (Basel) Article SIMPLE SUMMARY: Estimation of age represents a challenge in the veterinary forensic pathology field as well as in human forensic medicine. In puppies, the most common methods of age estimation are the visual examination of the dentition and the skeletal age. Nevertheless, these methods are affected by a broad range of variables. In contrast, the kidney is characterized by a precise postnatal development. In human glomerulogenesis, fetal mesangial cells change their immunohistochemical phenotypes with maturation. Consequently, we hypothesized that histological and immunohistochemical evaluations of the kidney can be used together as an indirect parameter for age estimation in puppies’ cadavers. Kidney samples from 45 cadavers were collected and processed for histopathological (for morphometrical study of the glomerulus) and immunohistochemical (for the immunolocalization of the α-smooth muscle actin protein—α-SMA) examinations. Morphometrical and immunohistochemical studies allowed us to observe differences among assessed groups. Our findings suggest a potential use of both kidney morphometrical and immunohistochemical examinations together as an indirect parameter to assess the age of puppies. ABSTRACT: Estimation of age represents a central focus in the veterinary forensic pathology field. Currently, the visual examination of the dentition and the skeletal age are the main methods to estimate the age of puppies. Nevertheless, these methods are affected by a broad range of variables. In contrast, the kidney is characterized by a specific postnatal development. In human glomerulogenesis, fetal mesangial cells change their immunohistochemical phenotypes with maturation. Therefore, we hypothesized that histological and immunohistochemical examinations of the kidney can be used together as an indirect parameter for age determination in puppies’ cadavers. Forty-five puppies’ cadavers were divided into five groups defined by age (Group A= 0–15 days, Group B = 16–45 days, Group C = 46–85 days, Group D = 86–105 days, Group E= 105–365 days). For each case, kidney samples were collected and processed for histopathological (for morphometrical study of the glomerulus) and immunohistochemical (for the immunolocalization of the α-SMA protein) studies. Morphometrical study allowed us to observe statistical differences in the mean glomerulus numbers per field among assessed groups. Similarly, immunohistochemical examination showed differences in SMA expression among groups. Our findings suggest a potential use of kidney morphometrical and immunohistochemical examinations together as an indirect parameter to assess the age of illegally imported puppies. MDPI 2023-08-18 /pmc/articles/PMC10451229/ /pubmed/37627455 http://dx.doi.org/10.3390/ani13162665 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article d’Aquino, Ilaria Piegari, Giuseppe Miletti, Gianluca Sannino, Emanuela Costanza, Dario Meomartino, Leonardo Fico, Rosario Riccio, Lorenzo Vaccaro, Emanuela De Biase, Davide Paciello, Orlando Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title | Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title_full | Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title_fullStr | Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title_full_unstemmed | Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title_short | Morphometrical and Immunohistochemical Evaluation of Kidney as an Indirect Parameter to Estimate Age in Puppies in Veterinary Forensic Pathology |
title_sort | morphometrical and immunohistochemical evaluation of kidney as an indirect parameter to estimate age in puppies in veterinary forensic pathology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451229/ https://www.ncbi.nlm.nih.gov/pubmed/37627455 http://dx.doi.org/10.3390/ani13162665 |
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