Cargando…

Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor

In this work, we present an accessible benchtop fabrication technique to obtain a planar array of gold nanowrinkled surface electrodes (ANSE) for the construction of electrochemical cells, specifically to monitor soluble biomarkers of interest in cell culture environments. We present a complete char...

Descripción completa

Detalles Bibliográficos
Autores principales: Peto-Gutiérrez, Cindy, Vázquez-Victorio, Genaro, Hautefeuille, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452632/
https://www.ncbi.nlm.nih.gov/pubmed/37622879
http://dx.doi.org/10.3390/bios13080794
_version_ 1785095718079299584
author Peto-Gutiérrez, Cindy
Vázquez-Victorio, Genaro
Hautefeuille, Mathieu
author_facet Peto-Gutiérrez, Cindy
Vázquez-Victorio, Genaro
Hautefeuille, Mathieu
author_sort Peto-Gutiérrez, Cindy
collection PubMed
description In this work, we present an accessible benchtop fabrication technique to obtain a planar array of gold nanowrinkled surface electrodes (ANSE) for the construction of electrochemical cells, specifically to monitor soluble biomarkers of interest in cell culture environments. We present a complete characterization of the array and its response as an electrochemical cell. To validate our sensor, we evaluated the device sensitivity to detect nitric oxide (NO), an important molecule produced by endothelial cells as a response to environmental signals such as mechanics and growth factors. While testing measurements of nitric oxide in aqueous solutions with isotonic salt concentrations, we evidenced the influence of the environmental conditions for such electrochemical measurements, showing that the aqueous medium, usually not accounted for, significantly impacts the outcome. Finally, we present the application of the electrochemical sensor for the detection of nitric oxide released from stimulated endothelial cells as a proof of concept.
format Online
Article
Text
id pubmed-10452632
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104526322023-08-26 Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor Peto-Gutiérrez, Cindy Vázquez-Victorio, Genaro Hautefeuille, Mathieu Biosensors (Basel) Article In this work, we present an accessible benchtop fabrication technique to obtain a planar array of gold nanowrinkled surface electrodes (ANSE) for the construction of electrochemical cells, specifically to monitor soluble biomarkers of interest in cell culture environments. We present a complete characterization of the array and its response as an electrochemical cell. To validate our sensor, we evaluated the device sensitivity to detect nitric oxide (NO), an important molecule produced by endothelial cells as a response to environmental signals such as mechanics and growth factors. While testing measurements of nitric oxide in aqueous solutions with isotonic salt concentrations, we evidenced the influence of the environmental conditions for such electrochemical measurements, showing that the aqueous medium, usually not accounted for, significantly impacts the outcome. Finally, we present the application of the electrochemical sensor for the detection of nitric oxide released from stimulated endothelial cells as a proof of concept. MDPI 2023-08-07 /pmc/articles/PMC10452632/ /pubmed/37622879 http://dx.doi.org/10.3390/bios13080794 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peto-Gutiérrez, Cindy
Vázquez-Victorio, Genaro
Hautefeuille, Mathieu
Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title_full Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title_fullStr Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title_full_unstemmed Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title_short Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor
title_sort characterization of benchtop-fabricated arrays of nanowrinkled surface electrodes as a nitric oxide electrochemical sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452632/
https://www.ncbi.nlm.nih.gov/pubmed/37622879
http://dx.doi.org/10.3390/bios13080794
work_keys_str_mv AT petogutierrezcindy characterizationofbenchtopfabricatedarraysofnanowrinkledsurfaceelectrodesasanitricoxideelectrochemicalsensor
AT vazquezvictoriogenaro characterizationofbenchtopfabricatedarraysofnanowrinkledsurfaceelectrodesasanitricoxideelectrochemicalsensor
AT hautefeuillemathieu characterizationofbenchtopfabricatedarraysofnanowrinkledsurfaceelectrodesasanitricoxideelectrochemicalsensor