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In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation
The clinical use of anthracycline Doxorubicin as an antineoplastic drug in cancer therapy is limited by cardiotoxic effects that can lead to congestive heart failure. Recent studies have shown several promising activities of different species of the genus Ferula belonging to the Apiaceae Family. Fer...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454821/ https://www.ncbi.nlm.nih.gov/pubmed/37628916 http://dx.doi.org/10.3390/ijms241612735 |
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author | Macrì, Roberta Bava, Irene Scarano, Federica Mollace, Rocco Musolino, Vincenzo Gliozzi, Micaela Greco, Marta Foti, Daniela Tucci, Luigi Maiuolo, Jessica Carresi, Cristina Tavernese, Annamaria Palma, Ernesto Muscoli, Carolina Mollace, Vincenzo |
author_facet | Macrì, Roberta Bava, Irene Scarano, Federica Mollace, Rocco Musolino, Vincenzo Gliozzi, Micaela Greco, Marta Foti, Daniela Tucci, Luigi Maiuolo, Jessica Carresi, Cristina Tavernese, Annamaria Palma, Ernesto Muscoli, Carolina Mollace, Vincenzo |
author_sort | Macrì, Roberta |
collection | PubMed |
description | The clinical use of anthracycline Doxorubicin as an antineoplastic drug in cancer therapy is limited by cardiotoxic effects that can lead to congestive heart failure. Recent studies have shown several promising activities of different species of the genus Ferula belonging to the Apiaceae Family. Ferula communis is the main source of Ferutinin—a bioactive compound isolated from many species of Ferula—studied both in vitro and in vivo because of their different effects, such as estrogenic, antioxidant, anti-inflammatory, and also antiproliferative and cytotoxic activity, performed in a dose-dependent and cell-dependent way. However, the potential protective role of Ferutinin in myocardium impairment, caused by chemotherapeutic drugs, still represents an unexplored field. The aim of this study was to test the effects of Ferutinin rich-Ferula communis L. root extract (FcFE) at different concentrations on H9C2 cells. Moreover, we evaluated its antioxidant properties in cardiomyocytes in order to explore new potential therapeutic activities never examined before in other experimental works. FcFE, at a concentration of 0.25 µM, in the H9C2 line, significantly reduced the ROS production induced by H(2)O(2) (50 µM and 250 µM) and traced the cell mortality of the H9C2 co-treated with Ferutinin 0.25 µM and Doxorubicin (0.5 µM and 1 µM) to control levels. These results showed that FcFE could protect against Doxorubicin-induced cardiotoxicity. Further molecular characterization of this natural compound may open the way for testing FcFE at low concentrations in vivo and in clinical studies as an adjuvant in cancer therapy in association with anthracyclines to prevent side effects on heart cells. |
format | Online Article Text |
id | pubmed-10454821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104548212023-08-26 In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation Macrì, Roberta Bava, Irene Scarano, Federica Mollace, Rocco Musolino, Vincenzo Gliozzi, Micaela Greco, Marta Foti, Daniela Tucci, Luigi Maiuolo, Jessica Carresi, Cristina Tavernese, Annamaria Palma, Ernesto Muscoli, Carolina Mollace, Vincenzo Int J Mol Sci Communication The clinical use of anthracycline Doxorubicin as an antineoplastic drug in cancer therapy is limited by cardiotoxic effects that can lead to congestive heart failure. Recent studies have shown several promising activities of different species of the genus Ferula belonging to the Apiaceae Family. Ferula communis is the main source of Ferutinin—a bioactive compound isolated from many species of Ferula—studied both in vitro and in vivo because of their different effects, such as estrogenic, antioxidant, anti-inflammatory, and also antiproliferative and cytotoxic activity, performed in a dose-dependent and cell-dependent way. However, the potential protective role of Ferutinin in myocardium impairment, caused by chemotherapeutic drugs, still represents an unexplored field. The aim of this study was to test the effects of Ferutinin rich-Ferula communis L. root extract (FcFE) at different concentrations on H9C2 cells. Moreover, we evaluated its antioxidant properties in cardiomyocytes in order to explore new potential therapeutic activities never examined before in other experimental works. FcFE, at a concentration of 0.25 µM, in the H9C2 line, significantly reduced the ROS production induced by H(2)O(2) (50 µM and 250 µM) and traced the cell mortality of the H9C2 co-treated with Ferutinin 0.25 µM and Doxorubicin (0.5 µM and 1 µM) to control levels. These results showed that FcFE could protect against Doxorubicin-induced cardiotoxicity. Further molecular characterization of this natural compound may open the way for testing FcFE at low concentrations in vivo and in clinical studies as an adjuvant in cancer therapy in association with anthracyclines to prevent side effects on heart cells. MDPI 2023-08-13 /pmc/articles/PMC10454821/ /pubmed/37628916 http://dx.doi.org/10.3390/ijms241612735 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Macrì, Roberta Bava, Irene Scarano, Federica Mollace, Rocco Musolino, Vincenzo Gliozzi, Micaela Greco, Marta Foti, Daniela Tucci, Luigi Maiuolo, Jessica Carresi, Cristina Tavernese, Annamaria Palma, Ernesto Muscoli, Carolina Mollace, Vincenzo In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title | In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title_full | In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title_fullStr | In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title_full_unstemmed | In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title_short | In Vitro Evaluation of Ferutinin Rich-Ferula communis L., ssp. glauca, Root Extract on Doxorubicin-Induced Cardiotoxicity: Antioxidant Properties and Cell Cycle Modulation |
title_sort | in vitro evaluation of ferutinin rich-ferula communis l., ssp. glauca, root extract on doxorubicin-induced cardiotoxicity: antioxidant properties and cell cycle modulation |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454821/ https://www.ncbi.nlm.nih.gov/pubmed/37628916 http://dx.doi.org/10.3390/ijms241612735 |
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