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New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study

Quorum quenching (QQ) is the inhibition of bacterial communication, i.e., quorum sensing (QS). QS is a key mechanism in regulating biofilm formation and phenotype in complex bacterial communities, such as those found within cariogenic biofilms. Whereas QQ approaches were shown to effectively reduce...

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Autores principales: Parga, Ana, Balboa, Sabela, Otero-Casal, Paz, Otero, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451667/
https://www.ncbi.nlm.nih.gov/pubmed/37627682
http://dx.doi.org/10.3390/antibiotics12081263
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author Parga, Ana
Balboa, Sabela
Otero-Casal, Paz
Otero, Ana
author_facet Parga, Ana
Balboa, Sabela
Otero-Casal, Paz
Otero, Ana
author_sort Parga, Ana
collection PubMed
description Quorum quenching (QQ) is the inhibition of bacterial communication, i.e., quorum sensing (QS). QS is a key mechanism in regulating biofilm formation and phenotype in complex bacterial communities, such as those found within cariogenic biofilms. Whereas QQ approaches were shown to effectively reduce biomass, knowledge of their impact on the taxonomic composition of oral polymicrobial biofilms remains scarce. Here, we investigate the effect of the QQ lactonase Aii20J on biomass production and taxonomical composition of biofilms. We collected supragingival plaque samples from 10 caries-free and 10 caries-active children and cultured them to generate in vitro biofilms. We describe significant biomass reductions upon Aii20J exposure, as assessed by crystal violet assays. Taxonomical profiling using 16S rRNA gene amplicon sequencing revealed no significant changes in bacterial composition at the genus level. Interestingly, at the species level Aii20J-treatment increased the abundance of Streptococcus cristatus and Streptococcus salivarius. Both S. cristatus and S. salivarius express pH-buffering enzymes (arginine deiminase and urease, respectively) that catalyze ammonia production, thereby potentially raising local pH and counteracting the biofilm’s cariogenic potential. Within the limitations of the study, our findings provide evidence of the biofilm-modulating ability of QQ and offer novel insights into alternative strategies to restore homeostasis within dysbiotic ecosystems.
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spelling pubmed-104516672023-08-26 New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study Parga, Ana Balboa, Sabela Otero-Casal, Paz Otero, Ana Antibiotics (Basel) Article Quorum quenching (QQ) is the inhibition of bacterial communication, i.e., quorum sensing (QS). QS is a key mechanism in regulating biofilm formation and phenotype in complex bacterial communities, such as those found within cariogenic biofilms. Whereas QQ approaches were shown to effectively reduce biomass, knowledge of their impact on the taxonomic composition of oral polymicrobial biofilms remains scarce. Here, we investigate the effect of the QQ lactonase Aii20J on biomass production and taxonomical composition of biofilms. We collected supragingival plaque samples from 10 caries-free and 10 caries-active children and cultured them to generate in vitro biofilms. We describe significant biomass reductions upon Aii20J exposure, as assessed by crystal violet assays. Taxonomical profiling using 16S rRNA gene amplicon sequencing revealed no significant changes in bacterial composition at the genus level. Interestingly, at the species level Aii20J-treatment increased the abundance of Streptococcus cristatus and Streptococcus salivarius. Both S. cristatus and S. salivarius express pH-buffering enzymes (arginine deiminase and urease, respectively) that catalyze ammonia production, thereby potentially raising local pH and counteracting the biofilm’s cariogenic potential. Within the limitations of the study, our findings provide evidence of the biofilm-modulating ability of QQ and offer novel insights into alternative strategies to restore homeostasis within dysbiotic ecosystems. MDPI 2023-07-31 /pmc/articles/PMC10451667/ /pubmed/37627682 http://dx.doi.org/10.3390/antibiotics12081263 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parga, Ana
Balboa, Sabela
Otero-Casal, Paz
Otero, Ana
New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title_full New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title_fullStr New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title_full_unstemmed New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title_short New Preventive Strategy against Oral Biofilm Formation in Caries-Active Children: An In Vitro Study
title_sort new preventive strategy against oral biofilm formation in caries-active children: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451667/
https://www.ncbi.nlm.nih.gov/pubmed/37627682
http://dx.doi.org/10.3390/antibiotics12081263
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