The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro

Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor...

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Autores principales: Chapa-Dubocq, Xavier R., Rodríguez-Graciani, Keishla M., García-Báez, Jorge, Vadovsky, Alyssa, Bazil, Jason N., Javadov, Sabzali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453793/
https://www.ncbi.nlm.nih.gov/pubmed/37626827
http://dx.doi.org/10.3390/cells12162017
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author Chapa-Dubocq, Xavier R.
Rodríguez-Graciani, Keishla M.
García-Báez, Jorge
Vadovsky, Alyssa
Bazil, Jason N.
Javadov, Sabzali
author_facet Chapa-Dubocq, Xavier R.
Rodríguez-Graciani, Keishla M.
García-Báez, Jorge
Vadovsky, Alyssa
Bazil, Jason N.
Javadov, Sabzali
author_sort Chapa-Dubocq, Xavier R.
collection PubMed
description Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor) and TPEN (an OMA1 inhibitor). The rate of ADP-stimulated respiration was found diminished by both inhibitors, and they did not prevent Ca(2+)-induced mitochondrial respiratory dysfunction, membrane depolarization, or swelling. L-OPA1 cleavage was stimulated at state 3 respiration; therefore, our data suggest that L-OPA1 cleavage produces S-OPA1 to maintain mitochondrial bioenergetics in response to stress.
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spelling pubmed-104537932023-08-26 The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro Chapa-Dubocq, Xavier R. Rodríguez-Graciani, Keishla M. García-Báez, Jorge Vadovsky, Alyssa Bazil, Jason N. Javadov, Sabzali Cells Article Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor) and TPEN (an OMA1 inhibitor). The rate of ADP-stimulated respiration was found diminished by both inhibitors, and they did not prevent Ca(2+)-induced mitochondrial respiratory dysfunction, membrane depolarization, or swelling. L-OPA1 cleavage was stimulated at state 3 respiration; therefore, our data suggest that L-OPA1 cleavage produces S-OPA1 to maintain mitochondrial bioenergetics in response to stress. MDPI 2023-08-08 /pmc/articles/PMC10453793/ /pubmed/37626827 http://dx.doi.org/10.3390/cells12162017 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chapa-Dubocq, Xavier R.
Rodríguez-Graciani, Keishla M.
García-Báez, Jorge
Vadovsky, Alyssa
Bazil, Jason N.
Javadov, Sabzali
The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title_full The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title_fullStr The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title_full_unstemmed The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title_short The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro
title_sort role of swelling in the regulation of opa1-mediated mitochondrial function in the heart in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453793/
https://www.ncbi.nlm.nih.gov/pubmed/37626827
http://dx.doi.org/10.3390/cells12162017
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