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A Critical Study on DNA Probes Attached to Microplate for CRISPR/Cas12 Trans-Cleavage Activity

CRISPR/Cas12-based biosensors are emerging tools for diagnostics. However, their application of heterogeneous formats needs the efficient detection of Cas12 activity. We investigated DNA probes attached to the microplate surface and cleaved by Cas12a. Single-stranded (ss) DNA probes (19 variants) an...

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Detalles Bibliográficos
Autores principales:	Burkin, Konstantin M., Ivanov, Aleksandr V., Zherdev, Anatoly V., Dzantiev, Boris B., Safenkova, Irina V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452489/ https://www.ncbi.nlm.nih.gov/pubmed/37622910 http://dx.doi.org/10.3390/bios13080824

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