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Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero

The aim of this work was to achieve a better understanding of the bacterial pathogens associated with stillbirths that would serve to inform clinical interventions directed at reducing this adverse pregnancy outcome. A prospective observational study was conducted with the participation of 22 women...

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Autores principales: Holliday, Mira, Uddipto, Kumar, Castillo, Gerardo, Vera, Luz Estela, Quinlivan, Julie A., Mendz, George L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456767/
https://www.ncbi.nlm.nih.gov/pubmed/37630436
http://dx.doi.org/10.3390/microorganisms11081877
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author Holliday, Mira
Uddipto, Kumar
Castillo, Gerardo
Vera, Luz Estela
Quinlivan, Julie A.
Mendz, George L.
author_facet Holliday, Mira
Uddipto, Kumar
Castillo, Gerardo
Vera, Luz Estela
Quinlivan, Julie A.
Mendz, George L.
author_sort Holliday, Mira
collection PubMed
description The aim of this work was to achieve a better understanding of the bacterial pathogens associated with stillbirths that would serve to inform clinical interventions directed at reducing this adverse pregnancy outcome. A prospective observational study was conducted with the participation of 22 women from northern Peru, of whom 11 experienced fetal death in utero and 11 delivered preterm births. Swabs were taken from the vagina, placenta, amniotic fluid and axilla of the infant at birth by Caesarean section. The bacterial populations in the vagina and the amniotic space of each participant were determined by employing the amplicon sequencing of the V4 region of the 16S rRNA genes. The sequence data were analysed using bioinformatics tools. The work showed differences in the composition of the genital microbiomes of women who experienced preterm birth or fetal death in utero. There were no differences in the alpha diversity between the genital microbiotas of both groups of women, but there were more different taxa in the vagina and amniotic space of the preterm participants. Lactobacillus spp. was less abundant in the stillbirth cases. E. coli/Shigella, Staphylococcus, Gardnerella, Listeria and Bacteroides taxa were associated with the stillbirths. In each woman, there was a minimal concordance between the bacterial populations in the vagina and amniotic space.
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spelling pubmed-104567672023-08-26 Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero Holliday, Mira Uddipto, Kumar Castillo, Gerardo Vera, Luz Estela Quinlivan, Julie A. Mendz, George L. Microorganisms Article The aim of this work was to achieve a better understanding of the bacterial pathogens associated with stillbirths that would serve to inform clinical interventions directed at reducing this adverse pregnancy outcome. A prospective observational study was conducted with the participation of 22 women from northern Peru, of whom 11 experienced fetal death in utero and 11 delivered preterm births. Swabs were taken from the vagina, placenta, amniotic fluid and axilla of the infant at birth by Caesarean section. The bacterial populations in the vagina and the amniotic space of each participant were determined by employing the amplicon sequencing of the V4 region of the 16S rRNA genes. The sequence data were analysed using bioinformatics tools. The work showed differences in the composition of the genital microbiomes of women who experienced preterm birth or fetal death in utero. There were no differences in the alpha diversity between the genital microbiotas of both groups of women, but there were more different taxa in the vagina and amniotic space of the preterm participants. Lactobacillus spp. was less abundant in the stillbirth cases. E. coli/Shigella, Staphylococcus, Gardnerella, Listeria and Bacteroides taxa were associated with the stillbirths. In each woman, there was a minimal concordance between the bacterial populations in the vagina and amniotic space. MDPI 2023-07-25 /pmc/articles/PMC10456767/ /pubmed/37630436 http://dx.doi.org/10.3390/microorganisms11081877 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Holliday, Mira
Uddipto, Kumar
Castillo, Gerardo
Vera, Luz Estela
Quinlivan, Julie A.
Mendz, George L.
Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title_full Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title_fullStr Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title_full_unstemmed Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title_short Insights into the Genital Microbiota of Women Who Experienced Fetal Death in Utero
title_sort insights into the genital microbiota of women who experienced fetal death in utero
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456767/
https://www.ncbi.nlm.nih.gov/pubmed/37630436
http://dx.doi.org/10.3390/microorganisms11081877
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