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Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs

In a framework aimed at the recovery and enhancement of medicinal plants endemic to the territory of the Cilento and Vallo di Diano National Park, Lavandula austroapennina N.G. Passal., Tundis and Upson has aroused interest. An insight into the chemical composition of the corolla, calyx, leaf, stem,...

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Autores principales: Chianese, Annalisa, Gravina, Claudia, Morone, Maria Vittoria, Ambrosino, Annalisa, Formato, Marialuisa, Palma, Francesca, Foglia, Francesco, Nastri, Bianca Maria, Zannella, Carla, Esposito, Assunta, De Filippis, Anna, Piccolella, Simona, Galdiero, Massimiliano, Pacifico, Severina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457779/
https://www.ncbi.nlm.nih.gov/pubmed/37631991
http://dx.doi.org/10.3390/v15081648
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author Chianese, Annalisa
Gravina, Claudia
Morone, Maria Vittoria
Ambrosino, Annalisa
Formato, Marialuisa
Palma, Francesca
Foglia, Francesco
Nastri, Bianca Maria
Zannella, Carla
Esposito, Assunta
De Filippis, Anna
Piccolella, Simona
Galdiero, Massimiliano
Pacifico, Severina
author_facet Chianese, Annalisa
Gravina, Claudia
Morone, Maria Vittoria
Ambrosino, Annalisa
Formato, Marialuisa
Palma, Francesca
Foglia, Francesco
Nastri, Bianca Maria
Zannella, Carla
Esposito, Assunta
De Filippis, Anna
Piccolella, Simona
Galdiero, Massimiliano
Pacifico, Severina
author_sort Chianese, Annalisa
collection PubMed
description In a framework aimed at the recovery and enhancement of medicinal plants endemic to the territory of the Cilento and Vallo di Diano National Park, Lavandula austroapennina N.G. Passal., Tundis and Upson has aroused interest. An insight into the chemical composition of the corolla, calyx, leaf, stem, and root organs was carried out following ultrasound-assisted maceration in n-hexane. The obtained lipophilic extracts were explored using ultra-high-performance chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI-QqTOF-MS/MS). The extracts from the different organs varied in their relative content of fatty acids, ursanes, and oleanane-type triterpenes. In particular, the oleanolic acid content appeared to increase in the order of corolla < leaf < stem. An MTT assay was performed to verify the possible cytotoxicity of the organ extracts of L. austroapennina at a concentration ranging from 12.5 to 400 µg/mL on the Vero CCL-81 cell line. Antiviral activity against herpes simplex virus type 1 (HSV-1), alpha human coronavirus 229E (HCoV-229E), and poliovirus type 1 (PV-1) was evaluated via a plaque reduction assay in the same cellular model. All the extracts did not show cytotoxic effects after 2 and 24 h exposure times, and the antiviral efficacy was particularly important for the stem extract, capable of completely inhibiting the tested viruses at low doses. The antiviral activity in a non-enveloped virus PV-1 allowed the assertion that the extracts from the organs of L. austroapennina, and especially the stem extract, interfered directly with the viral envelope. This study underlines how much knowledge of a territory’s medicinal plant heritage is a harbinger of promising discoveries in the health field.
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spelling pubmed-104577792023-08-27 Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs Chianese, Annalisa Gravina, Claudia Morone, Maria Vittoria Ambrosino, Annalisa Formato, Marialuisa Palma, Francesca Foglia, Francesco Nastri, Bianca Maria Zannella, Carla Esposito, Assunta De Filippis, Anna Piccolella, Simona Galdiero, Massimiliano Pacifico, Severina Viruses Article In a framework aimed at the recovery and enhancement of medicinal plants endemic to the territory of the Cilento and Vallo di Diano National Park, Lavandula austroapennina N.G. Passal., Tundis and Upson has aroused interest. An insight into the chemical composition of the corolla, calyx, leaf, stem, and root organs was carried out following ultrasound-assisted maceration in n-hexane. The obtained lipophilic extracts were explored using ultra-high-performance chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI-QqTOF-MS/MS). The extracts from the different organs varied in their relative content of fatty acids, ursanes, and oleanane-type triterpenes. In particular, the oleanolic acid content appeared to increase in the order of corolla < leaf < stem. An MTT assay was performed to verify the possible cytotoxicity of the organ extracts of L. austroapennina at a concentration ranging from 12.5 to 400 µg/mL on the Vero CCL-81 cell line. Antiviral activity against herpes simplex virus type 1 (HSV-1), alpha human coronavirus 229E (HCoV-229E), and poliovirus type 1 (PV-1) was evaluated via a plaque reduction assay in the same cellular model. All the extracts did not show cytotoxic effects after 2 and 24 h exposure times, and the antiviral efficacy was particularly important for the stem extract, capable of completely inhibiting the tested viruses at low doses. The antiviral activity in a non-enveloped virus PV-1 allowed the assertion that the extracts from the organs of L. austroapennina, and especially the stem extract, interfered directly with the viral envelope. This study underlines how much knowledge of a territory’s medicinal plant heritage is a harbinger of promising discoveries in the health field. MDPI 2023-07-28 /pmc/articles/PMC10457779/ /pubmed/37631991 http://dx.doi.org/10.3390/v15081648 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chianese, Annalisa
Gravina, Claudia
Morone, Maria Vittoria
Ambrosino, Annalisa
Formato, Marialuisa
Palma, Francesca
Foglia, Francesco
Nastri, Bianca Maria
Zannella, Carla
Esposito, Assunta
De Filippis, Anna
Piccolella, Simona
Galdiero, Massimiliano
Pacifico, Severina
Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title_full Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title_fullStr Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title_full_unstemmed Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title_short Lavandula austroapennina: Assessment of the Antiviral Activity of Lipophilic Extracts from Its Organs
title_sort lavandula austroapennina: assessment of the antiviral activity of lipophilic extracts from its organs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457779/
https://www.ncbi.nlm.nih.gov/pubmed/37631991
http://dx.doi.org/10.3390/v15081648
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