High-Throughput Assay of Cytochrome P450-Dependent Drug Demethylation Reactions and Its Use to Re-Evaluate the Pathways of Ketamine Metabolism

SIMPLE SUMMARY: Here, we introduce a reliable, inexpensive, and versatile method for high-throughput kinetic assays of drug metabolism based on fluorometric quantification of formaldehyde (FA) formed in cytochrome P450-dependent demethylation reactions. We describe the implementation of this techniq...

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Detalles Bibliográficos
Autores principales: Davydova, Nadezhda Y., Hutner, David A., Gaither, Kari A., Singh, Dilip Kumar, Prasad, Bhagwat, Davydov, Dmitri R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451610/
https://www.ncbi.nlm.nih.gov/pubmed/37626940
http://dx.doi.org/10.3390/biology12081055

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451610/
https://www.ncbi.nlm.nih.gov/pubmed/37626940
http://dx.doi.org/10.3390/biology12081055

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