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Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury
(1) Background: Despite the evidence that ferroptosis is involved in myocardial ischemia-reperfusion (MIR), the critical regulator of ferroptosis in MIR remains unclear. (2) Methods: We included three GEO datasets and a set of ferroptosis-related genes with 259 genes. Following the identification of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10455596/ https://www.ncbi.nlm.nih.gov/pubmed/37623357 http://dx.doi.org/10.3390/jcdd10080344 |
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author | Wei, Xiuxian Li, Yi Luo, Pengcheng Dai, Yue Jiang, Tao Xu, Mulin Hao, Yi Zhang, Cuntai Liu, Yu |
author_facet | Wei, Xiuxian Li, Yi Luo, Pengcheng Dai, Yue Jiang, Tao Xu, Mulin Hao, Yi Zhang, Cuntai Liu, Yu |
author_sort | Wei, Xiuxian |
collection | PubMed |
description | (1) Background: Despite the evidence that ferroptosis is involved in myocardial ischemia-reperfusion (MIR), the critical regulator of ferroptosis in MIR remains unclear. (2) Methods: We included three GEO datasets and a set of ferroptosis-related genes with 259 genes. Following the identification of the differentially expressed ferroptosis-related genes (DEFRGs) and hub genes, we performed the functional annotation, protein–protein interaction network, and immune infiltration analysis. The GSE168610 dataset, a cell model, and an animal model were then used to verify key genes. (3) Results: We identified 17 DEFRGs and 9 hub genes in the MIR samples compared to the control. Heme oxygenase 1 (Hmox1), activating transcription factor 3 (Atf3), epidermal growth factor receptor (Egfr), and X-box binding protein 1 (Xbp1) were significantly upregulated in response to ischemic and hypoxic stimuli. In contrast, glutathione peroxidase 4 (Gpx4) and vascular endothelial growth factor A (Vegfa) were consistently decreased in either the oxygen and glucose deprivation/reoxygenation cell or the MIR mouse model. (4) Conclusions: This study emphasized the relevance of ferroptosis in MIR. It has been successfully demonstrated that nine ferroptosis-related genes (Hmox1, Atf3, Egfr, Gpx4, Cd44, Vegfa, asparagine synthetase (Asns), Xbp1, and bromodomain containing 4 (Brd4)) are involved in the process. Additional studies are needed to explore potential therapeutic targets for MIR. |
format | Online Article Text |
id | pubmed-10455596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104555962023-08-26 Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury Wei, Xiuxian Li, Yi Luo, Pengcheng Dai, Yue Jiang, Tao Xu, Mulin Hao, Yi Zhang, Cuntai Liu, Yu J Cardiovasc Dev Dis Article (1) Background: Despite the evidence that ferroptosis is involved in myocardial ischemia-reperfusion (MIR), the critical regulator of ferroptosis in MIR remains unclear. (2) Methods: We included three GEO datasets and a set of ferroptosis-related genes with 259 genes. Following the identification of the differentially expressed ferroptosis-related genes (DEFRGs) and hub genes, we performed the functional annotation, protein–protein interaction network, and immune infiltration analysis. The GSE168610 dataset, a cell model, and an animal model were then used to verify key genes. (3) Results: We identified 17 DEFRGs and 9 hub genes in the MIR samples compared to the control. Heme oxygenase 1 (Hmox1), activating transcription factor 3 (Atf3), epidermal growth factor receptor (Egfr), and X-box binding protein 1 (Xbp1) were significantly upregulated in response to ischemic and hypoxic stimuli. In contrast, glutathione peroxidase 4 (Gpx4) and vascular endothelial growth factor A (Vegfa) were consistently decreased in either the oxygen and glucose deprivation/reoxygenation cell or the MIR mouse model. (4) Conclusions: This study emphasized the relevance of ferroptosis in MIR. It has been successfully demonstrated that nine ferroptosis-related genes (Hmox1, Atf3, Egfr, Gpx4, Cd44, Vegfa, asparagine synthetase (Asns), Xbp1, and bromodomain containing 4 (Brd4)) are involved in the process. Additional studies are needed to explore potential therapeutic targets for MIR. MDPI 2023-08-12 /pmc/articles/PMC10455596/ /pubmed/37623357 http://dx.doi.org/10.3390/jcdd10080344 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wei, Xiuxian Li, Yi Luo, Pengcheng Dai, Yue Jiang, Tao Xu, Mulin Hao, Yi Zhang, Cuntai Liu, Yu Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title | Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title_full | Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title_fullStr | Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title_full_unstemmed | Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title_short | Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury |
title_sort | development and validation of robust ferroptosis-related genes in myocardial ischemia-reperfusion injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10455596/ https://www.ncbi.nlm.nih.gov/pubmed/37623357 http://dx.doi.org/10.3390/jcdd10080344 |
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