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Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat
The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, Clarias gariepinus. Juvenile fish were e...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454782/ https://www.ncbi.nlm.nih.gov/pubmed/34148463 http://dx.doi.org/10.1177/00368504211021751 |
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author | Ayanda, Opeyemi I Tolulope, Ajayi Oniye, Sonnie J |
author_facet | Ayanda, Opeyemi I Tolulope, Ajayi Oniye, Sonnie J |
author_sort | Ayanda, Opeyemi I |
collection | PubMed |
description | The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, Clarias gariepinus. Juvenile fish were exposed for 96 h to 0.36, 0.48, 0.60, 0.72 and 0.84 mg/L glyphosate; and 0.018, 0.037, 0.055, 0.110 and 0.221 mg/L paraquat. Following the exposure, the fish liver and blood were analysed for DNA damage and micronucleus respectively. DNA damage was analysed using comet assay while the micronucleus test was used for assessing nuclear abnormalities. Both herbicides induced DNA damage in fish, with paraquat exhibiting higher toxicity. The severity in liver DNA damage was observed to be dependent on concentration. The herbicides triggered formation of micronuclei, bean-shaped cells, lobed nuclei, and apoptosis in blood cells of fish. Both herbicides also increased the frequency of occurrence of these cell abnormalities in erythrocytes and showed mutagenic potential in fish. Glyphosate and Paraquat both have mutagenic potentials in fish and this is a reflection of the threat these contaminants pose to fish and other forms of aquatic life in our natural water bodies. Low concentrations of these herbicides should be encouraged when usage is inevitable |
format | Online Article Text |
id | pubmed-10454782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-104547822023-08-26 Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat Ayanda, Opeyemi I Tolulope, Ajayi Oniye, Sonnie J Sci Prog Article The aquatic ecosystem is under increasing pressure from environmental contaminants due to anthropogenic activities. This study investigated the potential of glyphosate and paraquat to induce DNA damage and other cell abnormalities in juvenile African Catfish, Clarias gariepinus. Juvenile fish were exposed for 96 h to 0.36, 0.48, 0.60, 0.72 and 0.84 mg/L glyphosate; and 0.018, 0.037, 0.055, 0.110 and 0.221 mg/L paraquat. Following the exposure, the fish liver and blood were analysed for DNA damage and micronucleus respectively. DNA damage was analysed using comet assay while the micronucleus test was used for assessing nuclear abnormalities. Both herbicides induced DNA damage in fish, with paraquat exhibiting higher toxicity. The severity in liver DNA damage was observed to be dependent on concentration. The herbicides triggered formation of micronuclei, bean-shaped cells, lobed nuclei, and apoptosis in blood cells of fish. Both herbicides also increased the frequency of occurrence of these cell abnormalities in erythrocytes and showed mutagenic potential in fish. Glyphosate and Paraquat both have mutagenic potentials in fish and this is a reflection of the threat these contaminants pose to fish and other forms of aquatic life in our natural water bodies. Low concentrations of these herbicides should be encouraged when usage is inevitable SAGE Publications 2021-06-19 /pmc/articles/PMC10454782/ /pubmed/34148463 http://dx.doi.org/10.1177/00368504211021751 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Article Ayanda, Opeyemi I Tolulope, Ajayi Oniye, Sonnie J Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title | Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title_full | Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title_fullStr | Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title_full_unstemmed | Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title_short | Mutagenicity and genotoxicity in juvenile African catfish, Clarias gariepinus exposed to formulations of glyphosate and paraquat |
title_sort | mutagenicity and genotoxicity in juvenile african catfish, clarias gariepinus exposed to formulations of glyphosate and paraquat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454782/ https://www.ncbi.nlm.nih.gov/pubmed/34148463 http://dx.doi.org/10.1177/00368504211021751 |
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