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Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort

BACKGROUND: Antibiotic resistance represents a serious global health challenge, particularly with the emergence of strains resistant to last-resort antibiotics such as tigecycline, polymyxin B, and vancomycin. Urgent measures are required to alleviate this situation. To facilitate the judicious use...

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Autores principales: Xiao, Zhirou, Qu, Liping, Chen, Haijun, Liu, Wanting, Zhan, Yi, Ling, Jiahui, Shen, Hongwei, Yang, Ling, Chen, Dingqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456006/
https://www.ncbi.nlm.nih.gov/pubmed/37638072
http://dx.doi.org/10.2147/IDR.S404732
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author Xiao, Zhirou
Qu, Liping
Chen, Haijun
Liu, Wanting
Zhan, Yi
Ling, Jiahui
Shen, Hongwei
Yang, Ling
Chen, Dingqiang
author_facet Xiao, Zhirou
Qu, Liping
Chen, Haijun
Liu, Wanting
Zhan, Yi
Ling, Jiahui
Shen, Hongwei
Yang, Ling
Chen, Dingqiang
author_sort Xiao, Zhirou
collection PubMed
description BACKGROUND: Antibiotic resistance represents a serious global health challenge, particularly with the emergence of strains resistant to last-resort antibiotics such as tigecycline, polymyxin B, and vancomycin. Urgent measures are required to alleviate this situation. To facilitate the judicious use of antibiotics, rapid and precise antimicrobial susceptibility testing (AST) is essential. Heavy water (deuterium oxide, D(2)O)-labeled Raman spectroscopy has emerged as a promising time-saving tool for microbiological testing. METHODS: Deuterium incorporation and experimental conditions were examined to develop and apply a Raman-based AST method to evaluate the efficacy of last-resort antibiotics, including tigecycline, polymyxin B, and vancomycin, against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterococcus faecium. Essential agreement and categorical agreement were used to assess the metabolism inactivation concentration based on Raman spectroscopy (R-MIC)–a new metric developed in this study—and minimum inhibitory concentration (MIC) determined via the traditional microdilution broth method. Spearman’s rank correlation coefficient was employed to measure the association between R-MIC and MIC values. RESULTS: The Raman-based AST method achieved a 100% categorical agreement (92/92) with the traditional microdilution broth method within five hours, while the traditional method required approximately 24 h. The R-MIC values shared 68.5% (63/92) consistency with the MIC values. In addition, the R-MIC and MIC values were highly correlated (Spearman’s r=0.96), resulting in an essential agreement of 100%. CONCLUSION: Our optimized experimental method and conditions indicate that Raman-based AST holds great promise as a solution to overcome the time-consuming challenges of traditional AST methods.
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spelling pubmed-104560062023-08-26 Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort Xiao, Zhirou Qu, Liping Chen, Haijun Liu, Wanting Zhan, Yi Ling, Jiahui Shen, Hongwei Yang, Ling Chen, Dingqiang Infect Drug Resist Original Research BACKGROUND: Antibiotic resistance represents a serious global health challenge, particularly with the emergence of strains resistant to last-resort antibiotics such as tigecycline, polymyxin B, and vancomycin. Urgent measures are required to alleviate this situation. To facilitate the judicious use of antibiotics, rapid and precise antimicrobial susceptibility testing (AST) is essential. Heavy water (deuterium oxide, D(2)O)-labeled Raman spectroscopy has emerged as a promising time-saving tool for microbiological testing. METHODS: Deuterium incorporation and experimental conditions were examined to develop and apply a Raman-based AST method to evaluate the efficacy of last-resort antibiotics, including tigecycline, polymyxin B, and vancomycin, against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterococcus faecium. Essential agreement and categorical agreement were used to assess the metabolism inactivation concentration based on Raman spectroscopy (R-MIC)–a new metric developed in this study—and minimum inhibitory concentration (MIC) determined via the traditional microdilution broth method. Spearman’s rank correlation coefficient was employed to measure the association between R-MIC and MIC values. RESULTS: The Raman-based AST method achieved a 100% categorical agreement (92/92) with the traditional microdilution broth method within five hours, while the traditional method required approximately 24 h. The R-MIC values shared 68.5% (63/92) consistency with the MIC values. In addition, the R-MIC and MIC values were highly correlated (Spearman’s r=0.96), resulting in an essential agreement of 100%. CONCLUSION: Our optimized experimental method and conditions indicate that Raman-based AST holds great promise as a solution to overcome the time-consuming challenges of traditional AST methods. Dove 2023-08-21 /pmc/articles/PMC10456006/ /pubmed/37638072 http://dx.doi.org/10.2147/IDR.S404732 Text en © 2023 Xiao et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Xiao, Zhirou
Qu, Liping
Chen, Haijun
Liu, Wanting
Zhan, Yi
Ling, Jiahui
Shen, Hongwei
Yang, Ling
Chen, Dingqiang
Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title_full Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title_fullStr Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title_full_unstemmed Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title_short Raman-Based Antimicrobial Susceptibility Testing on Antibiotics of Last Resort
title_sort raman-based antimicrobial susceptibility testing on antibiotics of last resort
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456006/
https://www.ncbi.nlm.nih.gov/pubmed/37638072
http://dx.doi.org/10.2147/IDR.S404732
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