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Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic

Soon after the beginning of the COVID-19 pandemic in early 2020, the Betacoronavirus SARS-CoV-2 infection of several mink farms breeding American minks (Neovison vison) for fur was detected in various European countries. The risk of a new reservoir being formed and of a reverse zoonosis from minks q...

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Autores principales: Wasniewski, Marine, Boué, Franck, Richomme, Céline, Simon-Lorière, Etienne, der Werf, Sylvie Van, Donati, Flora, Enouf, Vincent, Blanchard, Yannick, Beven, Véronique, Leperchois, Estelle, Leterrier, Bryce, Corbet, Sandrine, Le Gouil, Meriadeg, Monchatre-Leroy, Elodie, Picard-Meyer, Evelyne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456147/
https://www.ncbi.nlm.nih.gov/pubmed/37624818
http://dx.doi.org/10.1371/journal.pone.0290444
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author Wasniewski, Marine
Boué, Franck
Richomme, Céline
Simon-Lorière, Etienne
der Werf, Sylvie Van
Donati, Flora
Enouf, Vincent
Blanchard, Yannick
Beven, Véronique
Leperchois, Estelle
Leterrier, Bryce
Corbet, Sandrine
Le Gouil, Meriadeg
Monchatre-Leroy, Elodie
Picard-Meyer, Evelyne
author_facet Wasniewski, Marine
Boué, Franck
Richomme, Céline
Simon-Lorière, Etienne
der Werf, Sylvie Van
Donati, Flora
Enouf, Vincent
Blanchard, Yannick
Beven, Véronique
Leperchois, Estelle
Leterrier, Bryce
Corbet, Sandrine
Le Gouil, Meriadeg
Monchatre-Leroy, Elodie
Picard-Meyer, Evelyne
author_sort Wasniewski, Marine
collection PubMed
description Soon after the beginning of the COVID-19 pandemic in early 2020, the Betacoronavirus SARS-CoV-2 infection of several mink farms breeding American minks (Neovison vison) for fur was detected in various European countries. The risk of a new reservoir being formed and of a reverse zoonosis from minks quickly became a major concern. The aim of this study was to investigate the four French mink farms to see whether SARS-CoV-2 was circulating there in late 2020. The investigations took place during the slaughtering period, thus facilitating different types of sampling (swabs and blood). On one of the four mink farms, 96.6% of serum samples were positive when tested with a SARS-CoV-2 ELISA coated with purified N protein recombinant antigen, and 54 out of 162 (33%) pharyngo-tracheal swabs were positive by RT-qPCR. The genetic variability among 12 SARS-CoV-2 genomes sequenced from this farm indicated the co-circulation of several lineages at the time of sampling. All the SARS-CoV-2 genomes detected were nested within the 20A clade (Nextclade), together with SARS-CoV-2 genomes from humans sampled during the same period. The percentage of SARS-CoV-2 seropositivity by ELISA varied between 0.3 and 1.1% on the other three farms. Interestingly, among these three farms, 11 pharyngo-tracheal swabs and 3 fecal pools from two farms were positive by end-point RT-PCR for an Alphacoronavirus very similar to a mink coronavirus sequence observed on Danish farms in 2015. In addition, a mink Caliciviridae was identified on one of the two farms positive for Alphacoronavirus. The clinical impact of these inapparent viral infections is not known. The co-infection of SARS-CoV-2 with other viruses on mink farms could help explain the diversity of clinical symptoms noted on different infected farms in Europe. In addition, the co-circulation of an Alphacoronavirus and SARS-CoV-2 on a mink farm would potentially increase the risk of viral recombination between alpha and betacoronaviruses as already suggested in wild and domestic animals, as well as in humans.
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spelling pubmed-104561472023-08-26 Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic Wasniewski, Marine Boué, Franck Richomme, Céline Simon-Lorière, Etienne der Werf, Sylvie Van Donati, Flora Enouf, Vincent Blanchard, Yannick Beven, Véronique Leperchois, Estelle Leterrier, Bryce Corbet, Sandrine Le Gouil, Meriadeg Monchatre-Leroy, Elodie Picard-Meyer, Evelyne PLoS One Research Article Soon after the beginning of the COVID-19 pandemic in early 2020, the Betacoronavirus SARS-CoV-2 infection of several mink farms breeding American minks (Neovison vison) for fur was detected in various European countries. The risk of a new reservoir being formed and of a reverse zoonosis from minks quickly became a major concern. The aim of this study was to investigate the four French mink farms to see whether SARS-CoV-2 was circulating there in late 2020. The investigations took place during the slaughtering period, thus facilitating different types of sampling (swabs and blood). On one of the four mink farms, 96.6% of serum samples were positive when tested with a SARS-CoV-2 ELISA coated with purified N protein recombinant antigen, and 54 out of 162 (33%) pharyngo-tracheal swabs were positive by RT-qPCR. The genetic variability among 12 SARS-CoV-2 genomes sequenced from this farm indicated the co-circulation of several lineages at the time of sampling. All the SARS-CoV-2 genomes detected were nested within the 20A clade (Nextclade), together with SARS-CoV-2 genomes from humans sampled during the same period. The percentage of SARS-CoV-2 seropositivity by ELISA varied between 0.3 and 1.1% on the other three farms. Interestingly, among these three farms, 11 pharyngo-tracheal swabs and 3 fecal pools from two farms were positive by end-point RT-PCR for an Alphacoronavirus very similar to a mink coronavirus sequence observed on Danish farms in 2015. In addition, a mink Caliciviridae was identified on one of the two farms positive for Alphacoronavirus. The clinical impact of these inapparent viral infections is not known. The co-infection of SARS-CoV-2 with other viruses on mink farms could help explain the diversity of clinical symptoms noted on different infected farms in Europe. In addition, the co-circulation of an Alphacoronavirus and SARS-CoV-2 on a mink farm would potentially increase the risk of viral recombination between alpha and betacoronaviruses as already suggested in wild and domestic animals, as well as in humans. Public Library of Science 2023-08-25 /pmc/articles/PMC10456147/ /pubmed/37624818 http://dx.doi.org/10.1371/journal.pone.0290444 Text en © 2023 Wasniewski et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wasniewski, Marine
Boué, Franck
Richomme, Céline
Simon-Lorière, Etienne
der Werf, Sylvie Van
Donati, Flora
Enouf, Vincent
Blanchard, Yannick
Beven, Véronique
Leperchois, Estelle
Leterrier, Bryce
Corbet, Sandrine
Le Gouil, Meriadeg
Monchatre-Leroy, Elodie
Picard-Meyer, Evelyne
Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title_full Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title_fullStr Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title_full_unstemmed Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title_short Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
title_sort investigations into sars-cov-2 and other coronaviruses on mink farms in france late in the first year of the covid-19 pandemic
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456147/
https://www.ncbi.nlm.nih.gov/pubmed/37624818
http://dx.doi.org/10.1371/journal.pone.0290444
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