Cargando…
TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm
Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453820/ https://www.ncbi.nlm.nih.gov/pubmed/37626901 http://dx.doi.org/10.3390/cells12162091 |
_version_ | 1785096036436410368 |
---|---|
author | Wang, Ziqi Fang, Kailun Wan, Yanling Yin, Yingying Li, Mengjing Xu, Ke Li, Tongtong Cao, Yongzhi Lv, Yue Lu, Gang Liu, Hongbin Huang, Tao |
author_facet | Wang, Ziqi Fang, Kailun Wan, Yanling Yin, Yingying Li, Mengjing Xu, Ke Li, Tongtong Cao, Yongzhi Lv, Yue Lu, Gang Liu, Hongbin Huang, Tao |
author_sort | Wang, Ziqi |
collection | PubMed |
description | Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases related to the flagellum, the underlying mechanisms that control sperm movement are not yet fully understood. In this study, we reveal that the tetratricopeptide repeat protein 6 (Ttc6) gene, expressed mainly in the testes, plays a crucial role in maintaining male fertility in mice. We further demonstrate that the knockout of Ttc6 in mice results in decreased sperm motility and induces an abnormal circular swimming pattern, consequently leading to male subfertility. Morphological analysis showed an atypical hairpin-like appearance of the spermatozoa, and ultrastructural studies showed unsheathed flagella at the juncture between the midpiece and principal piece. Collectively, these findings suggest that TTC6 plays an essential role in maintaining the stability of the annulus region of the sperm flagellum, thus ensuring the swift and directed motion of sperm. |
format | Online Article Text |
id | pubmed-10453820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104538202023-08-26 TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm Wang, Ziqi Fang, Kailun Wan, Yanling Yin, Yingying Li, Mengjing Xu, Ke Li, Tongtong Cao, Yongzhi Lv, Yue Lu, Gang Liu, Hongbin Huang, Tao Cells Article Sperm motility and structural integrity are essential for successful fertilization in vivo, and any hindrance of the correct assembly of the axoneme and peri-axonemal structures in the sperm flagellum can lead to fertility problems. While there has been considerable advancement in studying diseases related to the flagellum, the underlying mechanisms that control sperm movement are not yet fully understood. In this study, we reveal that the tetratricopeptide repeat protein 6 (Ttc6) gene, expressed mainly in the testes, plays a crucial role in maintaining male fertility in mice. We further demonstrate that the knockout of Ttc6 in mice results in decreased sperm motility and induces an abnormal circular swimming pattern, consequently leading to male subfertility. Morphological analysis showed an atypical hairpin-like appearance of the spermatozoa, and ultrastructural studies showed unsheathed flagella at the juncture between the midpiece and principal piece. Collectively, these findings suggest that TTC6 plays an essential role in maintaining the stability of the annulus region of the sperm flagellum, thus ensuring the swift and directed motion of sperm. MDPI 2023-08-18 /pmc/articles/PMC10453820/ /pubmed/37626901 http://dx.doi.org/10.3390/cells12162091 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Ziqi Fang, Kailun Wan, Yanling Yin, Yingying Li, Mengjing Xu, Ke Li, Tongtong Cao, Yongzhi Lv, Yue Lu, Gang Liu, Hongbin Huang, Tao TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title | TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title_full | TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title_fullStr | TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title_full_unstemmed | TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title_short | TTC6-Mediated Stabilization of the Flagellum Annulus Ensures the Rapid and Directed Motion of Sperm |
title_sort | ttc6-mediated stabilization of the flagellum annulus ensures the rapid and directed motion of sperm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453820/ https://www.ncbi.nlm.nih.gov/pubmed/37626901 http://dx.doi.org/10.3390/cells12162091 |
work_keys_str_mv | AT wangziqi ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT fangkailun ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT wanyanling ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT yinyingying ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT limengjing ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT xuke ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT litongtong ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT caoyongzhi ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT lvyue ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT lugang ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT liuhongbin ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm AT huangtao ttc6mediatedstabilizationoftheflagellumannulusensurestherapidanddirectedmotionofsperm |