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The Expectation and Reality of the HepG2 Core Metabolic Profile
To represent the composition of small molecules circulating in HepG2 cells and the formation of the “core” of characteristic metabolites that often attract researchers’ attention, we conducted a meta-analysis of 56 datasets obtained through metabolomic profiling via mass spectrometry and NMR. We hig...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456947/ https://www.ncbi.nlm.nih.gov/pubmed/37623852 http://dx.doi.org/10.3390/metabo13080908 |
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author | Kiseleva, Olga I. Kurbatov, Ilya Y. Arzumanian, Viktoriia A. Ilgisonis, Ekaterina V. Zakharov, Svyatoslav V. Poverennaya, Ekaterina V. |
author_facet | Kiseleva, Olga I. Kurbatov, Ilya Y. Arzumanian, Viktoriia A. Ilgisonis, Ekaterina V. Zakharov, Svyatoslav V. Poverennaya, Ekaterina V. |
author_sort | Kiseleva, Olga I. |
collection | PubMed |
description | To represent the composition of small molecules circulating in HepG2 cells and the formation of the “core” of characteristic metabolites that often attract researchers’ attention, we conducted a meta-analysis of 56 datasets obtained through metabolomic profiling via mass spectrometry and NMR. We highlighted the 288 most commonly studied compounds of diverse chemical nature and analyzed metabolic processes involving these small molecules. Building a complete map of the metabolome of a cell, which encompasses the diversity of possible impacts on it, is a severe challenge for the scientific community, which is faced not only with natural limitations of experimental technologies, but also with the absence of transparent and widely accepted standards for processing and presenting the obtained metabolomic data. Formulating our research design, we aimed to reveal metabolites crucial to the Hepg2 cell line, regardless of all chemical and/or physical impact factors. Unfortunately, the existing paradigm of data policy leads to a streetlight effect. When analyzing and reporting only target metabolites of interest, the community ignores the changes in the metabolomic landscape that hide many molecular secrets. |
format | Online Article Text |
id | pubmed-10456947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104569472023-08-26 The Expectation and Reality of the HepG2 Core Metabolic Profile Kiseleva, Olga I. Kurbatov, Ilya Y. Arzumanian, Viktoriia A. Ilgisonis, Ekaterina V. Zakharov, Svyatoslav V. Poverennaya, Ekaterina V. Metabolites Review To represent the composition of small molecules circulating in HepG2 cells and the formation of the “core” of characteristic metabolites that often attract researchers’ attention, we conducted a meta-analysis of 56 datasets obtained through metabolomic profiling via mass spectrometry and NMR. We highlighted the 288 most commonly studied compounds of diverse chemical nature and analyzed metabolic processes involving these small molecules. Building a complete map of the metabolome of a cell, which encompasses the diversity of possible impacts on it, is a severe challenge for the scientific community, which is faced not only with natural limitations of experimental technologies, but also with the absence of transparent and widely accepted standards for processing and presenting the obtained metabolomic data. Formulating our research design, we aimed to reveal metabolites crucial to the Hepg2 cell line, regardless of all chemical and/or physical impact factors. Unfortunately, the existing paradigm of data policy leads to a streetlight effect. When analyzing and reporting only target metabolites of interest, the community ignores the changes in the metabolomic landscape that hide many molecular secrets. MDPI 2023-08-03 /pmc/articles/PMC10456947/ /pubmed/37623852 http://dx.doi.org/10.3390/metabo13080908 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kiseleva, Olga I. Kurbatov, Ilya Y. Arzumanian, Viktoriia A. Ilgisonis, Ekaterina V. Zakharov, Svyatoslav V. Poverennaya, Ekaterina V. The Expectation and Reality of the HepG2 Core Metabolic Profile |
title | The Expectation and Reality of the HepG2 Core Metabolic Profile |
title_full | The Expectation and Reality of the HepG2 Core Metabolic Profile |
title_fullStr | The Expectation and Reality of the HepG2 Core Metabolic Profile |
title_full_unstemmed | The Expectation and Reality of the HepG2 Core Metabolic Profile |
title_short | The Expectation and Reality of the HepG2 Core Metabolic Profile |
title_sort | expectation and reality of the hepg2 core metabolic profile |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456947/ https://www.ncbi.nlm.nih.gov/pubmed/37623852 http://dx.doi.org/10.3390/metabo13080908 |
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