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Advances in Genetic Tools and Their Application in Streptococcus thermophilus

Streptococcus thermophilus is a traditional starter. Nowadays, key aspects of S. thermophilus physiology have been revealed concerning the phenotypic traits relevant for industrial applications, including sugar metabolism, protein hydrolysis, and the production of important metabolites that affect t...

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Autores principales: Zhao, Ruiting, Chen, Zouquan, Liang, Jie, Dou, Jiaxin, Guo, Fangyu, Xu, Zhenshang, Wang, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453384/
https://www.ncbi.nlm.nih.gov/pubmed/37628118
http://dx.doi.org/10.3390/foods12163119
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author Zhao, Ruiting
Chen, Zouquan
Liang, Jie
Dou, Jiaxin
Guo, Fangyu
Xu, Zhenshang
Wang, Ting
author_facet Zhao, Ruiting
Chen, Zouquan
Liang, Jie
Dou, Jiaxin
Guo, Fangyu
Xu, Zhenshang
Wang, Ting
author_sort Zhao, Ruiting
collection PubMed
description Streptococcus thermophilus is a traditional starter. Nowadays, key aspects of S. thermophilus physiology have been revealed concerning the phenotypic traits relevant for industrial applications, including sugar metabolism, protein hydrolysis, and the production of important metabolites that affect the sensory properties of fermented foods as well as the original cooperation with Lactobacillus delbrueckii subsp. bulgaricus. Moreover, significant advances have been made in the synthetic biology toolbox of S. thermophilus based on technological advances in the genome and its sequencing and synthesis. In this review, we discuss the recently developed toolbox for S. thermophilus, including gene expression toolsets (promoters, terminators, plasmids, etc.) and genome editing tools. It can be used for both functionalized foods and therapeutic molecules for consumers. The availability of new molecular tools, including the genome editing toolbox, has facilitated the engineering of physiological studies of S. thermophilus and the generation of strains with improved technical and functional characteristics.
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spelling pubmed-104533842023-08-26 Advances in Genetic Tools and Their Application in Streptococcus thermophilus Zhao, Ruiting Chen, Zouquan Liang, Jie Dou, Jiaxin Guo, Fangyu Xu, Zhenshang Wang, Ting Foods Review Streptococcus thermophilus is a traditional starter. Nowadays, key aspects of S. thermophilus physiology have been revealed concerning the phenotypic traits relevant for industrial applications, including sugar metabolism, protein hydrolysis, and the production of important metabolites that affect the sensory properties of fermented foods as well as the original cooperation with Lactobacillus delbrueckii subsp. bulgaricus. Moreover, significant advances have been made in the synthetic biology toolbox of S. thermophilus based on technological advances in the genome and its sequencing and synthesis. In this review, we discuss the recently developed toolbox for S. thermophilus, including gene expression toolsets (promoters, terminators, plasmids, etc.) and genome editing tools. It can be used for both functionalized foods and therapeutic molecules for consumers. The availability of new molecular tools, including the genome editing toolbox, has facilitated the engineering of physiological studies of S. thermophilus and the generation of strains with improved technical and functional characteristics. MDPI 2023-08-19 /pmc/articles/PMC10453384/ /pubmed/37628118 http://dx.doi.org/10.3390/foods12163119 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhao, Ruiting
Chen, Zouquan
Liang, Jie
Dou, Jiaxin
Guo, Fangyu
Xu, Zhenshang
Wang, Ting
Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title_full Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title_fullStr Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title_full_unstemmed Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title_short Advances in Genetic Tools and Their Application in Streptococcus thermophilus
title_sort advances in genetic tools and their application in streptococcus thermophilus
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453384/
https://www.ncbi.nlm.nih.gov/pubmed/37628118
http://dx.doi.org/10.3390/foods12163119
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