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Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages

Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicit...

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Autores principales: Ventura, Célia, Pinto, Fátima, Lourenço, Ana Filipa, Pedrosa, Jorge F. S., Fernandes, Susete N., da Rosa, Rafaela R., Godinho, Maria Helena, Ferreira, Paulo J. T., Louro, Henriqueta, Silva, Maria João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452089/
https://www.ncbi.nlm.nih.gov/pubmed/37627871
http://dx.doi.org/10.3390/bioengineering10080986
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author Ventura, Célia
Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F. S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J. T.
Louro, Henriqueta
Silva, Maria João
author_facet Ventura, Célia
Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F. S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J. T.
Louro, Henriqueta
Silva, Maria João
author_sort Ventura, Célia
collection PubMed
description Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential.
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spelling pubmed-104520892023-08-26 Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages Ventura, Célia Pinto, Fátima Lourenço, Ana Filipa Pedrosa, Jorge F. S. Fernandes, Susete N. da Rosa, Rafaela R. Godinho, Maria Helena Ferreira, Paulo J. T. Louro, Henriqueta Silva, Maria João Bioengineering (Basel) Article Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential. MDPI 2023-08-21 /pmc/articles/PMC10452089/ /pubmed/37627871 http://dx.doi.org/10.3390/bioengineering10080986 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ventura, Célia
Pinto, Fátima
Lourenço, Ana Filipa
Pedrosa, Jorge F. S.
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J. T.
Louro, Henriqueta
Silva, Maria João
Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_full Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_fullStr Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_full_unstemmed Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_short Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
title_sort assessing the genotoxicity of cellulose nanomaterials in a co-culture of human lung epithelial cells and monocyte-derived macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452089/
https://www.ncbi.nlm.nih.gov/pubmed/37627871
http://dx.doi.org/10.3390/bioengineering10080986
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