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Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats
Dogs have been reported as potential carriers of antimicrobial-resistant bacteria, but the role of cats has been poorly studied. The aim of this study was to investigate the presence and the risk factors associated with the fecal carriage of extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-produci...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451524/ https://www.ncbi.nlm.nih.gov/pubmed/37627669 http://dx.doi.org/10.3390/antibiotics12081249 |
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author | Ratti, Gabriele Facchin, Alessia Stranieri, Angelica Giordano, Alessia Paltrinieri, Saverio Scarpa, Paola Maragno, Deborah Gazzonis, Alessia Penati, Martina Luzzago, Camilla Dall’Ara, Paola Lauzi, Stefania |
author_facet | Ratti, Gabriele Facchin, Alessia Stranieri, Angelica Giordano, Alessia Paltrinieri, Saverio Scarpa, Paola Maragno, Deborah Gazzonis, Alessia Penati, Martina Luzzago, Camilla Dall’Ara, Paola Lauzi, Stefania |
author_sort | Ratti, Gabriele |
collection | PubMed |
description | Dogs have been reported as potential carriers of antimicrobial-resistant bacteria, but the role of cats has been poorly studied. The aim of this study was to investigate the presence and the risk factors associated with the fecal carriage of extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing Escherichia coli (E. coli) in pet and stray cats. Fecal samples were collected between 2020 and 2022 from healthy and unhealthy cats and screened for ESBL/AmpC-producing E. coli using selective media. The presence of ESBL/AmpC-producing E. coli was confirmed by phenotypic and molecular methods. The evaluation of minimum inhibitory concentrations (MICs) was performed on positive isolates. Host and hospitalization data were analyzed to identify risk factors. A total of 97 cats’ samples were collected, and ESBL/AmpC-producing E. coli were detected in 6/97 (6.2%), supported by the detection of bla(CTX-M) (100%), bla(TEM) (83.3%), and bla(SHV) (16.7%) genes and the overexpression of chromosomal ampC (1%). All E. coli isolates were categorized as multidrug-resistant. Unhealthy status and previous antibiotic therapy were significantly associated with ESBL/AmpC-producing E. coli fecal carriage. Our results suggest that cats may be carriers of ESBL/AmpC-producing E. coli, highlighting the need for antimicrobial stewardship in veterinary medicine and an antimicrobial-resistance surveillance program focusing on companion animals, including stray cats. |
format | Online Article Text |
id | pubmed-10451524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104515242023-08-26 Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats Ratti, Gabriele Facchin, Alessia Stranieri, Angelica Giordano, Alessia Paltrinieri, Saverio Scarpa, Paola Maragno, Deborah Gazzonis, Alessia Penati, Martina Luzzago, Camilla Dall’Ara, Paola Lauzi, Stefania Antibiotics (Basel) Article Dogs have been reported as potential carriers of antimicrobial-resistant bacteria, but the role of cats has been poorly studied. The aim of this study was to investigate the presence and the risk factors associated with the fecal carriage of extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing Escherichia coli (E. coli) in pet and stray cats. Fecal samples were collected between 2020 and 2022 from healthy and unhealthy cats and screened for ESBL/AmpC-producing E. coli using selective media. The presence of ESBL/AmpC-producing E. coli was confirmed by phenotypic and molecular methods. The evaluation of minimum inhibitory concentrations (MICs) was performed on positive isolates. Host and hospitalization data were analyzed to identify risk factors. A total of 97 cats’ samples were collected, and ESBL/AmpC-producing E. coli were detected in 6/97 (6.2%), supported by the detection of bla(CTX-M) (100%), bla(TEM) (83.3%), and bla(SHV) (16.7%) genes and the overexpression of chromosomal ampC (1%). All E. coli isolates were categorized as multidrug-resistant. Unhealthy status and previous antibiotic therapy were significantly associated with ESBL/AmpC-producing E. coli fecal carriage. Our results suggest that cats may be carriers of ESBL/AmpC-producing E. coli, highlighting the need for antimicrobial stewardship in veterinary medicine and an antimicrobial-resistance surveillance program focusing on companion animals, including stray cats. MDPI 2023-07-29 /pmc/articles/PMC10451524/ /pubmed/37627669 http://dx.doi.org/10.3390/antibiotics12081249 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ratti, Gabriele Facchin, Alessia Stranieri, Angelica Giordano, Alessia Paltrinieri, Saverio Scarpa, Paola Maragno, Deborah Gazzonis, Alessia Penati, Martina Luzzago, Camilla Dall’Ara, Paola Lauzi, Stefania Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title | Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title_full | Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title_fullStr | Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title_full_unstemmed | Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title_short | Fecal Carriage of Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli in Pet and Stray Cats |
title_sort | fecal carriage of extended-spectrum β-lactamase-/ampc-producing escherichia coli in pet and stray cats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451524/ https://www.ncbi.nlm.nih.gov/pubmed/37627669 http://dx.doi.org/10.3390/antibiotics12081249 |
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