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Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces

The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from...

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Autores principales: Minor, Martha, Sabillón, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453762/
https://www.ncbi.nlm.nih.gov/pubmed/37628070
http://dx.doi.org/10.3390/foods12163072
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author Minor, Martha
Sabillón, Luis
author_facet Minor, Martha
Sabillón, Luis
author_sort Minor, Martha
collection PubMed
description The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from 221 to 1107 J/cm(2). The efficacy of these treatments to inactivate E. coli O157:H7 dry cells was evaluated on clean and soiled stainless steel and cast-iron surfaces. On clean metal surfaces, the 405 and 460 nm LED treatment with a 221 J/cm(2) dose resulted in E. coli reductions ranging from 2.0 to 4.1 log CFU/cm(2). Increasing the treatment energy dose to 665 J/cm(2) caused further significant reductions (>8 log CFU/cm(2)) in the E. coli population. LED treatments triggered a significant production of intracellular reactive oxygen species (ROS) in E. coli cells, as well as a significant temperature increase on metal surfaces. In the presence of organic matter, intracellular ROS generation in E. coli cells dropped significantly, and treatments with higher energy doses (>700 J/cm(2)) were required to uphold antimicrobial effectiveness. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. This study showed that LEDs emitting monochromatic blue light at UHI levels may serve as a viable and time-effective method for surface decontamination in dry food processing environments.
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spelling pubmed-104537622023-08-26 Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces Minor, Martha Sabillón, Luis Foods Article The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from 221 to 1107 J/cm(2). The efficacy of these treatments to inactivate E. coli O157:H7 dry cells was evaluated on clean and soiled stainless steel and cast-iron surfaces. On clean metal surfaces, the 405 and 460 nm LED treatment with a 221 J/cm(2) dose resulted in E. coli reductions ranging from 2.0 to 4.1 log CFU/cm(2). Increasing the treatment energy dose to 665 J/cm(2) caused further significant reductions (>8 log CFU/cm(2)) in the E. coli population. LED treatments triggered a significant production of intracellular reactive oxygen species (ROS) in E. coli cells, as well as a significant temperature increase on metal surfaces. In the presence of organic matter, intracellular ROS generation in E. coli cells dropped significantly, and treatments with higher energy doses (>700 J/cm(2)) were required to uphold antimicrobial effectiveness. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. This study showed that LEDs emitting monochromatic blue light at UHI levels may serve as a viable and time-effective method for surface decontamination in dry food processing environments. MDPI 2023-08-16 /pmc/articles/PMC10453762/ /pubmed/37628070 http://dx.doi.org/10.3390/foods12163072 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minor, Martha
Sabillón, Luis
Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title_full Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title_fullStr Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title_full_unstemmed Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title_short Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
title_sort effectiveness of ultra-high irradiance blue light-emitting diodes in inactivating escherichia coli o157:h7 on dry stainless steel and cast-iron surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453762/
https://www.ncbi.nlm.nih.gov/pubmed/37628070
http://dx.doi.org/10.3390/foods12163072
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