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Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting

Dielectrophoresis technology is applied to microfluidic chips to achieve microscopic control of cells. Currently, microfluidic chips based on dielectrophoresis have certain limitations in terms of cell sorting species, in order to explore a microfluidic chip with excellent performance and high versa...

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Detalles Bibliográficos
Autores principales: Nan, Xueli, Zhang, Jiale, Wang, Xin, Kang, Tongtong, Cao, Xinxin, Hao, Jinjin, Jia, Qikun, Qin, Bolin, Mei, Shixuan, Xu, Zhikuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456708/
https://www.ncbi.nlm.nih.gov/pubmed/37630097
http://dx.doi.org/10.3390/mi14081561
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author Nan, Xueli
Zhang, Jiale
Wang, Xin
Kang, Tongtong
Cao, Xinxin
Hao, Jinjin
Jia, Qikun
Qin, Bolin
Mei, Shixuan
Xu, Zhikuan
author_facet Nan, Xueli
Zhang, Jiale
Wang, Xin
Kang, Tongtong
Cao, Xinxin
Hao, Jinjin
Jia, Qikun
Qin, Bolin
Mei, Shixuan
Xu, Zhikuan
author_sort Nan, Xueli
collection PubMed
description Dielectrophoresis technology is applied to microfluidic chips to achieve microscopic control of cells. Currently, microfluidic chips based on dielectrophoresis have certain limitations in terms of cell sorting species, in order to explore a microfluidic chip with excellent performance and high versatility. In this paper, we designed a microfluidic chip that can be used for continuous cell sorting, with the structural design of a curved channel and curved double side electrodes. CM factors were calculated for eight human healthy blood cells and cancerous cells using the software MyDEP, the simulation of various blood cells sorting and the simulation of the joule heat effect of the microfluidic chip were completed using the software COMSOL Multiphysics. The effect of voltage and inlet flow velocity on the simulation results was discussed using the control variables method. We found feasible parameters from simulation results under different voltages and inlet flow velocities, and the feasibility of the design was verified from multiple perspectives by measuring cell movement trajectories, cell recovery rate and separation purity. This paper provides a universal method for cell, particle and even protein sorting.
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spelling pubmed-104567082023-08-26 Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting Nan, Xueli Zhang, Jiale Wang, Xin Kang, Tongtong Cao, Xinxin Hao, Jinjin Jia, Qikun Qin, Bolin Mei, Shixuan Xu, Zhikuan Micromachines (Basel) Article Dielectrophoresis technology is applied to microfluidic chips to achieve microscopic control of cells. Currently, microfluidic chips based on dielectrophoresis have certain limitations in terms of cell sorting species, in order to explore a microfluidic chip with excellent performance and high versatility. In this paper, we designed a microfluidic chip that can be used for continuous cell sorting, with the structural design of a curved channel and curved double side electrodes. CM factors were calculated for eight human healthy blood cells and cancerous cells using the software MyDEP, the simulation of various blood cells sorting and the simulation of the joule heat effect of the microfluidic chip were completed using the software COMSOL Multiphysics. The effect of voltage and inlet flow velocity on the simulation results was discussed using the control variables method. We found feasible parameters from simulation results under different voltages and inlet flow velocities, and the feasibility of the design was verified from multiple perspectives by measuring cell movement trajectories, cell recovery rate and separation purity. This paper provides a universal method for cell, particle and even protein sorting. MDPI 2023-08-05 /pmc/articles/PMC10456708/ /pubmed/37630097 http://dx.doi.org/10.3390/mi14081561 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nan, Xueli
Zhang, Jiale
Wang, Xin
Kang, Tongtong
Cao, Xinxin
Hao, Jinjin
Jia, Qikun
Qin, Bolin
Mei, Shixuan
Xu, Zhikuan
Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title_full Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title_fullStr Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title_full_unstemmed Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title_short Design of a Low-Frequency Dielectrophoresis-Based Arc Microfluidic Chip for Multigroup Cell Sorting
title_sort design of a low-frequency dielectrophoresis-based arc microfluidic chip for multigroup cell sorting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456708/
https://www.ncbi.nlm.nih.gov/pubmed/37630097
http://dx.doi.org/10.3390/mi14081561
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