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Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature

Evidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old db/db diabetic male mice were fed a diet s...

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Autores principales: Satheesh Babu, Adhini Kuppuswamy, Petersen, Chrissa, Paz, Henry A., Benedict, Kai, Nguyen, Miley, Putich, Madison, Saldivar-Gonzalez, Miguel, Zhong, Ying, Larsen, Sydney, Wankhade, Umesh D., Anandh Babu, Pon Velayutham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451530/
https://www.ncbi.nlm.nih.gov/pubmed/37627522
http://dx.doi.org/10.3390/antiox12081527
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author Satheesh Babu, Adhini Kuppuswamy
Petersen, Chrissa
Paz, Henry A.
Benedict, Kai
Nguyen, Miley
Putich, Madison
Saldivar-Gonzalez, Miguel
Zhong, Ying
Larsen, Sydney
Wankhade, Umesh D.
Anandh Babu, Pon Velayutham
author_facet Satheesh Babu, Adhini Kuppuswamy
Petersen, Chrissa
Paz, Henry A.
Benedict, Kai
Nguyen, Miley
Putich, Madison
Saldivar-Gonzalez, Miguel
Zhong, Ying
Larsen, Sydney
Wankhade, Umesh D.
Anandh Babu, Pon Velayutham
author_sort Satheesh Babu, Adhini Kuppuswamy
collection PubMed
description Evidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old db/db diabetic male mice were fed a diet supplemented with ± freeze-dried wild blueberry powder (FD-BB) for 4, 8, or 12 weeks (three cohorts). Diets contained 0%, 1.23%, 2.46%, and 3.7% of FD-BB, equivalent to 0, ½, 1, and 1.5 human servings of wild blueberries, respectively. The non-diabetic db/+ mice fed a standard diet served as controls. Metabolic parameters, vascular inflammation, and gut microbiome were assessed. Dietary supplementation of 3.7% FD-BB improved vascular inflammation in diabetic mice without improving systemic milieu in all three cohorts. Blueberries improved diabetes-induced gut dysbiosis depending on blueberry dosage and treatment duration. Spearman’s correlation indicated that the opportunistic microbes and commensal microbes were positively and negatively associated with indices of vascular inflammation, respectively. Dietary blueberries reduced the opportunistic microbe that was positively associated with vascular inflammation (Desulfovibrio), and increased the commensal microbe that was negatively associated with vascular inflammation (Akkermansia). Dietary blueberries could be a potential adjunct strategy to beneficially modulate gut microbes and improve vascular complications in diabetes.
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spelling pubmed-104515302023-08-26 Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature Satheesh Babu, Adhini Kuppuswamy Petersen, Chrissa Paz, Henry A. Benedict, Kai Nguyen, Miley Putich, Madison Saldivar-Gonzalez, Miguel Zhong, Ying Larsen, Sydney Wankhade, Umesh D. Anandh Babu, Pon Velayutham Antioxidants (Basel) Article Evidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old db/db diabetic male mice were fed a diet supplemented with ± freeze-dried wild blueberry powder (FD-BB) for 4, 8, or 12 weeks (three cohorts). Diets contained 0%, 1.23%, 2.46%, and 3.7% of FD-BB, equivalent to 0, ½, 1, and 1.5 human servings of wild blueberries, respectively. The non-diabetic db/+ mice fed a standard diet served as controls. Metabolic parameters, vascular inflammation, and gut microbiome were assessed. Dietary supplementation of 3.7% FD-BB improved vascular inflammation in diabetic mice without improving systemic milieu in all three cohorts. Blueberries improved diabetes-induced gut dysbiosis depending on blueberry dosage and treatment duration. Spearman’s correlation indicated that the opportunistic microbes and commensal microbes were positively and negatively associated with indices of vascular inflammation, respectively. Dietary blueberries reduced the opportunistic microbe that was positively associated with vascular inflammation (Desulfovibrio), and increased the commensal microbe that was negatively associated with vascular inflammation (Akkermansia). Dietary blueberries could be a potential adjunct strategy to beneficially modulate gut microbes and improve vascular complications in diabetes. MDPI 2023-07-30 /pmc/articles/PMC10451530/ /pubmed/37627522 http://dx.doi.org/10.3390/antiox12081527 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Satheesh Babu, Adhini Kuppuswamy
Petersen, Chrissa
Paz, Henry A.
Benedict, Kai
Nguyen, Miley
Putich, Madison
Saldivar-Gonzalez, Miguel
Zhong, Ying
Larsen, Sydney
Wankhade, Umesh D.
Anandh Babu, Pon Velayutham
Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title_full Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title_fullStr Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title_full_unstemmed Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title_short Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
title_sort dose- and time-dependent effect of dietary blueberries on diabetic vasculature is correlated with gut microbial signature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451530/
https://www.ncbi.nlm.nih.gov/pubmed/37627522
http://dx.doi.org/10.3390/antiox12081527
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