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HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama

Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocyte...

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Autores principales: Moreno, Ambar, González, Claudia, Góndola, Jessica, Chavarría, Oris, Ortiz, Alma, Castillo, Jorge, Castillo Mewa, Juan, Pascale, Juan Miguel, Martínez, Alexander Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454674/
https://www.ncbi.nlm.nih.gov/pubmed/37623048
http://dx.doi.org/10.3390/idr15040044
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author Moreno, Ambar
González, Claudia
Góndola, Jessica
Chavarría, Oris
Ortiz, Alma
Castillo, Jorge
Castillo Mewa, Juan
Pascale, Juan Miguel
Martínez, Alexander Augusto
author_facet Moreno, Ambar
González, Claudia
Góndola, Jessica
Chavarría, Oris
Ortiz, Alma
Castillo, Jorge
Castillo Mewa, Juan
Pascale, Juan Miguel
Martínez, Alexander Augusto
author_sort Moreno, Ambar
collection PubMed
description Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocytes and viral load tests to detect virologic failure. We sequenced the basal samples retrospectively using next-generation sequencing (NGS), looking for low-frequency mutations that had not been detected before using the Sanger sequencing method (SSM) and describing the response to ART. Twenty-two subjects developed virologic failure (VF), and thirteen of them had at least one drug-resistance mutation associated with Reverse Transcriptase Inhibitors (RTI) and Protease Inhibitors (PIs) at frequency levels ≤ 1%, not detected previously in their basal genotyping test. No resistance mutations were observed to Integrase Strand Transfer Inhibitors (INSTIs). We identified a possible cause of VF in ART-naïve subjects with low-frequency mutations detected. To our knowledge, this is the first evaluation of pre-existing drug resistance for HIV-1 minority variants carried out on ART-naïve people living with HIV/AIDS (PLWHA) by analyzing the HIV-1 pol gene using NGS in the country.
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spelling pubmed-104546742023-08-26 HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama Moreno, Ambar González, Claudia Góndola, Jessica Chavarría, Oris Ortiz, Alma Castillo, Jorge Castillo Mewa, Juan Pascale, Juan Miguel Martínez, Alexander Augusto Infect Dis Rep Brief Report Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocytes and viral load tests to detect virologic failure. We sequenced the basal samples retrospectively using next-generation sequencing (NGS), looking for low-frequency mutations that had not been detected before using the Sanger sequencing method (SSM) and describing the response to ART. Twenty-two subjects developed virologic failure (VF), and thirteen of them had at least one drug-resistance mutation associated with Reverse Transcriptase Inhibitors (RTI) and Protease Inhibitors (PIs) at frequency levels ≤ 1%, not detected previously in their basal genotyping test. No resistance mutations were observed to Integrase Strand Transfer Inhibitors (INSTIs). We identified a possible cause of VF in ART-naïve subjects with low-frequency mutations detected. To our knowledge, this is the first evaluation of pre-existing drug resistance for HIV-1 minority variants carried out on ART-naïve people living with HIV/AIDS (PLWHA) by analyzing the HIV-1 pol gene using NGS in the country. MDPI 2023-08-01 /pmc/articles/PMC10454674/ /pubmed/37623048 http://dx.doi.org/10.3390/idr15040044 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Moreno, Ambar
González, Claudia
Góndola, Jessica
Chavarría, Oris
Ortiz, Alma
Castillo, Jorge
Castillo Mewa, Juan
Pascale, Juan Miguel
Martínez, Alexander Augusto
HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title_full HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title_fullStr HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title_full_unstemmed HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title_short HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
title_sort hiv-1 low-frequency variants identified in antiretroviral-naïve subjects with virologic failure after 12 months of follow-up in panama
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454674/
https://www.ncbi.nlm.nih.gov/pubmed/37623048
http://dx.doi.org/10.3390/idr15040044
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