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Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals
This work presents a new method using kinetic potentiometry to study the thermodynamic and kinetic parameters of the reactions of antioxidants with peroxyl radicals. The rate constants of the reaction of antioxidants with radicals have been determined, and the groups of “fast” and “slow” antioxidant...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451547/ https://www.ncbi.nlm.nih.gov/pubmed/37627605 http://dx.doi.org/10.3390/antiox12081608 |
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author | Gerasimova, Elena Salimgareeva, Elena Magasumova, Dinara Ivanova, Alla |
author_facet | Gerasimova, Elena Salimgareeva, Elena Magasumova, Dinara Ivanova, Alla |
author_sort | Gerasimova, Elena |
collection | PubMed |
description | This work presents a new method using kinetic potentiometry to study the thermodynamic and kinetic parameters of the reactions of antioxidants with peroxyl radicals. The rate constants of the reaction of antioxidants with radicals have been determined, and the groups of “fast” and “slow” antioxidants have been conventionally distinguished. Fast antioxidants include ascorbic, uric, gallic, chlorogenic, caffeic acids, glutathione, L-cysteine, and catechol with constant values from (1.05–9.25) × 10(3) M·s(−1); “slow” antioxidants are α-tocopherol (in aqueous media), ionol, 2,6-ditretbutylphenol, and compounds of the azoloazine series, modified with polyphenolic fragments, with constant values from (4.00–8.50) × 10(2) M·s(−1). It is shown that the value of the rate constant is directly related to the type of kinetic dependence of the potential recorded when an antioxidant is introduced into the solution of the radical initiator. It is shown that the method with the determination of the induction period is difficult in the study of “slow” antioxidants. It has been established that the area above the curve of the kinetic dependence Exp(∆E) is directly related to the amount of inhibited peroxyl radicals and can be used to assess the inhibitory properties of an antioxidant from a thermodynamic point of view. “Fixed time method” and “Initial rate method” were used. Positive correlations between the described method have been established. The utility of the parameter of the area above the curve of the kinetic dependence Exp(∆E) in the study of objects of complex composition is shown. |
format | Online Article Text |
id | pubmed-10451547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104515472023-08-26 Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals Gerasimova, Elena Salimgareeva, Elena Magasumova, Dinara Ivanova, Alla Antioxidants (Basel) Article This work presents a new method using kinetic potentiometry to study the thermodynamic and kinetic parameters of the reactions of antioxidants with peroxyl radicals. The rate constants of the reaction of antioxidants with radicals have been determined, and the groups of “fast” and “slow” antioxidants have been conventionally distinguished. Fast antioxidants include ascorbic, uric, gallic, chlorogenic, caffeic acids, glutathione, L-cysteine, and catechol with constant values from (1.05–9.25) × 10(3) M·s(−1); “slow” antioxidants are α-tocopherol (in aqueous media), ionol, 2,6-ditretbutylphenol, and compounds of the azoloazine series, modified with polyphenolic fragments, with constant values from (4.00–8.50) × 10(2) M·s(−1). It is shown that the value of the rate constant is directly related to the type of kinetic dependence of the potential recorded when an antioxidant is introduced into the solution of the radical initiator. It is shown that the method with the determination of the induction period is difficult in the study of “slow” antioxidants. It has been established that the area above the curve of the kinetic dependence Exp(∆E) is directly related to the amount of inhibited peroxyl radicals and can be used to assess the inhibitory properties of an antioxidant from a thermodynamic point of view. “Fixed time method” and “Initial rate method” were used. Positive correlations between the described method have been established. The utility of the parameter of the area above the curve of the kinetic dependence Exp(∆E) in the study of objects of complex composition is shown. MDPI 2023-08-14 /pmc/articles/PMC10451547/ /pubmed/37627605 http://dx.doi.org/10.3390/antiox12081608 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gerasimova, Elena Salimgareeva, Elena Magasumova, Dinara Ivanova, Alla Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title | Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title_full | Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title_fullStr | Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title_full_unstemmed | Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title_short | Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals |
title_sort | kinetic potentiometry as a method for studying the interactions of antioxidants with peroxyl radicals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451547/ https://www.ncbi.nlm.nih.gov/pubmed/37627605 http://dx.doi.org/10.3390/antiox12081608 |
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