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Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces
The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453762/ https://www.ncbi.nlm.nih.gov/pubmed/37628070 http://dx.doi.org/10.3390/foods12163072 |
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author | Minor, Martha Sabillón, Luis |
author_facet | Minor, Martha Sabillón, Luis |
author_sort | Minor, Martha |
collection | PubMed |
description | The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from 221 to 1107 J/cm(2). The efficacy of these treatments to inactivate E. coli O157:H7 dry cells was evaluated on clean and soiled stainless steel and cast-iron surfaces. On clean metal surfaces, the 405 and 460 nm LED treatment with a 221 J/cm(2) dose resulted in E. coli reductions ranging from 2.0 to 4.1 log CFU/cm(2). Increasing the treatment energy dose to 665 J/cm(2) caused further significant reductions (>8 log CFU/cm(2)) in the E. coli population. LED treatments triggered a significant production of intracellular reactive oxygen species (ROS) in E. coli cells, as well as a significant temperature increase on metal surfaces. In the presence of organic matter, intracellular ROS generation in E. coli cells dropped significantly, and treatments with higher energy doses (>700 J/cm(2)) were required to uphold antimicrobial effectiveness. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. This study showed that LEDs emitting monochromatic blue light at UHI levels may serve as a viable and time-effective method for surface decontamination in dry food processing environments. |
format | Online Article Text |
id | pubmed-10453762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104537622023-08-26 Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces Minor, Martha Sabillón, Luis Foods Article The use of blue light-emitting diodes (LEDs) is emerging as a promising dry decontamination method. In the present study, LEDs emitting ultra-high irradiance (UHI) density at 405 nm (842 mW/cm(2)) and 460 nm (615 mW/cm(2)) were used to deliver high-intensity photoinactivation treatments ranging from 221 to 1107 J/cm(2). The efficacy of these treatments to inactivate E. coli O157:H7 dry cells was evaluated on clean and soiled stainless steel and cast-iron surfaces. On clean metal surfaces, the 405 and 460 nm LED treatment with a 221 J/cm(2) dose resulted in E. coli reductions ranging from 2.0 to 4.1 log CFU/cm(2). Increasing the treatment energy dose to 665 J/cm(2) caused further significant reductions (>8 log CFU/cm(2)) in the E. coli population. LED treatments triggered a significant production of intracellular reactive oxygen species (ROS) in E. coli cells, as well as a significant temperature increase on metal surfaces. In the presence of organic matter, intracellular ROS generation in E. coli cells dropped significantly, and treatments with higher energy doses (>700 J/cm(2)) were required to uphold antimicrobial effectiveness. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. This study showed that LEDs emitting monochromatic blue light at UHI levels may serve as a viable and time-effective method for surface decontamination in dry food processing environments. MDPI 2023-08-16 /pmc/articles/PMC10453762/ /pubmed/37628070 http://dx.doi.org/10.3390/foods12163072 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Minor, Martha Sabillón, Luis Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title | Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title_full | Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title_fullStr | Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title_full_unstemmed | Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title_short | Effectiveness of Ultra-High Irradiance Blue Light-Emitting Diodes in Inactivating Escherichia coli O157:H7 on Dry Stainless Steel and Cast-Iron Surfaces |
title_sort | effectiveness of ultra-high irradiance blue light-emitting diodes in inactivating escherichia coli o157:h7 on dry stainless steel and cast-iron surfaces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453762/ https://www.ncbi.nlm.nih.gov/pubmed/37628070 http://dx.doi.org/10.3390/foods12163072 |
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