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Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study

The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem ma...

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Autores principales: Grochowski, Emil Tomasz, Pietrowska, Karolina, Godlewski, Adrian, Gosk, Wioleta, Buczynska, Angelika, Wojnar, Malgorzata, Konopinska, Joanna, Kretowski, Adam, Ciborowski, Michal, Dmuchowska, Diana Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454064/
https://www.ncbi.nlm.nih.gov/pubmed/37628855
http://dx.doi.org/10.3390/ijms241612671
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author Grochowski, Emil Tomasz
Pietrowska, Karolina
Godlewski, Adrian
Gosk, Wioleta
Buczynska, Angelika
Wojnar, Malgorzata
Konopinska, Joanna
Kretowski, Adam
Ciborowski, Michal
Dmuchowska, Diana Anna
author_facet Grochowski, Emil Tomasz
Pietrowska, Karolina
Godlewski, Adrian
Gosk, Wioleta
Buczynska, Angelika
Wojnar, Malgorzata
Konopinska, Joanna
Kretowski, Adam
Ciborowski, Michal
Dmuchowska, Diana Anna
author_sort Grochowski, Emil Tomasz
collection PubMed
description The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ(®) p180 kit. Out of 188 metabolites targeted by the kit, 41 and 133 were detected in >80% of AH and serum samples, respectively. Statistical analysis performed to indicate metabolites differentiating diabetic and nondiabetic patients showed 8 and 20 significant metabolites in AH and serum, respectively. Pathway analysis performed for significant metabolites revealed that galactose metabolism is mostly affected in the AH, while arginine biosynthesis is mostly affected in the serum. Among metabolites that differentiate diabetic and nondiabetic patients, arginine was the only metabolite common to both serum and AH samples, as well as the only one with a decreased concentration in both body fluids of diabetic patients. Concentrations of the rest were elevated in AH and lowered in serum. This may suggest different mechanisms of diabetes-related dysregulation of the local metabolism in the eye in comparison to systemic changes observed in the blood.
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spelling pubmed-104540642023-08-26 Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study Grochowski, Emil Tomasz Pietrowska, Karolina Godlewski, Adrian Gosk, Wioleta Buczynska, Angelika Wojnar, Malgorzata Konopinska, Joanna Kretowski, Adam Ciborowski, Michal Dmuchowska, Diana Anna Int J Mol Sci Article The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ(®) p180 kit. Out of 188 metabolites targeted by the kit, 41 and 133 were detected in >80% of AH and serum samples, respectively. Statistical analysis performed to indicate metabolites differentiating diabetic and nondiabetic patients showed 8 and 20 significant metabolites in AH and serum, respectively. Pathway analysis performed for significant metabolites revealed that galactose metabolism is mostly affected in the AH, while arginine biosynthesis is mostly affected in the serum. Among metabolites that differentiate diabetic and nondiabetic patients, arginine was the only metabolite common to both serum and AH samples, as well as the only one with a decreased concentration in both body fluids of diabetic patients. Concentrations of the rest were elevated in AH and lowered in serum. This may suggest different mechanisms of diabetes-related dysregulation of the local metabolism in the eye in comparison to systemic changes observed in the blood. MDPI 2023-08-11 /pmc/articles/PMC10454064/ /pubmed/37628855 http://dx.doi.org/10.3390/ijms241612671 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grochowski, Emil Tomasz
Pietrowska, Karolina
Godlewski, Adrian
Gosk, Wioleta
Buczynska, Angelika
Wojnar, Malgorzata
Konopinska, Joanna
Kretowski, Adam
Ciborowski, Michal
Dmuchowska, Diana Anna
Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title_full Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title_fullStr Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title_full_unstemmed Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title_short Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery—A Targeted and Quantitative Metabolomics Study
title_sort simultaneous comparison of aqueous humor and serum metabolic profiles of diabetic and nondiabetic patients undergoing cataract surgery—a targeted and quantitative metabolomics study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454064/
https://www.ncbi.nlm.nih.gov/pubmed/37628855
http://dx.doi.org/10.3390/ijms241612671
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