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TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm

Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases...

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Autores principales: Wang, Ziqi, Fang, Kailun, Wan, Yanling, Yin, Yingying, Li, Mengjing, Xu, Ke, Li, Tongtong, Cao, Yongzhi, Lv, Yue, Lu, Gang, Liu, Hongbin, Huang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453820/
https://www.ncbi.nlm.nih.gov/pubmed/37626901
http://dx.doi.org/10.3390/cells12162091
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author Wang, Ziqi
Fang, Kailun
Wan, Yanling
Yin, Yingying
Li, Mengjing
Xu, Ke
Li, Tongtong
Cao, Yongzhi
Lv, Yue
Lu, Gang
Liu, Hongbin
Huang, Tao
author_facet Wang, Ziqi
Fang, Kailun
Wan, Yanling
Yin, Yingying
Li, Mengjing
Xu, Ke
Li, Tongtong
Cao, Yongzhi
Lv, Yue
Lu, Gang
Liu, Hongbin
Huang, Tao
author_sort Wang, Ziqi
collection PubMed
description Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases related to the flagellum, the underlying mechanisms that control sperm movement are not yet fully understood. In this study, we reveal that the tetratricopeptide repeat protein 6 (Ttc6) gene, expressed mainly in the testes, plays a crucial role in maintaining male fertility in mice. We further demonstrate that the knockout of Ttc6 in mice results in decreased sperm motility and induces an abnormal circular swimming pattern, consequently leading to male subfertility. Morphological analysis showed an atypical hairpin-like appearance of the spermatozoa, and ultrastructural studies showed unsheathed flagella at the juncture between the midpiece and principal piece. Collectively, these findings suggest that TTC6 plays an essential role in maintaining the stability of the annulus region of the sperm flagellum, thus ensuring the swift and directed motion of sperm.
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spelling pubmed-104538202023-08-26 TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm Wang, Ziqi Fang, Kailun Wan, Yanling Yin, Yingying Li, Mengjing Xu, Ke Li, Tongtong Cao, Yongzhi Lv, Yue Lu, Gang Liu, Hongbin Huang, Tao Cells Article Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases related to the flagellum, the underlying mechanisms that control sperm movement are not yet fully understood. In this study, we reveal that the tetratricopeptide repeat protein 6 (Ttc6) gene, expressed mainly in the testes, plays a crucial role in maintaining male fertility in mice. We further demonstrate that the knockout of Ttc6 in mice results in decreased sperm motility and induces an abnormal circular swimming pattern, consequently leading to male subfertility. Morphological analysis showed an atypical hairpin-like appearance of the spermatozoa, and ultrastructural studies showed unsheathed flagella at the juncture between the midpiece and principal piece. Collectively, these findings suggest that TTC6 plays an essential role in maintaining the stability of the annulus region of the sperm flagellum, thus ensuring the swift and directed motion of sperm. MDPI 2023-08-18 /pmc/articles/PMC10453820/ /pubmed/37626901 http://dx.doi.org/10.3390/cells12162091 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Ziqi
Fang, Kailun
Wan, Yanling
Yin, Yingying
Li, Mengjing
Xu, Ke
Li, Tongtong
Cao, Yongzhi
Lv, Yue
Lu, Gang
Liu, Hongbin
Huang, Tao
TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title_full TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title_fullStr TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title_full_unstemmed TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title_short TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
title_sort ttc6-mediated stabilization of the flagellum annulus ensures the rapid and directed motion of sperm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453820/
https://www.ncbi.nlm.nih.gov/pubmed/37626901
http://dx.doi.org/10.3390/cells12162091
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