CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis

Since the discovery of the CRISPR-Cas engineering system in 2012, several approaches for using this innovative molecular tool in therapeutic strategies and even diagnosis have been investigated. The use of this tool requires a global approach to DNA damage processes and repair systems in cells. The...

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Autores principales: Sauvagère, Stéphane, Siatka, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454384/
https://www.ncbi.nlm.nih.gov/pubmed/37628594
http://dx.doi.org/10.3390/genes14081542
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author Sauvagère, Stéphane
Siatka, Christian
author_facet Sauvagère, Stéphane
Siatka, Christian
author_sort Sauvagère, Stéphane
collection PubMed
description Since the discovery of the CRISPR-Cas engineering system in 2012, several approaches for using this innovative molecular tool in therapeutic strategies and even diagnosis have been investigated. The use of this tool requires a global approach to DNA damage processes and repair systems in cells. The diversity in the functions of various Cas proteins allows for the use of this technology in clinical applications and trials. Wide variants of Cas12 and Cas13 are exploited using the collateral effect in many diagnostic applications. Even though this tool is well known, its use still raises real-world ethical and regulatory questions.
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spelling pubmed-104543842023-08-26 CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis Sauvagère, Stéphane Siatka, Christian Genes (Basel) Review Since the discovery of the CRISPR-Cas engineering system in 2012, several approaches for using this innovative molecular tool in therapeutic strategies and even diagnosis have been investigated. The use of this tool requires a global approach to DNA damage processes and repair systems in cells. The diversity in the functions of various Cas proteins allows for the use of this technology in clinical applications and trials. Wide variants of Cas12 and Cas13 are exploited using the collateral effect in many diagnostic applications. Even though this tool is well known, its use still raises real-world ethical and regulatory questions. MDPI 2023-07-27 /pmc/articles/PMC10454384/ /pubmed/37628594 http://dx.doi.org/10.3390/genes14081542 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sauvagère, Stéphane
Siatka, Christian
CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title_full CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title_fullStr CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title_full_unstemmed CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title_short CRISPR-Cas: ‘The Multipurpose Molecular Tool’ for Gene Therapy and Diagnosis
title_sort crispr-cas: ‘the multipurpose molecular tool’ for gene therapy and diagnosis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454384/
https://www.ncbi.nlm.nih.gov/pubmed/37628594
http://dx.doi.org/10.3390/genes14081542
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