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Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material

To gain confidence in results of omic-data acquisitions, methods must be benchmarked using validated quality control materials. We report data combining both untargeted and targeted metabolomics assays for the analysis of four new human fecal reference materials developed by the U.S. National Instit...

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Autores principales: Cumeras, Raquel, Shen, Tong, Valdiviez, Luis, Tippins, Zakery, Haffner, Bennett D., Fiehn, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456543/
https://www.ncbi.nlm.nih.gov/pubmed/37623865
http://dx.doi.org/10.3390/metabo13080921
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author Cumeras, Raquel
Shen, Tong
Valdiviez, Luis
Tippins, Zakery
Haffner, Bennett D.
Fiehn, Oliver
author_facet Cumeras, Raquel
Shen, Tong
Valdiviez, Luis
Tippins, Zakery
Haffner, Bennett D.
Fiehn, Oliver
author_sort Cumeras, Raquel
collection PubMed
description To gain confidence in results of omic-data acquisitions, methods must be benchmarked using validated quality control materials. We report data combining both untargeted and targeted metabolomics assays for the analysis of four new human fecal reference materials developed by the U.S. National Institute of Standards and Technologies (NIST) for metagenomics and metabolomics measurements. These reference grade test materials (RGTM) were established by NIST based on two different diets and two different samples treatments, as follows: firstly, homogenized fecal matter from subjects eating vegan diets, stored and submitted in either lyophilized (RGTM 10162) or aqueous form (RGTM 10171); secondly, homogenized fecal matter from subjects eating omnivore diets, stored and submitted in either lyophilized (RGTM 10172) or aqueous form (RGTM 10173). We used four untargeted metabolomics assays (lipidomics, primary metabolites, biogenic amines and polyphenols) and one targeted assay on bile acids. A total of 3563 compounds were annotated by mass spectrometry, including 353 compounds that were annotated in more than one assay. Almost half of all compounds were annotated using hydrophilic interaction chromatography/accurate mass spectrometry, followed by the lipidomics and the polyphenol assays. In total, 910 metabolites were found in at least 4-fold different levels in fecal matter from vegans versus omnivores, specifically for peptides, amino acids and lipids. In comparison, only 251 compounds showed 4-fold differences between lyophilized and aqueous fecal samples, including DG O-34:0 and methionine sulfoxide. A range of diet-specific metabolites were identified to be significantly different between vegans and omnivores, exemplified by citrinin and C17:0-acylcarnitine for omnivores, and curcumin and lenticin for vegans. Bioactive molecules like acyl alpha-hydroxy-fatty acids (AAHFA) were differentially regulated in vegan versus omnivore fecal materials, highlighting the importance of diet-specific reference materials for dietary biomarker studies.
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spelling pubmed-104565432023-08-26 Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material Cumeras, Raquel Shen, Tong Valdiviez, Luis Tippins, Zakery Haffner, Bennett D. Fiehn, Oliver Metabolites Article To gain confidence in results of omic-data acquisitions, methods must be benchmarked using validated quality control materials. We report data combining both untargeted and targeted metabolomics assays for the analysis of four new human fecal reference materials developed by the U.S. National Institute of Standards and Technologies (NIST) for metagenomics and metabolomics measurements. These reference grade test materials (RGTM) were established by NIST based on two different diets and two different samples treatments, as follows: firstly, homogenized fecal matter from subjects eating vegan diets, stored and submitted in either lyophilized (RGTM 10162) or aqueous form (RGTM 10171); secondly, homogenized fecal matter from subjects eating omnivore diets, stored and submitted in either lyophilized (RGTM 10172) or aqueous form (RGTM 10173). We used four untargeted metabolomics assays (lipidomics, primary metabolites, biogenic amines and polyphenols) and one targeted assay on bile acids. A total of 3563 compounds were annotated by mass spectrometry, including 353 compounds that were annotated in more than one assay. Almost half of all compounds were annotated using hydrophilic interaction chromatography/accurate mass spectrometry, followed by the lipidomics and the polyphenol assays. In total, 910 metabolites were found in at least 4-fold different levels in fecal matter from vegans versus omnivores, specifically for peptides, amino acids and lipids. In comparison, only 251 compounds showed 4-fold differences between lyophilized and aqueous fecal samples, including DG O-34:0 and methionine sulfoxide. A range of diet-specific metabolites were identified to be significantly different between vegans and omnivores, exemplified by citrinin and C17:0-acylcarnitine for omnivores, and curcumin and lenticin for vegans. Bioactive molecules like acyl alpha-hydroxy-fatty acids (AAHFA) were differentially regulated in vegan versus omnivore fecal materials, highlighting the importance of diet-specific reference materials for dietary biomarker studies. MDPI 2023-08-07 /pmc/articles/PMC10456543/ /pubmed/37623865 http://dx.doi.org/10.3390/metabo13080921 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cumeras, Raquel
Shen, Tong
Valdiviez, Luis
Tippins, Zakery
Haffner, Bennett D.
Fiehn, Oliver
Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title_full Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title_fullStr Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title_full_unstemmed Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title_short Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material
title_sort differences in the stool metabolome between vegans and omnivores: analyzing the nist stool reference material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456543/
https://www.ncbi.nlm.nih.gov/pubmed/37623865
http://dx.doi.org/10.3390/metabo13080921
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