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Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spec...

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Autores principales: Boaks, Mawla, Roper, Connor, Viglione, Matthew, Hooper, Kent, Woolley, Adam T., Christensen, Kenneth A., Nordin, Gregory P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456398/
https://www.ncbi.nlm.nih.gov/pubmed/37630125
http://dx.doi.org/10.3390/mi14081589
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author Boaks, Mawla
Roper, Connor
Viglione, Matthew
Hooper, Kent
Woolley, Adam T.
Christensen, Kenneth A.
Nordin, Gregory P.
author_facet Boaks, Mawla
Roper, Connor
Viglione, Matthew
Hooper, Kent
Woolley, Adam T.
Christensen, Kenneth A.
Nordin, Gregory P.
author_sort Boaks, Mawla
collection PubMed
description We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spectrum. Complete overlap is essential to effectively limit the optical penetration depth, which is required to achieve high out-of-plane resolution. We demonstrate the high resolution in practice by 3D printing 15 [Formula: see text] m square pillars in a microfluidic chamber, where the pillars are separated by 7.7 [Formula: see text] m and are printed with 5 [Formula: see text] m layers. Furthermore, we show reliable membrane valves and pumps using the biocompatible resin. Valves are tested to 1,000,000 actuations with no observable degradation in performance. Finally, we create a concentration gradient generation (CG) component and utilize it in two device designs for cell chemotaxis studies. The first design relies on an external dual syringe pump to generate source and sink flows to supply the CG channel, while the second is a complete integrated device incorporating on-chip pumps, valves, and reservoirs. Both device types are seeded with adherent cells that are subjected to a chemoattractant CG, and both show clear evidence of chemotactic cellular migration. Moreover, the integrated device demonstrates cellular migration comparable to the external syringe pump device. This demonstration illustrates the effectiveness of our integrated chemotactic assay approach and high-resolution biocompatible resin 3D printing fabrication process. In addition, our 3D printing process has been tuned for rapid fabrication, as printing times for the two device designs are, respectively, 8 and 15 min.
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spelling pubmed-104563982023-08-26 Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration Boaks, Mawla Roper, Connor Viglione, Matthew Hooper, Kent Woolley, Adam T. Christensen, Kenneth A. Nordin, Gregory P. Micromachines (Basel) Article We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spectrum. Complete overlap is essential to effectively limit the optical penetration depth, which is required to achieve high out-of-plane resolution. We demonstrate the high resolution in practice by 3D printing 15 [Formula: see text] m square pillars in a microfluidic chamber, where the pillars are separated by 7.7 [Formula: see text] m and are printed with 5 [Formula: see text] m layers. Furthermore, we show reliable membrane valves and pumps using the biocompatible resin. Valves are tested to 1,000,000 actuations with no observable degradation in performance. Finally, we create a concentration gradient generation (CG) component and utilize it in two device designs for cell chemotaxis studies. The first design relies on an external dual syringe pump to generate source and sink flows to supply the CG channel, while the second is a complete integrated device incorporating on-chip pumps, valves, and reservoirs. Both device types are seeded with adherent cells that are subjected to a chemoattractant CG, and both show clear evidence of chemotactic cellular migration. Moreover, the integrated device demonstrates cellular migration comparable to the external syringe pump device. This demonstration illustrates the effectiveness of our integrated chemotactic assay approach and high-resolution biocompatible resin 3D printing fabrication process. In addition, our 3D printing process has been tuned for rapid fabrication, as printing times for the two device designs are, respectively, 8 and 15 min. MDPI 2023-08-12 /pmc/articles/PMC10456398/ /pubmed/37630125 http://dx.doi.org/10.3390/mi14081589 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boaks, Mawla
Roper, Connor
Viglione, Matthew
Hooper, Kent
Woolley, Adam T.
Christensen, Kenneth A.
Nordin, Gregory P.
Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title_full Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title_fullStr Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title_full_unstemmed Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title_short Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration
title_sort biocompatible high-resolution 3d-printed microfluidic devices: integrated cell chemotaxis demonstration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456398/
https://www.ncbi.nlm.nih.gov/pubmed/37630125
http://dx.doi.org/10.3390/mi14081589
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