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Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization
Choroidal neovascularization (CNV) is a severe complication observed in individuals with pathological myopia (PM). Our hypothesis is that specific metabolic alterations occur during the development of CNV in patients with PM. To investigate this, an untargeted metabolomics analysis was conducted usi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456621/ https://www.ncbi.nlm.nih.gov/pubmed/37623844 http://dx.doi.org/10.3390/metabo13080900 |
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author | Wei, Qiaoling Yu, Zhiqiang Zhou, Xianjin Gong, Ruowen Jiang, Rui Xu, Gezhi Liu, Wei |
author_facet | Wei, Qiaoling Yu, Zhiqiang Zhou, Xianjin Gong, Ruowen Jiang, Rui Xu, Gezhi Liu, Wei |
author_sort | Wei, Qiaoling |
collection | PubMed |
description | Choroidal neovascularization (CNV) is a severe complication observed in individuals with pathological myopia (PM). Our hypothesis is that specific metabolic alterations occur during the development of CNV in patients with PM. To investigate this, an untargeted metabolomics analysis was conducted using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) on aqueous humor (AH) samples obtained from meticulously matched PM patients, including those with CNV (n = 11) and without CNV (n = 11). The analysis aimed to identify differentially expressed metabolites between the two groups. Furthermore, the discriminative ability of each metabolite was evaluated using the area under the receiver operating characteristic curve (AUC). Enriched metabolic pathways were determined using the KEGG and MetaboAnalyst databases. Our results revealed the detection of 272 metabolites using GC–MS and 1457 metabolites using LC–MS in AH samples. Among them, 97 metabolites exhibited significant differential expression between the CNV and non-CNV groups. Noteworthy candidates, including D-citramalic acid, biphenyl, and isoleucylproline, demonstrated high AUC values ranging from 0.801 to 1, indicating their potential as disease biomarkers. Additionally, all three metabolites showed a strong association with retinal cystoid edema in CNV patients. Furthermore, the study identified 12 altered metabolic pathways, with five of them related to carbohydrate metabolism, suggesting their involvement in the occurrence of myopic CNV. These findings provide possible disease-specific biomarkers of CNV in PM and suggest the role of disturbed carbohydrate metabolism in its pathogenesis. Larger studies are needed to validate these findings. |
format | Online Article Text |
id | pubmed-10456621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104566212023-08-26 Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization Wei, Qiaoling Yu, Zhiqiang Zhou, Xianjin Gong, Ruowen Jiang, Rui Xu, Gezhi Liu, Wei Metabolites Article Choroidal neovascularization (CNV) is a severe complication observed in individuals with pathological myopia (PM). Our hypothesis is that specific metabolic alterations occur during the development of CNV in patients with PM. To investigate this, an untargeted metabolomics analysis was conducted using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) on aqueous humor (AH) samples obtained from meticulously matched PM patients, including those with CNV (n = 11) and without CNV (n = 11). The analysis aimed to identify differentially expressed metabolites between the two groups. Furthermore, the discriminative ability of each metabolite was evaluated using the area under the receiver operating characteristic curve (AUC). Enriched metabolic pathways were determined using the KEGG and MetaboAnalyst databases. Our results revealed the detection of 272 metabolites using GC–MS and 1457 metabolites using LC–MS in AH samples. Among them, 97 metabolites exhibited significant differential expression between the CNV and non-CNV groups. Noteworthy candidates, including D-citramalic acid, biphenyl, and isoleucylproline, demonstrated high AUC values ranging from 0.801 to 1, indicating their potential as disease biomarkers. Additionally, all three metabolites showed a strong association with retinal cystoid edema in CNV patients. Furthermore, the study identified 12 altered metabolic pathways, with five of them related to carbohydrate metabolism, suggesting their involvement in the occurrence of myopic CNV. These findings provide possible disease-specific biomarkers of CNV in PM and suggest the role of disturbed carbohydrate metabolism in its pathogenesis. Larger studies are needed to validate these findings. MDPI 2023-07-30 /pmc/articles/PMC10456621/ /pubmed/37623844 http://dx.doi.org/10.3390/metabo13080900 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wei, Qiaoling Yu, Zhiqiang Zhou, Xianjin Gong, Ruowen Jiang, Rui Xu, Gezhi Liu, Wei Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title | Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title_full | Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title_fullStr | Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title_full_unstemmed | Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title_short | Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization |
title_sort | metabolomic profiling of aqueous humor from pathological myopia patients with choroidal neovascularization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456621/ https://www.ncbi.nlm.nih.gov/pubmed/37623844 http://dx.doi.org/10.3390/metabo13080900 |
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