Cargando…

Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy

Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen o...

Descripción completa

Detalles Bibliográficos
Autores principales: Gigli, Lucia, Braidotti, Nicoletta, Lima, Maria Augusta do R. B. F., Ciubotaru, Catalin Dacian, Cojoc, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452265/
https://www.ncbi.nlm.nih.gov/pubmed/37622874
http://dx.doi.org/10.3390/bios13080789
_version_ 1785095626194681856
author Gigli, Lucia
Braidotti, Nicoletta
Lima, Maria Augusta do R. B. F.
Ciubotaru, Catalin Dacian
Cojoc, Dan
author_facet Gigli, Lucia
Braidotti, Nicoletta
Lima, Maria Augusta do R. B. F.
Ciubotaru, Catalin Dacian
Cojoc, Dan
author_sort Gigli, Lucia
collection PubMed
description Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen out the negative samples. We propose a digital holographic microscopy (DHM) method to inspect fresh urine samples flowing in a glass capillary for 3 min, recording holograms at 2 frames per second. After digital reconstruction, bacteria, white and red blood cells, epithelial cells and crystals were identified and counted, and the samples were classified as negative or positive according to clinical cutoff values. Taking the CPT as reference, we processed 180 urine samples and compared the results with those of urine flow cytometry (UFC). Using standard evaluation metrics for our screening test, we found a similar performance for DHM and UFC, indicating DHM as a suitable and fast screening technique retaining several advantages. As a benefit of DHM, the technique is label-free and does not require sample preparation. Moreover, the phase and amplitude images of the cells and other particles present in urine are digitally recorded and can serve for further investigation afterwards.
format Online
Article
Text
id pubmed-10452265
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104522652023-08-26 Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy Gigli, Lucia Braidotti, Nicoletta Lima, Maria Augusta do R. B. F. Ciubotaru, Catalin Dacian Cojoc, Dan Biosensors (Basel) Article Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen out the negative samples. We propose a digital holographic microscopy (DHM) method to inspect fresh urine samples flowing in a glass capillary for 3 min, recording holograms at 2 frames per second. After digital reconstruction, bacteria, white and red blood cells, epithelial cells and crystals were identified and counted, and the samples were classified as negative or positive according to clinical cutoff values. Taking the CPT as reference, we processed 180 urine samples and compared the results with those of urine flow cytometry (UFC). Using standard evaluation metrics for our screening test, we found a similar performance for DHM and UFC, indicating DHM as a suitable and fast screening technique retaining several advantages. As a benefit of DHM, the technique is label-free and does not require sample preparation. Moreover, the phase and amplitude images of the cells and other particles present in urine are digitally recorded and can serve for further investigation afterwards. MDPI 2023-08-04 /pmc/articles/PMC10452265/ /pubmed/37622874 http://dx.doi.org/10.3390/bios13080789 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gigli, Lucia
Braidotti, Nicoletta
Lima, Maria Augusta do R. B. F.
Ciubotaru, Catalin Dacian
Cojoc, Dan
Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title_full Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title_fullStr Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title_full_unstemmed Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title_short Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy
title_sort label-free analysis of urine samples with in-flow digital holographic microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452265/
https://www.ncbi.nlm.nih.gov/pubmed/37622874
http://dx.doi.org/10.3390/bios13080789
work_keys_str_mv AT giglilucia labelfreeanalysisofurinesampleswithinflowdigitalholographicmicroscopy
AT braidottinicoletta labelfreeanalysisofurinesampleswithinflowdigitalholographicmicroscopy
AT limamariaaugustadorbf labelfreeanalysisofurinesampleswithinflowdigitalholographicmicroscopy
AT ciubotarucatalindacian labelfreeanalysisofurinesampleswithinflowdigitalholographicmicroscopy
AT cojocdan labelfreeanalysisofurinesampleswithinflowdigitalholographicmicroscopy