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QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy
Traditional otic drug delivery methods lack controlled release capabilities, making reverse gelatination gels a promising alternative. Reverse gelatination gels are colloidal systems that transition from a sol to a gel phase at the target site, providing controlled drug release over an extended peri...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454480/ https://www.ncbi.nlm.nih.gov/pubmed/37623112 http://dx.doi.org/10.3390/gels9080657 |
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author | Budhori, Amit Tiwari, Abhishek Tiwari, Varsha Sharma, Ajay Kumar, Manish Gautam, Girendra Virmani, Tarun Kumar, Girish Alhalmi, Abdulsalam Noman, Omar Mohammed Hasson, Sidgi Mothana, Ramzi A. |
author_facet | Budhori, Amit Tiwari, Abhishek Tiwari, Varsha Sharma, Ajay Kumar, Manish Gautam, Girendra Virmani, Tarun Kumar, Girish Alhalmi, Abdulsalam Noman, Omar Mohammed Hasson, Sidgi Mothana, Ramzi A. |
author_sort | Budhori, Amit |
collection | PubMed |
description | Traditional otic drug delivery methods lack controlled release capabilities, making reverse gelatination gels a promising alternative. Reverse gelatination gels are colloidal systems that transition from a sol to a gel phase at the target site, providing controlled drug release over an extended period. Thermosensitive norfloxacin reverse gelatination gels were developed using a Quality by Design (QbD)-based optimization approach. The formulations were evaluated for their in vitro release profile, rheological behavior, visual appearance, pH, gelling time, and sol–gel transition temperature. The results show that the gelation temperatures of the formulations ranged from 33 to 37 °C, with gelling durations between 35 and 90 s. The drug content in the formulations was uniform, with entrapment efficiency ranging from 55% to 95%. Among the formulations, F10 exhibited the most favorable properties and was selected for a stability study lasting 60 days. Ex-vivo release data demonstrate that the F10 formulation achieved 95.6percentage of drug release at 360 min. This study successfully developed thermosensitive norfloxacin reverse gelatination gels using a QbD-based optimization approach. The selected formulation, F10, exhibited desirable properties in terms of gelling temperature, drug content, and release profile. These gels hold potential for the controlled delivery of norfloxacin in the treatment of ear infections. |
format | Online Article Text |
id | pubmed-10454480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104544802023-08-26 QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy Budhori, Amit Tiwari, Abhishek Tiwari, Varsha Sharma, Ajay Kumar, Manish Gautam, Girendra Virmani, Tarun Kumar, Girish Alhalmi, Abdulsalam Noman, Omar Mohammed Hasson, Sidgi Mothana, Ramzi A. Gels Article Traditional otic drug delivery methods lack controlled release capabilities, making reverse gelatination gels a promising alternative. Reverse gelatination gels are colloidal systems that transition from a sol to a gel phase at the target site, providing controlled drug release over an extended period. Thermosensitive norfloxacin reverse gelatination gels were developed using a Quality by Design (QbD)-based optimization approach. The formulations were evaluated for their in vitro release profile, rheological behavior, visual appearance, pH, gelling time, and sol–gel transition temperature. The results show that the gelation temperatures of the formulations ranged from 33 to 37 °C, with gelling durations between 35 and 90 s. The drug content in the formulations was uniform, with entrapment efficiency ranging from 55% to 95%. Among the formulations, F10 exhibited the most favorable properties and was selected for a stability study lasting 60 days. Ex-vivo release data demonstrate that the F10 formulation achieved 95.6percentage of drug release at 360 min. This study successfully developed thermosensitive norfloxacin reverse gelatination gels using a QbD-based optimization approach. The selected formulation, F10, exhibited desirable properties in terms of gelling temperature, drug content, and release profile. These gels hold potential for the controlled delivery of norfloxacin in the treatment of ear infections. MDPI 2023-08-15 /pmc/articles/PMC10454480/ /pubmed/37623112 http://dx.doi.org/10.3390/gels9080657 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Budhori, Amit Tiwari, Abhishek Tiwari, Varsha Sharma, Ajay Kumar, Manish Gautam, Girendra Virmani, Tarun Kumar, Girish Alhalmi, Abdulsalam Noman, Omar Mohammed Hasson, Sidgi Mothana, Ramzi A. QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title | QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title_full | QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title_fullStr | QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title_full_unstemmed | QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title_short | QbD Design, Formulation, Optimization and Evaluation of Trans-Tympanic Reverse Gelatination Gel of Norfloxacin: Investigating Gene-Gene Interactions to Enhance Therapeutic Efficacy |
title_sort | qbd design, formulation, optimization and evaluation of trans-tympanic reverse gelatination gel of norfloxacin: investigating gene-gene interactions to enhance therapeutic efficacy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454480/ https://www.ncbi.nlm.nih.gov/pubmed/37623112 http://dx.doi.org/10.3390/gels9080657 |
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