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Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans
While spaceflight is becoming more common than before, the hazards spaceflight and space microgravity pose to the human body remain relatively unexplored. Astronauts experience muscle atrophy after spaceflight, but the exact reasons for this and solutions are unknown. Here, we take advantage of the...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454569/ https://www.ncbi.nlm.nih.gov/pubmed/37628820 http://dx.doi.org/10.3390/ijms241612640 |
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author | Kim, Ban-seok Alcantara, Alfredo V. Moon, Je-Hyun Higashitani, Atsushi Higashitani, Nahoko Etheridge, Timothy Szewczyk, Nathaniel J. Deane, Colleen S. Gaffney, Christopher J. Higashibata, Akira Hashizume, Toko Yoon, Kyoung-hye Lee, Jin I. |
author_facet | Kim, Ban-seok Alcantara, Alfredo V. Moon, Je-Hyun Higashitani, Atsushi Higashitani, Nahoko Etheridge, Timothy Szewczyk, Nathaniel J. Deane, Colleen S. Gaffney, Christopher J. Higashibata, Akira Hashizume, Toko Yoon, Kyoung-hye Lee, Jin I. |
author_sort | Kim, Ban-seok |
collection | PubMed |
description | While spaceflight is becoming more common than before, the hazards spaceflight and space microgravity pose to the human body remain relatively unexplored. Astronauts experience muscle atrophy after spaceflight, but the exact reasons for this and solutions are unknown. Here, we take advantage of the nematode C. elegans to understand the effects of space microgravity on worm body wall muscle. We found that space microgravity induces muscle atrophy in C. elegans from two independent spaceflight missions. As a comparison to spaceflight-induced muscle atrophy, we assessed the effects of acute nutritional deprivation and muscle disuse on C. elegans muscle cells. We found that these two factors also induce muscle atrophy in the nematode. Finally, we identified clp-4, which encodes a calpain protease that promotes muscle atrophy. Mutants of clp-4 suppress starvation-induced muscle atrophy. Such comparative analyses of different factors causing muscle atrophy in C. elegans could provide a way to identify novel genetic factors regulating space microgravity-induced muscle atrophy. |
format | Online Article Text |
id | pubmed-10454569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104545692023-08-26 Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans Kim, Ban-seok Alcantara, Alfredo V. Moon, Je-Hyun Higashitani, Atsushi Higashitani, Nahoko Etheridge, Timothy Szewczyk, Nathaniel J. Deane, Colleen S. Gaffney, Christopher J. Higashibata, Akira Hashizume, Toko Yoon, Kyoung-hye Lee, Jin I. Int J Mol Sci Article While spaceflight is becoming more common than before, the hazards spaceflight and space microgravity pose to the human body remain relatively unexplored. Astronauts experience muscle atrophy after spaceflight, but the exact reasons for this and solutions are unknown. Here, we take advantage of the nematode C. elegans to understand the effects of space microgravity on worm body wall muscle. We found that space microgravity induces muscle atrophy in C. elegans from two independent spaceflight missions. As a comparison to spaceflight-induced muscle atrophy, we assessed the effects of acute nutritional deprivation and muscle disuse on C. elegans muscle cells. We found that these two factors also induce muscle atrophy in the nematode. Finally, we identified clp-4, which encodes a calpain protease that promotes muscle atrophy. Mutants of clp-4 suppress starvation-induced muscle atrophy. Such comparative analyses of different factors causing muscle atrophy in C. elegans could provide a way to identify novel genetic factors regulating space microgravity-induced muscle atrophy. MDPI 2023-08-10 /pmc/articles/PMC10454569/ /pubmed/37628820 http://dx.doi.org/10.3390/ijms241612640 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Ban-seok Alcantara, Alfredo V. Moon, Je-Hyun Higashitani, Atsushi Higashitani, Nahoko Etheridge, Timothy Szewczyk, Nathaniel J. Deane, Colleen S. Gaffney, Christopher J. Higashibata, Akira Hashizume, Toko Yoon, Kyoung-hye Lee, Jin I. Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title | Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title_full | Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title_fullStr | Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title_full_unstemmed | Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title_short | Comparative Analysis of Muscle Atrophy During Spaceflight, Nutritional Deficiency and Disuse in the Nematode Caenorhabditis elegans |
title_sort | comparative analysis of muscle atrophy during spaceflight, nutritional deficiency and disuse in the nematode caenorhabditis elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454569/ https://www.ncbi.nlm.nih.gov/pubmed/37628820 http://dx.doi.org/10.3390/ijms241612640 |
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