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Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury

Muscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this s...

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Detalles Bibliográficos
Autores principales: Siu, Wing-Sum, Ma, Hui, Cheng, Wen, Shum, Wai-Ting, Leung, Ping-Chung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457816/
https://www.ncbi.nlm.nih.gov/pubmed/37631059
http://dx.doi.org/10.3390/ph16081144
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author Siu, Wing-Sum
Ma, Hui
Cheng, Wen
Shum, Wai-Ting
Leung, Ping-Chung
author_facet Siu, Wing-Sum
Ma, Hui
Cheng, Wen
Shum, Wai-Ting
Leung, Ping-Chung
author_sort Siu, Wing-Sum
collection PubMed
description Muscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this study, an in vitro assay was used to test the cytotoxicity of a topical TCM formula containing Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR). Then, a muscle contusion rat model was developed to investigate the in vivo effect and basic mechanisms underlying CDR on muscle regeneration. The in vitro assay illustrated that CDR was non-cytotoxic to immortalized rat myoblast culture and increased cell viability. Histological results demonstrated that the CDR treatment facilitated muscle repair by increasing the number of new muscle fibers and promoting muscle integrity. The CDR treatment also upregulated the expression of Pax7, MyoD and myogenin, as evidenced by an immunohistochemical study. A gene expression analysis indicated that the CDR treatment accelerated the regeneration and remodeling phases during muscle repair. This study demonstrated that topical CDR treatment was effective at facilitating muscle injury repair.
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spelling pubmed-104578162023-08-27 Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury Siu, Wing-Sum Ma, Hui Cheng, Wen Shum, Wai-Ting Leung, Ping-Chung Pharmaceuticals (Basel) Article Muscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this study, an in vitro assay was used to test the cytotoxicity of a topical TCM formula containing Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR). Then, a muscle contusion rat model was developed to investigate the in vivo effect and basic mechanisms underlying CDR on muscle regeneration. The in vitro assay illustrated that CDR was non-cytotoxic to immortalized rat myoblast culture and increased cell viability. Histological results demonstrated that the CDR treatment facilitated muscle repair by increasing the number of new muscle fibers and promoting muscle integrity. The CDR treatment also upregulated the expression of Pax7, MyoD and myogenin, as evidenced by an immunohistochemical study. A gene expression analysis indicated that the CDR treatment accelerated the regeneration and remodeling phases during muscle repair. This study demonstrated that topical CDR treatment was effective at facilitating muscle injury repair. MDPI 2023-08-12 /pmc/articles/PMC10457816/ /pubmed/37631059 http://dx.doi.org/10.3390/ph16081144 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siu, Wing-Sum
Ma, Hui
Cheng, Wen
Shum, Wai-Ting
Leung, Ping-Chung
Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title_full Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title_fullStr Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title_full_unstemmed Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title_short Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
title_sort traditional chinese medicine for topical treatment of skeletal muscle injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457816/
https://www.ncbi.nlm.nih.gov/pubmed/37631059
http://dx.doi.org/10.3390/ph16081144
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