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Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study

Antisepsis of the hands of medical personnel is one of the most important steps in the process of patient care, since direct contact can cause the cross-transfer of potentially pathogenic microorganisms at surgical sites. This study aimed to analyze the prevalence of microorganisms on the hands of 1...

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Autores principales: Serra Neto, Artur, Marques, Sirlei G., Bomfim, Maria Rosa Q., Monteiro, Silvio G., de Souza, Rosangela C., Nunes, Rodolfo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456775/
https://www.ncbi.nlm.nih.gov/pubmed/37630455
http://dx.doi.org/10.3390/microorganisms11081895
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author Serra Neto, Artur
Marques, Sirlei G.
Bomfim, Maria Rosa Q.
Monteiro, Silvio G.
de Souza, Rosangela C.
Nunes, Rodolfo A.
author_facet Serra Neto, Artur
Marques, Sirlei G.
Bomfim, Maria Rosa Q.
Monteiro, Silvio G.
de Souza, Rosangela C.
Nunes, Rodolfo A.
author_sort Serra Neto, Artur
collection PubMed
description Antisepsis of the hands of medical personnel is one of the most important steps in the process of patient care, since direct contact can cause the cross-transfer of potentially pathogenic microorganisms at surgical sites. This study aimed to analyze the prevalence of microorganisms on the hands of 131 surgeons in a university hospital before the surgical procedure. Swabs were collected from each clinician’s hands before and after handwashing. The samples were placed in a transport medium and immediately delivered to a private clinical analysis laboratory from São Luis-Maranhão. The microorganisms were identified by ionization source mass spectrometry and matrix-assisted laser desorption (MALDI-TOF), and antibiotic susceptibility tests (AST) were performed using the Vitek2 and Phoenix-BD automated system. The results showed a high frequency (100%) of microorganisms before handwashing, but after surgical antisepsis, the rate dropped significantly (p < 0.05) to 27.5%. The gram-positive species most detected were Staphylococcus spp. and Micrococcus luteus, representing 83.9%, followed by gram-negative species, Stenotrophomonas maltophilia, Acinetobacter baumanii, Pseudomonas aeruginosa, Pseudomonas gessardi, Pantoea septica, Serratia marcescens, and Burkholderia lata. The effectiveness of hand antisepsis was 72.5%, demonstrating that surgeons’ hands are an important source of microorganisms that can cause infections in hospitalized patients in different care settings.
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spelling pubmed-104567752023-08-26 Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study Serra Neto, Artur Marques, Sirlei G. Bomfim, Maria Rosa Q. Monteiro, Silvio G. de Souza, Rosangela C. Nunes, Rodolfo A. Microorganisms Article Antisepsis of the hands of medical personnel is one of the most important steps in the process of patient care, since direct contact can cause the cross-transfer of potentially pathogenic microorganisms at surgical sites. This study aimed to analyze the prevalence of microorganisms on the hands of 131 surgeons in a university hospital before the surgical procedure. Swabs were collected from each clinician’s hands before and after handwashing. The samples were placed in a transport medium and immediately delivered to a private clinical analysis laboratory from São Luis-Maranhão. The microorganisms were identified by ionization source mass spectrometry and matrix-assisted laser desorption (MALDI-TOF), and antibiotic susceptibility tests (AST) were performed using the Vitek2 and Phoenix-BD automated system. The results showed a high frequency (100%) of microorganisms before handwashing, but after surgical antisepsis, the rate dropped significantly (p < 0.05) to 27.5%. The gram-positive species most detected were Staphylococcus spp. and Micrococcus luteus, representing 83.9%, followed by gram-negative species, Stenotrophomonas maltophilia, Acinetobacter baumanii, Pseudomonas aeruginosa, Pseudomonas gessardi, Pantoea septica, Serratia marcescens, and Burkholderia lata. The effectiveness of hand antisepsis was 72.5%, demonstrating that surgeons’ hands are an important source of microorganisms that can cause infections in hospitalized patients in different care settings. MDPI 2023-07-27 /pmc/articles/PMC10456775/ /pubmed/37630455 http://dx.doi.org/10.3390/microorganisms11081895 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Serra Neto, Artur
Marques, Sirlei G.
Bomfim, Maria Rosa Q.
Monteiro, Silvio G.
de Souza, Rosangela C.
Nunes, Rodolfo A.
Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title_full Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title_fullStr Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title_full_unstemmed Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title_short Microbiological Analysis of Surgeons’ Hands in a Public Hospital in São Luis, Maranhão State, Brazil: A Cross-Sectional Study
title_sort microbiological analysis of surgeons’ hands in a public hospital in são luis, maranhão state, brazil: a cross-sectional study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456775/
https://www.ncbi.nlm.nih.gov/pubmed/37630455
http://dx.doi.org/10.3390/microorganisms11081895
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