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The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques

Various functions of polymorphonuclear neutrophils (PMNs) are related to diseases and postoperative plasma changes. The influence of some local anesthetics (LAs) on PMNs obtained by conventional isolation methods and their functions has already been demonstrated. This study investigates the effect o...

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Autores principales: Sixt, Sara, Gruber, Michael, Kolle, Gesche, Galla, Thies, Bitzinger, Diane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452207/
https://www.ncbi.nlm.nih.gov/pubmed/37626667
http://dx.doi.org/10.3390/biomedicines11082170
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author Sixt, Sara
Gruber, Michael
Kolle, Gesche
Galla, Thies
Bitzinger, Diane
author_facet Sixt, Sara
Gruber, Michael
Kolle, Gesche
Galla, Thies
Bitzinger, Diane
author_sort Sixt, Sara
collection PubMed
description Various functions of polymorphonuclear neutrophils (PMNs) are related to diseases and postoperative plasma changes. The influence of some local anesthetics (LAs) on PMNs obtained by conventional isolation methods and their functions has already been demonstrated. This study investigates the effect of selected LAs on PMNs, comparing a new isolation method with conventional ones. To obtain the PMNs, we performed either gelafundin sedimentation, hypotonic lysis or density gradient centrifugation. Subsequently, PMNs were mixed with different concentrations of bupivacaine, levobupivacaine, lidocaine or ropivacaine. Live cell imaging and flow cytometry were performed to quantify the migration, ROS production, NETosis and antigen expression of PMNs. We found the inhibition of chemotaxis and ROS production by LAs. PMNs showed a strong reduction in time to half maximal NETosis in response to bupivacaine and lidocaine, but not to levobupivacaine and ropivacaine. We also found distinct differences in survival time and migration duration between the isolation methods. This suggests that the careful selection of LAs has a short-term impact on in vitro PMNs.
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spelling pubmed-104522072023-08-26 The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques Sixt, Sara Gruber, Michael Kolle, Gesche Galla, Thies Bitzinger, Diane Biomedicines Article Various functions of polymorphonuclear neutrophils (PMNs) are related to diseases and postoperative plasma changes. The influence of some local anesthetics (LAs) on PMNs obtained by conventional isolation methods and their functions has already been demonstrated. This study investigates the effect of selected LAs on PMNs, comparing a new isolation method with conventional ones. To obtain the PMNs, we performed either gelafundin sedimentation, hypotonic lysis or density gradient centrifugation. Subsequently, PMNs were mixed with different concentrations of bupivacaine, levobupivacaine, lidocaine or ropivacaine. Live cell imaging and flow cytometry were performed to quantify the migration, ROS production, NETosis and antigen expression of PMNs. We found the inhibition of chemotaxis and ROS production by LAs. PMNs showed a strong reduction in time to half maximal NETosis in response to bupivacaine and lidocaine, but not to levobupivacaine and ropivacaine. We also found distinct differences in survival time and migration duration between the isolation methods. This suggests that the careful selection of LAs has a short-term impact on in vitro PMNs. MDPI 2023-08-02 /pmc/articles/PMC10452207/ /pubmed/37626667 http://dx.doi.org/10.3390/biomedicines11082170 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sixt, Sara
Gruber, Michael
Kolle, Gesche
Galla, Thies
Bitzinger, Diane
The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title_full The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title_fullStr The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title_full_unstemmed The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title_short The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques
title_sort effect of local anesthetics on neutrophils in the context of different isolation techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452207/
https://www.ncbi.nlm.nih.gov/pubmed/37626667
http://dx.doi.org/10.3390/biomedicines11082170
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