Cargando…
Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids
Serum amyloid A (SAA) proteins belong to a family of acute-phase reactants, playing an integral role in defending the organism from pathological damage. Despite a wealth of data on the regulation of SAA transcripts in teleosts, there is only limited information on these proteins’ abundance in fish....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453338/ https://www.ncbi.nlm.nih.gov/pubmed/37626907 http://dx.doi.org/10.3390/cells12162097 |
_version_ | 1785095911584563200 |
---|---|
author | Buks, Ralfs Alnabulsi, Abdo Zindrili, Rodanthi Alnabulsi, Ayham Wang, Alex Wang, Tiehui Martin, Samuel A. M. |
author_facet | Buks, Ralfs Alnabulsi, Abdo Zindrili, Rodanthi Alnabulsi, Ayham Wang, Alex Wang, Tiehui Martin, Samuel A. M. |
author_sort | Buks, Ralfs |
collection | PubMed |
description | Serum amyloid A (SAA) proteins belong to a family of acute-phase reactants, playing an integral role in defending the organism from pathological damage. Despite a wealth of data on the regulation of SAA transcripts in teleosts, there is only limited information on these proteins’ abundance in fish. The aim of this study is to characterise SAA protein levels in salmonids using a newly developed antibody specific to salmonid SAA. The salmonid SAA antibody detected SAA and accurately discriminated between stimulated and control specimens from rainbow trout macrophage cell line (RTS-11) in vitro, as well as rainbow trout challenged with Aeromonas salmonicida- or flagellin-stimulated Atlantic salmon in vivo. The presence of SAA protein was analysed in RTS-11 cell line supernatants, liver, and spleen samples using ELISA, immunoblotting, and immunohistochemistry. This study is the first to characterise SAA protein levels in salmonids in vivo and in vitro. The newly developed salmonid SAA antibody was able to discriminate between stimulated and unstimulated specimens, showing that it can be used to study the acute-phase response in salmonids with the potential to be further developed into assays to monitor and evaluate health in wild and farmed fish. |
format | Online Article Text |
id | pubmed-10453338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104533382023-08-26 Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids Buks, Ralfs Alnabulsi, Abdo Zindrili, Rodanthi Alnabulsi, Ayham Wang, Alex Wang, Tiehui Martin, Samuel A. M. Cells Article Serum amyloid A (SAA) proteins belong to a family of acute-phase reactants, playing an integral role in defending the organism from pathological damage. Despite a wealth of data on the regulation of SAA transcripts in teleosts, there is only limited information on these proteins’ abundance in fish. The aim of this study is to characterise SAA protein levels in salmonids using a newly developed antibody specific to salmonid SAA. The salmonid SAA antibody detected SAA and accurately discriminated between stimulated and control specimens from rainbow trout macrophage cell line (RTS-11) in vitro, as well as rainbow trout challenged with Aeromonas salmonicida- or flagellin-stimulated Atlantic salmon in vivo. The presence of SAA protein was analysed in RTS-11 cell line supernatants, liver, and spleen samples using ELISA, immunoblotting, and immunohistochemistry. This study is the first to characterise SAA protein levels in salmonids in vivo and in vitro. The newly developed salmonid SAA antibody was able to discriminate between stimulated and unstimulated specimens, showing that it can be used to study the acute-phase response in salmonids with the potential to be further developed into assays to monitor and evaluate health in wild and farmed fish. MDPI 2023-08-19 /pmc/articles/PMC10453338/ /pubmed/37626907 http://dx.doi.org/10.3390/cells12162097 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Buks, Ralfs Alnabulsi, Abdo Zindrili, Rodanthi Alnabulsi, Ayham Wang, Alex Wang, Tiehui Martin, Samuel A. M. Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title | Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title_full | Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title_fullStr | Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title_full_unstemmed | Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title_short | Catch of the Day: New Serum Amyloid A (SAA) Antibody Is a Valuable Tool to Study Fish Health in Salmonids |
title_sort | catch of the day: new serum amyloid a (saa) antibody is a valuable tool to study fish health in salmonids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453338/ https://www.ncbi.nlm.nih.gov/pubmed/37626907 http://dx.doi.org/10.3390/cells12162097 |
work_keys_str_mv | AT buksralfs catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT alnabulsiabdo catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT zindrilirodanthi catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT alnabulsiayham catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT wangalex catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT wangtiehui catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids AT martinsamuelam catchofthedaynewserumamyloidasaaantibodyisavaluabletooltostudyfishhealthinsalmonids |