Effects of Ion Combinations and Their Concentrations on Denitrification Performance and Gene Expressions of an Aerobic Strain Marinobacter Hydrocarbonoclasticus RAD-2

Salinity is one of the most important factors affecting the nitrogen-removal efficiency of denitrifying bacteria. A series of different ion combinations and salinity gradients were carried out to clarify the effects of ion types and concentrations on nitrogen removal by halophilic aerobic denitrifying bacteria RAD-2. Nitrate concentrations, nitrite concentrations, TAN concentrations, and OD(600) were monitored to investigate their effects on denitrification in each group. The results showed that Na(+), K(+), and Cl(-) accelerated the denitrification process and improved nitrogen-removal efficiency at moderate additions, while Ca(2+) and Mg(2+) showed no significant effect. Na(+) was effective alone, while K(+) or Cl(-) needed to be combined with at least one of Na(+), K(+), or Cl(-) to achieve similar efficiency. The batch tests of salinity confirmed that the addition of a moderate concentration of NaCl/Na(2)SO(4) could effectively improve nitrogen-removal efficiency, while excessive salinity might hinder denitrification metabolism. In the salinity range of 5~40‰, a 5‰ dosage might be the most economical method for strain RAD-2. Real-time PCR experiments on 17 key nitrogen metabolism-related genes revealed that chloride was widely involved in the nitrogen and carbon metabolism of microorganisms by altering cell osmotic pressure and opening ion channel proteins, thereby affecting the efficiency of denitrification. The results of this study may contribute to a better understanding of the different roles of various ions in aerobic denitrification and highlight the importance of salinity control in highly salted wastewater treatment.