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Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles

Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hol...

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Detalles Bibliográficos
Autores principales: Kawai-Harada, Yuki, El Itawi, Hanine, Komuro, Hiroaki, Harada, Masako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454675/
https://www.ncbi.nlm.nih.gov/pubmed/37629020
http://dx.doi.org/10.3390/ijms241612841
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author Kawai-Harada, Yuki
El Itawi, Hanine
Komuro, Hiroaki
Harada, Masako
author_facet Kawai-Harada, Yuki
El Itawi, Hanine
Komuro, Hiroaki
Harada, Masako
author_sort Kawai-Harada, Yuki
collection PubMed
description Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings. Previously, several groups demonstrated the problems of commonly used storage methods that distort EV integrity. This work aims to evaluate the process to optimize the storage conditions of EVs and then characterize them according to the experimental conditions and the models used previously. Our study reports a highly efficient EV storage condition, focusing on EV capacity to protect their molecular cargo from biological, chemical, and mechanical damage. Compared with commonly used EV storage conditions, our EV storage buffer leads to less size and particle number variation at both 4 °C and −80 °C, enhancing the ability to protect EVs while maintaining targeting functionality.
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spelling pubmed-104546752023-08-26 Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles Kawai-Harada, Yuki El Itawi, Hanine Komuro, Hiroaki Harada, Masako Int J Mol Sci Article Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings. Previously, several groups demonstrated the problems of commonly used storage methods that distort EV integrity. This work aims to evaluate the process to optimize the storage conditions of EVs and then characterize them according to the experimental conditions and the models used previously. Our study reports a highly efficient EV storage condition, focusing on EV capacity to protect their molecular cargo from biological, chemical, and mechanical damage. Compared with commonly used EV storage conditions, our EV storage buffer leads to less size and particle number variation at both 4 °C and −80 °C, enhancing the ability to protect EVs while maintaining targeting functionality. MDPI 2023-08-16 /pmc/articles/PMC10454675/ /pubmed/37629020 http://dx.doi.org/10.3390/ijms241612841 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kawai-Harada, Yuki
El Itawi, Hanine
Komuro, Hiroaki
Harada, Masako
Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title_full Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title_fullStr Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title_full_unstemmed Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title_short Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
title_sort evaluation of ev storage buffer for efficient preservation of engineered extracellular vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454675/
https://www.ncbi.nlm.nih.gov/pubmed/37629020
http://dx.doi.org/10.3390/ijms241612841
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