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Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hol...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454675/ https://www.ncbi.nlm.nih.gov/pubmed/37629020 http://dx.doi.org/10.3390/ijms241612841 |
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author | Kawai-Harada, Yuki El Itawi, Hanine Komuro, Hiroaki Harada, Masako |
author_facet | Kawai-Harada, Yuki El Itawi, Hanine Komuro, Hiroaki Harada, Masako |
author_sort | Kawai-Harada, Yuki |
collection | PubMed |
description | Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings. Previously, several groups demonstrated the problems of commonly used storage methods that distort EV integrity. This work aims to evaluate the process to optimize the storage conditions of EVs and then characterize them according to the experimental conditions and the models used previously. Our study reports a highly efficient EV storage condition, focusing on EV capacity to protect their molecular cargo from biological, chemical, and mechanical damage. Compared with commonly used EV storage conditions, our EV storage buffer leads to less size and particle number variation at both 4 °C and −80 °C, enhancing the ability to protect EVs while maintaining targeting functionality. |
format | Online Article Text |
id | pubmed-10454675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104546752023-08-26 Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles Kawai-Harada, Yuki El Itawi, Hanine Komuro, Hiroaki Harada, Masako Int J Mol Sci Article Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings. Previously, several groups demonstrated the problems of commonly used storage methods that distort EV integrity. This work aims to evaluate the process to optimize the storage conditions of EVs and then characterize them according to the experimental conditions and the models used previously. Our study reports a highly efficient EV storage condition, focusing on EV capacity to protect their molecular cargo from biological, chemical, and mechanical damage. Compared with commonly used EV storage conditions, our EV storage buffer leads to less size and particle number variation at both 4 °C and −80 °C, enhancing the ability to protect EVs while maintaining targeting functionality. MDPI 2023-08-16 /pmc/articles/PMC10454675/ /pubmed/37629020 http://dx.doi.org/10.3390/ijms241612841 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kawai-Harada, Yuki El Itawi, Hanine Komuro, Hiroaki Harada, Masako Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title | Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title_full | Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title_fullStr | Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title_full_unstemmed | Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title_short | Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles |
title_sort | evaluation of ev storage buffer for efficient preservation of engineered extracellular vesicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454675/ https://www.ncbi.nlm.nih.gov/pubmed/37629020 http://dx.doi.org/10.3390/ijms241612841 |
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