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Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice
BACKGROUND: Malaria remains a major public health problem in the tropical and subtropical regions. This study aimed of investigating the antimalarial and antioxidant activities of ethanol extract of Lophira lanceolata stem bark. Methodology. The antimalarial activity was determined using the Peter 4...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457170/ https://www.ncbi.nlm.nih.gov/pubmed/37637978 http://dx.doi.org/10.1155/2023/9400650 |
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author | Azizi, Mounvera Abdel Nadia, Noumedem Anangmo Christelle Cedric, Yamssi Sidiki, Ngouyamsa Nsapkain Aboubakar Guy-Armand, Gamago Nkadeu Esther, Djeussi Doriane Sandra, Tientcheu Noutong Jemimah Kevin, Tako Djimefo Alex Payne, Vincent Khan |
author_facet | Azizi, Mounvera Abdel Nadia, Noumedem Anangmo Christelle Cedric, Yamssi Sidiki, Ngouyamsa Nsapkain Aboubakar Guy-Armand, Gamago Nkadeu Esther, Djeussi Doriane Sandra, Tientcheu Noutong Jemimah Kevin, Tako Djimefo Alex Payne, Vincent Khan |
author_sort | Azizi, Mounvera Abdel |
collection | PubMed |
description | BACKGROUND: Malaria remains a major public health problem in the tropical and subtropical regions. This study aimed of investigating the antimalarial and antioxidant activities of ethanol extract of Lophira lanceolata stem bark. Methodology. The antimalarial activity was determined using the Peter 4-days' suppressive and Rane's curative tests on Swiss albino: these mice were infected with 1 × 10(7) parasitized red blood cells. The percentage reduction of parasitemia was related to each test, and the liver homogenate was used to assay malondialdehyde, superoxide dismutase, nitrogen monoxide, catalase, and glutathione for the evaluation of oxidative stress. During the curative test, blood was collected for hematological parameters, alanine aminotransferase and aspartate aminotransferase to evaluate liver function. RESULT: The ethanol extract of L. lanceolata showed a dose-dependent suppressive activity with the highest suppression of 88.22% at 500 mg/kg. Suppression produced by the extract was not significantly higher than that of the reference drug with 96.1%. Similarly, the extract at doses 125, 250, and 500 mg/kg showed significant decreases (P < 0.05) in a dose-dependent manner during the curative test. The ethanol extract of L. lanceolata caused a reduction of tissue markers, such as hepatic oxidative stress, as it increased the enzymatic activity of antioxidant enzymes. CONCLUSION: The ethanol extract of L. lanceolata possesses both antimalarial and antioxidant activities. However, further in vivo toxicity tests are required to guarantee their safety. |
format | Online Article Text |
id | pubmed-10457170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-104571702023-08-26 Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice Azizi, Mounvera Abdel Nadia, Noumedem Anangmo Christelle Cedric, Yamssi Sidiki, Ngouyamsa Nsapkain Aboubakar Guy-Armand, Gamago Nkadeu Esther, Djeussi Doriane Sandra, Tientcheu Noutong Jemimah Kevin, Tako Djimefo Alex Payne, Vincent Khan J Parasitol Res Research Article BACKGROUND: Malaria remains a major public health problem in the tropical and subtropical regions. This study aimed of investigating the antimalarial and antioxidant activities of ethanol extract of Lophira lanceolata stem bark. Methodology. The antimalarial activity was determined using the Peter 4-days' suppressive and Rane's curative tests on Swiss albino: these mice were infected with 1 × 10(7) parasitized red blood cells. The percentage reduction of parasitemia was related to each test, and the liver homogenate was used to assay malondialdehyde, superoxide dismutase, nitrogen monoxide, catalase, and glutathione for the evaluation of oxidative stress. During the curative test, blood was collected for hematological parameters, alanine aminotransferase and aspartate aminotransferase to evaluate liver function. RESULT: The ethanol extract of L. lanceolata showed a dose-dependent suppressive activity with the highest suppression of 88.22% at 500 mg/kg. Suppression produced by the extract was not significantly higher than that of the reference drug with 96.1%. Similarly, the extract at doses 125, 250, and 500 mg/kg showed significant decreases (P < 0.05) in a dose-dependent manner during the curative test. The ethanol extract of L. lanceolata caused a reduction of tissue markers, such as hepatic oxidative stress, as it increased the enzymatic activity of antioxidant enzymes. CONCLUSION: The ethanol extract of L. lanceolata possesses both antimalarial and antioxidant activities. However, further in vivo toxicity tests are required to guarantee their safety. Hindawi 2023-08-18 /pmc/articles/PMC10457170/ /pubmed/37637978 http://dx.doi.org/10.1155/2023/9400650 Text en Copyright © 2023 Mounvera Abdel Azizi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Azizi, Mounvera Abdel Nadia, Noumedem Anangmo Christelle Cedric, Yamssi Sidiki, Ngouyamsa Nsapkain Aboubakar Guy-Armand, Gamago Nkadeu Esther, Djeussi Doriane Sandra, Tientcheu Noutong Jemimah Kevin, Tako Djimefo Alex Payne, Vincent Khan Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title | Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title_full | Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title_fullStr | Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title_full_unstemmed | Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title_short | Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino's Mice |
title_sort | antimalarial efficacy and antioxidant activity of lophira lanceolata stem bark ethanol extract using plasmodium berghei induced-malaria in swiss albino's mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457170/ https://www.ncbi.nlm.nih.gov/pubmed/37637978 http://dx.doi.org/10.1155/2023/9400650 |
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