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Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors

SIMPLE SUMMARY: The study of whole-genome sequences, combined with advanced bioinformatic tools, has revolutionized our understanding of the evolutionary history of genes and genomes. Here, we have highlighted the conservation of a putative regulatory element in insect genes encoding for an importan...

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Autores principales: Lovero, Domenica, Porcelli, Damiano, Giordano, Luca, Lo Giudice, Claudio, Picardi, Ernesto, Pesole, Graziano, Pignataro, Eugenia, Palazzo, Antonio, Marsano, René Massimiliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452791/
https://www.ncbi.nlm.nih.gov/pubmed/37627011
http://dx.doi.org/10.3390/biology12081127
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author Lovero, Domenica
Porcelli, Damiano
Giordano, Luca
Lo Giudice, Claudio
Picardi, Ernesto
Pesole, Graziano
Pignataro, Eugenia
Palazzo, Antonio
Marsano, René Massimiliano
author_facet Lovero, Domenica
Porcelli, Damiano
Giordano, Luca
Lo Giudice, Claudio
Picardi, Ernesto
Pesole, Graziano
Pignataro, Eugenia
Palazzo, Antonio
Marsano, René Massimiliano
author_sort Lovero, Domenica
collection PubMed
description SIMPLE SUMMARY: The study of whole-genome sequences, combined with advanced bioinformatic tools, has revolutionized our understanding of the evolutionary history of genes and genomes. Here, we have highlighted the conservation of a putative regulatory element in insect genes encoding for an important enzymatic complex of the vacuolar and lysosomal compartments of the eukaryotic cell with storage and recycling functions, respectively. This study will enable future investigations aimed at understanding regulative circuits that generate cellular complexity. ABSTRACT: Gene and genome comparison represent an invaluable tool to identify evolutionarily conserved sequences with possible functional significance. In this work, we have analyzed orthologous genes encoding subunits and assembly factors of the V-ATPase complex, an important enzymatic complex of the vacuolar and lysosomal compartments of the eukaryotic cell with storage and recycling functions, respectively, as well as the main pump in the plasma membrane that energizes the epithelial transport in insects. This study involves 70 insect species belonging to eight insect orders. We highlighted the conservation of a short sequence in the genes encoding subunits of the V-ATPase complex and their assembly factors analyzed with respect to their exon-intron organization of those genes. This study offers the possibility to study ultra-conserved regulatory elements under an evolutionary perspective, with the aim of expanding our knowledge on the regulation of complex gene networks at the basis of organellar biogenesis and cellular organization.
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spelling pubmed-104527912023-08-26 Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors Lovero, Domenica Porcelli, Damiano Giordano, Luca Lo Giudice, Claudio Picardi, Ernesto Pesole, Graziano Pignataro, Eugenia Palazzo, Antonio Marsano, René Massimiliano Biology (Basel) Article SIMPLE SUMMARY: The study of whole-genome sequences, combined with advanced bioinformatic tools, has revolutionized our understanding of the evolutionary history of genes and genomes. Here, we have highlighted the conservation of a putative regulatory element in insect genes encoding for an important enzymatic complex of the vacuolar and lysosomal compartments of the eukaryotic cell with storage and recycling functions, respectively. This study will enable future investigations aimed at understanding regulative circuits that generate cellular complexity. ABSTRACT: Gene and genome comparison represent an invaluable tool to identify evolutionarily conserved sequences with possible functional significance. In this work, we have analyzed orthologous genes encoding subunits and assembly factors of the V-ATPase complex, an important enzymatic complex of the vacuolar and lysosomal compartments of the eukaryotic cell with storage and recycling functions, respectively, as well as the main pump in the plasma membrane that energizes the epithelial transport in insects. This study involves 70 insect species belonging to eight insect orders. We highlighted the conservation of a short sequence in the genes encoding subunits of the V-ATPase complex and their assembly factors analyzed with respect to their exon-intron organization of those genes. This study offers the possibility to study ultra-conserved regulatory elements under an evolutionary perspective, with the aim of expanding our knowledge on the regulation of complex gene networks at the basis of organellar biogenesis and cellular organization. MDPI 2023-08-13 /pmc/articles/PMC10452791/ /pubmed/37627011 http://dx.doi.org/10.3390/biology12081127 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lovero, Domenica
Porcelli, Damiano
Giordano, Luca
Lo Giudice, Claudio
Picardi, Ernesto
Pesole, Graziano
Pignataro, Eugenia
Palazzo, Antonio
Marsano, René Massimiliano
Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title_full Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title_fullStr Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title_full_unstemmed Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title_short Structural and Comparative Analyses of Insects Suggest the Presence of an Ultra-Conserved Regulatory Element of the Genes Encoding Vacuolar-Type ATPase Subunits and Assembly Factors
title_sort structural and comparative analyses of insects suggest the presence of an ultra-conserved regulatory element of the genes encoding vacuolar-type atpase subunits and assembly factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10452791/
https://www.ncbi.nlm.nih.gov/pubmed/37627011
http://dx.doi.org/10.3390/biology12081127
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