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Circulating and Exosomal microRNA-33 in Childhood Obesity
Background: MicroRNA-33 may control a wide range of different metabolic functions. Methods: This study aims to assess the miR-33a circulating profile in normal-weight (N = 20) and obese (O = 30) adolescents and to correlate its expression levels to their metabolic parameters. In a subset of subjects...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452681/ https://www.ncbi.nlm.nih.gov/pubmed/37626791 http://dx.doi.org/10.3390/biomedicines11082295 |
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author | Cabiati, Manuela Guiducci, Letizia Randazzo, Emioli Casieri, Valentina Federico, Giovanni Del Ry, Silvia |
author_facet | Cabiati, Manuela Guiducci, Letizia Randazzo, Emioli Casieri, Valentina Federico, Giovanni Del Ry, Silvia |
author_sort | Cabiati, Manuela |
collection | PubMed |
description | Background: MicroRNA-33 may control a wide range of different metabolic functions. Methods: This study aims to assess the miR-33a circulating profile in normal-weight (N = 20) and obese (O = 30) adolescents and to correlate its expression levels to their metabolic parameters. In a subset of subjects, we compared circulating miR-33a with exosomal miR-33a. Results: Metabolic parameters were altered in O, with initial hyperinsulinemia. Circulating miR-33a was significantly higher in O than in N (p = 0.0002). Significant correlations between miR-33a and auxological and metabolic indices (Insulin p = 0.01; Cholesterol p = 0.01; LDL p = 0.01; HbA1c p = 0.01) were found. Splitting our population (O + N) into two groups, according to the median value of mRNA expression miR-33a levels (0.701), irrespective of the presence or absence of obesity, we observed that those having a higher expression of miR-33a were more frequently obese (87.5% vs. 12.5%; p < 0.0001) and had significantly increased values of auxological and metabolic parameters. Exosomes extracted from plasma of N and O carried miR-33a, and its expression was lower in O (p = 0.026). No correlations with metabolic parameters were observed. Conclusion: While exosome miR-33a does not provide any advantage, circulating miR-33a can provide important indications in an initial phase of metabolic dysfunction, stratifying obese adolescents at higher cardiometabolic risk. |
format | Online Article Text |
id | pubmed-10452681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104526812023-08-26 Circulating and Exosomal microRNA-33 in Childhood Obesity Cabiati, Manuela Guiducci, Letizia Randazzo, Emioli Casieri, Valentina Federico, Giovanni Del Ry, Silvia Biomedicines Article Background: MicroRNA-33 may control a wide range of different metabolic functions. Methods: This study aims to assess the miR-33a circulating profile in normal-weight (N = 20) and obese (O = 30) adolescents and to correlate its expression levels to their metabolic parameters. In a subset of subjects, we compared circulating miR-33a with exosomal miR-33a. Results: Metabolic parameters were altered in O, with initial hyperinsulinemia. Circulating miR-33a was significantly higher in O than in N (p = 0.0002). Significant correlations between miR-33a and auxological and metabolic indices (Insulin p = 0.01; Cholesterol p = 0.01; LDL p = 0.01; HbA1c p = 0.01) were found. Splitting our population (O + N) into two groups, according to the median value of mRNA expression miR-33a levels (0.701), irrespective of the presence or absence of obesity, we observed that those having a higher expression of miR-33a were more frequently obese (87.5% vs. 12.5%; p < 0.0001) and had significantly increased values of auxological and metabolic parameters. Exosomes extracted from plasma of N and O carried miR-33a, and its expression was lower in O (p = 0.026). No correlations with metabolic parameters were observed. Conclusion: While exosome miR-33a does not provide any advantage, circulating miR-33a can provide important indications in an initial phase of metabolic dysfunction, stratifying obese adolescents at higher cardiometabolic risk. MDPI 2023-08-18 /pmc/articles/PMC10452681/ /pubmed/37626791 http://dx.doi.org/10.3390/biomedicines11082295 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cabiati, Manuela Guiducci, Letizia Randazzo, Emioli Casieri, Valentina Federico, Giovanni Del Ry, Silvia Circulating and Exosomal microRNA-33 in Childhood Obesity |
title | Circulating and Exosomal microRNA-33 in Childhood Obesity |
title_full | Circulating and Exosomal microRNA-33 in Childhood Obesity |
title_fullStr | Circulating and Exosomal microRNA-33 in Childhood Obesity |
title_full_unstemmed | Circulating and Exosomal microRNA-33 in Childhood Obesity |
title_short | Circulating and Exosomal microRNA-33 in Childhood Obesity |
title_sort | circulating and exosomal microrna-33 in childhood obesity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452681/ https://www.ncbi.nlm.nih.gov/pubmed/37626791 http://dx.doi.org/10.3390/biomedicines11082295 |
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