Cargando…

Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers

One of the blends that is usable for 3D printing while not being toxic to cell cultures is the lactic acid (PLA)/polyhydroxybutyrate (PHB)/thermoplastic starch (TPS) blend. The addition of plasticizers can change the rate of biodegradation and the biological behavior of the material. In order to eva...

Descripción completa

Detalles Bibliográficos
Autores principales: Trebuňová, Marianna, Petroušková, Patrícia, Balogová, Alena Findrik, Ižaríková, Gabriela, Horňak, Peter, Bačenková, Darina, Demeterová, Jana, Živčák, Jozef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10455870/
https://www.ncbi.nlm.nih.gov/pubmed/37623657
http://dx.doi.org/10.3390/jfb14080412
_version_ 1785096556513329152
author Trebuňová, Marianna
Petroušková, Patrícia
Balogová, Alena Findrik
Ižaríková, Gabriela
Horňak, Peter
Bačenková, Darina
Demeterová, Jana
Živčák, Jozef
author_facet Trebuňová, Marianna
Petroušková, Patrícia
Balogová, Alena Findrik
Ižaríková, Gabriela
Horňak, Peter
Bačenková, Darina
Demeterová, Jana
Živčák, Jozef
author_sort Trebuňová, Marianna
collection PubMed
description One of the blends that is usable for 3D printing while not being toxic to cell cultures is the lactic acid (PLA)/polyhydroxybutyrate (PHB)/thermoplastic starch (TPS) blend. The addition of plasticizers can change the rate of biodegradation and the biological behavior of the material. In order to evaluate the potential of the PLA/PHB/TPS material in combination with additives (plasticizers: acetyl tributyl citrate (ATBC) and oligomeric lactic acid (OLA)), for use in the field of biomedical tissue engineering, we performed a comprehensive in vitro characterization of selected mixture materials. Three types of materials were tested: I: PLA/PHB/TPS + 25% OLA, II: PLA/PHB/TPS + 30% ATBC, and III: PLA/PHB/TPS + 30% OLA. The assessment of the biocompatibility of the materials included cytotoxicity tests, such as monitoring the viability, proliferation and morphology of cells and their deposition on the surface of the materials. The cell line 7F2 osteoblasts (Mus musculus) was used in the experiments. Based on the test results, the significant influence of plasticizers on the material was confirmed, with their specific proportions in the mixtures. PLA/PHB/TPS + 25% OLA was evaluated as the optimal material for biocompatibility with 7F2 osteoblasts. The tested biomaterials have the potential for further investigation with a possible change in the proportion of plasticizers, which can have a fundamental impact on their biological properties.
format Online
Article
Text
id pubmed-10455870
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104558702023-08-26 Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers Trebuňová, Marianna Petroušková, Patrícia Balogová, Alena Findrik Ižaríková, Gabriela Horňak, Peter Bačenková, Darina Demeterová, Jana Živčák, Jozef J Funct Biomater Article One of the blends that is usable for 3D printing while not being toxic to cell cultures is the lactic acid (PLA)/polyhydroxybutyrate (PHB)/thermoplastic starch (TPS) blend. The addition of plasticizers can change the rate of biodegradation and the biological behavior of the material. In order to evaluate the potential of the PLA/PHB/TPS material in combination with additives (plasticizers: acetyl tributyl citrate (ATBC) and oligomeric lactic acid (OLA)), for use in the field of biomedical tissue engineering, we performed a comprehensive in vitro characterization of selected mixture materials. Three types of materials were tested: I: PLA/PHB/TPS + 25% OLA, II: PLA/PHB/TPS + 30% ATBC, and III: PLA/PHB/TPS + 30% OLA. The assessment of the biocompatibility of the materials included cytotoxicity tests, such as monitoring the viability, proliferation and morphology of cells and their deposition on the surface of the materials. The cell line 7F2 osteoblasts (Mus musculus) was used in the experiments. Based on the test results, the significant influence of plasticizers on the material was confirmed, with their specific proportions in the mixtures. PLA/PHB/TPS + 25% OLA was evaluated as the optimal material for biocompatibility with 7F2 osteoblasts. The tested biomaterials have the potential for further investigation with a possible change in the proportion of plasticizers, which can have a fundamental impact on their biological properties. MDPI 2023-08-04 /pmc/articles/PMC10455870/ /pubmed/37623657 http://dx.doi.org/10.3390/jfb14080412 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trebuňová, Marianna
Petroušková, Patrícia
Balogová, Alena Findrik
Ižaríková, Gabriela
Horňak, Peter
Bačenková, Darina
Demeterová, Jana
Živčák, Jozef
Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title_full Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title_fullStr Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title_full_unstemmed Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title_short Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers
title_sort evaluation of biocompatibility of pla/phb/tps polymer scaffolds with different additives of atbc and ola plasticizers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10455870/
https://www.ncbi.nlm.nih.gov/pubmed/37623657
http://dx.doi.org/10.3390/jfb14080412
work_keys_str_mv AT trebunovamarianna evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT petrouskovapatricia evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT balogovaalenafindrik evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT izarikovagabriela evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT hornakpeter evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT bacenkovadarina evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT demeterovajana evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers
AT zivcakjozef evaluationofbiocompatibilityofplaphbtpspolymerscaffoldswithdifferentadditivesofatbcandolaplasticizers