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Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components

Diatoms synthesize species-specific exoskeletons inside cells under the control of the cytoskeleton and microtubule center. Previous studies have been conducted with the visualization of the microtubule center; however, its composition has not been studied and reliably established. In the present st...

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Autores principales: Petrova, Darya P., Morozov, Alexey A., Potapova, Nadezhda A., Bedoshvili, Yekaterina D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454807/
https://www.ncbi.nlm.nih.gov/pubmed/37628962
http://dx.doi.org/10.3390/ijms241612781
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author Petrova, Darya P.
Morozov, Alexey A.
Potapova, Nadezhda A.
Bedoshvili, Yekaterina D.
author_facet Petrova, Darya P.
Morozov, Alexey A.
Potapova, Nadezhda A.
Bedoshvili, Yekaterina D.
author_sort Petrova, Darya P.
collection PubMed
description Diatoms synthesize species-specific exoskeletons inside cells under the control of the cytoskeleton and microtubule center. Previous studies have been conducted with the visualization of the microtubule center; however, its composition has not been studied and reliably established. In the present study, several components of MTOC in diatoms, GCP (gamma complex proteins), Aurora A, and centrins have been identified. Analysis of the predicted amino acid sequences of these proteins revealed structural features typical for diatoms. We analyzed the conserved amino acids and the motives necessary for the functioning of proteins. Phylogenetic analysis of GCP showed that all major groups of diatoms are distributed over phylogenetic trees according to their systematic position. This work is a theoretical study; however, it allows drawing some conclusions about the functioning of the studied components and possible ways to regulate them.
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spelling pubmed-104548072023-08-26 Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components Petrova, Darya P. Morozov, Alexey A. Potapova, Nadezhda A. Bedoshvili, Yekaterina D. Int J Mol Sci Article Diatoms synthesize species-specific exoskeletons inside cells under the control of the cytoskeleton and microtubule center. Previous studies have been conducted with the visualization of the microtubule center; however, its composition has not been studied and reliably established. In the present study, several components of MTOC in diatoms, GCP (gamma complex proteins), Aurora A, and centrins have been identified. Analysis of the predicted amino acid sequences of these proteins revealed structural features typical for diatoms. We analyzed the conserved amino acids and the motives necessary for the functioning of proteins. Phylogenetic analysis of GCP showed that all major groups of diatoms are distributed over phylogenetic trees according to their systematic position. This work is a theoretical study; however, it allows drawing some conclusions about the functioning of the studied components and possible ways to regulate them. MDPI 2023-08-14 /pmc/articles/PMC10454807/ /pubmed/37628962 http://dx.doi.org/10.3390/ijms241612781 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrova, Darya P.
Morozov, Alexey A.
Potapova, Nadezhda A.
Bedoshvili, Yekaterina D.
Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title_full Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title_fullStr Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title_full_unstemmed Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title_short Analysis of Predicted Amino Acid Sequences of Diatom Microtubule Center Components
title_sort analysis of predicted amino acid sequences of diatom microtubule center components
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454807/
https://www.ncbi.nlm.nih.gov/pubmed/37628962
http://dx.doi.org/10.3390/ijms241612781
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