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Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress

SIMPLE SUMMARY: Oxidatively stressed sperm can fertilise oocytes and, hence, transfer its damaged genome to a progeny. Sperm cannot repair its own genome, therefore the oocyte’s capacity to deal with damaged sperm influences the outcome of a fertilisation. We distinguished bovine oocytes of differen...

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Autores principales: Bittner-Schwerda, Lilli, Herrera, Carolina, Wyck, Sarah, Malama, Eleni, Wrenzycki, Christine, Bollwein, Heinrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451622/
https://www.ncbi.nlm.nih.gov/pubmed/37627412
http://dx.doi.org/10.3390/ani13162621
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author Bittner-Schwerda, Lilli
Herrera, Carolina
Wyck, Sarah
Malama, Eleni
Wrenzycki, Christine
Bollwein, Heinrich
author_facet Bittner-Schwerda, Lilli
Herrera, Carolina
Wyck, Sarah
Malama, Eleni
Wrenzycki, Christine
Bollwein, Heinrich
author_sort Bittner-Schwerda, Lilli
collection PubMed
description SIMPLE SUMMARY: Oxidatively stressed sperm can fertilise oocytes and, hence, transfer its damaged genome to a progeny. Sperm cannot repair its own genome, therefore the oocyte’s capacity to deal with damaged sperm influences the outcome of a fertilisation. We distinguished bovine oocytes of different qualities in vitro using Brilliant Cresyl Blue (BCB) staining and in vitro fertilised them with non-oxidised and oxidised bovine semen. BCB-negative oocytes were less able to support embryo development after fertilisation with oxidatively stressed bovine semen, demonstrated by lower cleavage and blastocyst rates, a delay in development and lower blastocyst quality. These results support a further description of the oocytes’ quality in regard of their capacity to deal with damaged sperm, which will help in selecting the best oocytes when damaged sperm have to be used in assisted reproduction. ABSTRACT: The extent of oxidative damage transferred by the damaged sperm to the progeny is likely to be limited by the oocyte’s repair and antioxidative capacity. We aimed to assess the association between Brilliant Cresyl Blue (BCB) staining in oocytes and their competence for embryo development after in vitro fertilisation (IVF) with damaged sperm. For this purpose, bovine sperm were incubated without (non-oxidised sperm, NOX S) or with 100 µM H(2)O(2) (oxidised sperm, OX S) and were used to fertilise in-vitro-matured bovine oocytes (BCB-pos./BCB-neg.). Unstained oocytes served as controls (US). Development was assessed at 30, 46, 60 h and on Days (D) 7 and 8 after IVF. Total cell number and apoptotic index were analysed in D7 blastocysts. BCB-neg. oocytes showed lower cleavage rates and blastocyst rates than unstained oocytes after IVF with NOX S (p < 0.05). They showed the highest reduction in D7 blastocyst rate upon fertilisation with OX S and showed a delayed embryo development at 46 and 60 h after IVF compared to embryos produced with NOX S (p < 0.05). Total cell number in blastocysts produced with BCB-neg. oocytes was lower (p < 0.05) in the embryos produced with OX S than in embryos after IVF with NOX S. In conclusion, BCB-neg. oocytes have a lower competence to support embryo development after in vitro fertilisation with oxidised sperm.
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spelling pubmed-104516222023-08-26 Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress Bittner-Schwerda, Lilli Herrera, Carolina Wyck, Sarah Malama, Eleni Wrenzycki, Christine Bollwein, Heinrich Animals (Basel) Article SIMPLE SUMMARY: Oxidatively stressed sperm can fertilise oocytes and, hence, transfer its damaged genome to a progeny. Sperm cannot repair its own genome, therefore the oocyte’s capacity to deal with damaged sperm influences the outcome of a fertilisation. We distinguished bovine oocytes of different qualities in vitro using Brilliant Cresyl Blue (BCB) staining and in vitro fertilised them with non-oxidised and oxidised bovine semen. BCB-negative oocytes were less able to support embryo development after fertilisation with oxidatively stressed bovine semen, demonstrated by lower cleavage and blastocyst rates, a delay in development and lower blastocyst quality. These results support a further description of the oocytes’ quality in regard of their capacity to deal with damaged sperm, which will help in selecting the best oocytes when damaged sperm have to be used in assisted reproduction. ABSTRACT: The extent of oxidative damage transferred by the damaged sperm to the progeny is likely to be limited by the oocyte’s repair and antioxidative capacity. We aimed to assess the association between Brilliant Cresyl Blue (BCB) staining in oocytes and their competence for embryo development after in vitro fertilisation (IVF) with damaged sperm. For this purpose, bovine sperm were incubated without (non-oxidised sperm, NOX S) or with 100 µM H(2)O(2) (oxidised sperm, OX S) and were used to fertilise in-vitro-matured bovine oocytes (BCB-pos./BCB-neg.). Unstained oocytes served as controls (US). Development was assessed at 30, 46, 60 h and on Days (D) 7 and 8 after IVF. Total cell number and apoptotic index were analysed in D7 blastocysts. BCB-neg. oocytes showed lower cleavage rates and blastocyst rates than unstained oocytes after IVF with NOX S (p < 0.05). They showed the highest reduction in D7 blastocyst rate upon fertilisation with OX S and showed a delayed embryo development at 46 and 60 h after IVF compared to embryos produced with NOX S (p < 0.05). Total cell number in blastocysts produced with BCB-neg. oocytes was lower (p < 0.05) in the embryos produced with OX S than in embryos after IVF with NOX S. In conclusion, BCB-neg. oocytes have a lower competence to support embryo development after in vitro fertilisation with oxidised sperm. MDPI 2023-08-14 /pmc/articles/PMC10451622/ /pubmed/37627412 http://dx.doi.org/10.3390/ani13162621 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bittner-Schwerda, Lilli
Herrera, Carolina
Wyck, Sarah
Malama, Eleni
Wrenzycki, Christine
Bollwein, Heinrich
Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title_full Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title_fullStr Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title_full_unstemmed Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title_short Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress
title_sort brilliant cresyl blue negative oocytes show a reduced competence for embryo development after in vitro fertilisation with sperm exposed to oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451622/
https://www.ncbi.nlm.nih.gov/pubmed/37627412
http://dx.doi.org/10.3390/ani13162621
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