Cargando…

Isolation of Hepatic and Adipose-Tissue-Derived Extracellular Vesicles Using Density Gradient Separation and Size Exclusion Chromatography

In recent years, the study of extracellular vesicles (EVs) in the context of various diseases has dramatically increased due to their diagnostic and therapeutic potential. Typically, EVs are isolated in vitro from the cell culture of primary cells or cell lines or from bodily fluids. However, these...

Descripción completa

Detalles Bibliográficos
Autores principales:	Martínez-Greene, Juan Alfonso, Gómez-Chavarín, Margarita, Ramos-Godínez, María del Pilar, Martínez-Martínez, Eduardo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454538/ https://www.ncbi.nlm.nih.gov/pubmed/37628890 http://dx.doi.org/10.3390/ijms241612704

Ejemplares similares

The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
por: Kaddour, Hussein, et al.
Publicado: (2021)

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion chromatography
por: Martínez‐Greene, Juan A., et al.
Publicado: (2021)

Single-step isolation of extracellular vesicles by size-exclusion chromatography
por: Böing, Anita N., et al.
Publicado: (2014)

Size-exclusion chromatography-based enrichment of extracellular vesicles from urine samples
por: Lozano-Ramos, Inés, et al.
Publicado: (2015)

Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study
por: Kong, Fang, et al.
Publicado: (2023)

Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer
por: Liu, Daniel S. K., et al.
Publicado: (2020)

High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
por: Bellotti, Cristina, et al.
Publicado: (2021)

Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
por: Foers, Andrew D., et al.
Publicado: (2018)

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography
por: Blans, Kristine, et al.
Publicado: (2017)

Detection and phenotyping of extracellular vesicles by size exclusion chromatography coupled with on-line fluorescence detection
por: Kitka, Diána, et al.
Publicado: (2019)

Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma
por: Yang, Yang, et al.
Publicado: (2021)

Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography
por: Guerreiro, Eduarda M., et al.
Publicado: (2018)

Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography
por: Corso, Giulia, et al.
Publicado: (2017)

Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation
por: Soares, Maria, et al.
Publicado: (2023)

Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents
por: Gámez-Valero, Ana, et al.
Publicado: (2016)

Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
por: Wei, Rui, et al.
Publicado: (2020)

Establishment of a simplified dichotomic size‐exclusion chromatography for isolating extracellular vesicles toward clinical applications
por: Guo, Jiahui, et al.
Publicado: (2021)

Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma
por: Jung, Jik-Han, et al.
Publicado: (2021)

Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
por: Krušić Alić, Vedrana, et al.
Publicado: (2022)

Strategies in size-exclusion chromatography /
Publicado: (1996)

The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
por: Davis, Chelsea N., et al.
Publicado: (2019)

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential
por: Takov, Kaloyan, et al.
Publicado: (2018)

Rapid Isolation of Extracellular Vesicles Using a Hydrophilic Porous Silica Gel-Based Size-Exclusion Chromatography Column
por: Yoshitake, Jun, et al.
Publicado: (2022)

Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease
por: Franco, Chiara, et al.
Publicado: (2023)

Combination of precipitation and size exclusion chromatography as an effective method for exosome like extracellular vesicle isolation from pericardial fluids
por: Chandrasekera, Dhananjie, et al.
Publicado: (2023)

Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies
por: Benedikter, Birke J., et al.
Publicado: (2017)

Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
por: Oeyen, Eline, et al.
Publicado: (2018)

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles
por: Visan, Kekoolani S., et al.
Publicado: (2022)

Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion chromatography and nano-flow cytometry
por: Lattmann, Evelyn, et al.
Publicado: (2023)

Liposome retention in size exclusion chromatography
por: Ruysschaert, Tristan, et al.
Publicado: (2005)

Chapter 5 Size-exclusion chromatography
por: Silberring, J., et al.
Publicado: (2004)

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma
por: Onódi, Zsófia, et al.
Publicado: (2018)

DENSITY GRADIENT SEPARATION OF SARCOTUBULAR VESICLES AND OTHER PARTICULATE CONSTITUENTS OF RABBIT MUSCLE
por: Seraydarian, K., et al.
Publicado: (1965)

Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation
por: Yuana, Yuana, et al.
Publicado: (2014)

Mass-Spectrometry Based Proteome Comparison of Extracellular Vesicle Isolation Methods: Comparison of ME-kit, Size-Exclusion Chromatography, and High-Speed Centrifugation
por: Askeland, Anders, et al.
Publicado: (2020)

A Deadenylase Assay by Size-Exclusion Chromatography
por: He, Guang-Jun, et al.
Publicado: (2012)

Metabolomics of small extracellular vesicles derived from isocitrate dehydrogenase 1-mutant HCT116 cells collected by semi-automated size exclusion chromatography
por: Hayasaka, Ryosuke, et al.
Publicado: (2023)

Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations
por: Iwai, Kazuya, et al.
Publicado: (2016)

A size-exclusion-based approach for purifying extracellular vesicles from human plasma
por: Vanderboom, Patrick M., et al.
Publicado: (2021)

Size-exclusion chromatography (SEC) of branched polymers and polysaccharides
por: Gaborieau, Marianne, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_i5d0o3snvu4t49f1oqmmsr364d