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LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection

A keloid is a benign tumor manifested as abnormal fibroplasia on the surface of the skin. Curing keloids has become a major clinical challenge, and searching for new treatments and medications has become critical. In this study, we developed a LA67 liposome-loaded thermo-sensitive hydrogel (LA67-RL-...

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Autores principales: Wan, Hongshuang, Wang, Shuangqing, Li, Chuying, Zeng, Bowen, Wu, Hao, Liu, Chao, Chen, Liqing, Jin, Mingji, Huang, Wei, Zang, Yingda, Zhang, Dongming, Gao, Zhonggao, Jin, Zhehu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457819/
https://www.ncbi.nlm.nih.gov/pubmed/37631371
http://dx.doi.org/10.3390/pharmaceutics15082157
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author Wan, Hongshuang
Wang, Shuangqing
Li, Chuying
Zeng, Bowen
Wu, Hao
Liu, Chao
Chen, Liqing
Jin, Mingji
Huang, Wei
Zang, Yingda
Zhang, Dongming
Gao, Zhonggao
Jin, Zhehu
author_facet Wan, Hongshuang
Wang, Shuangqing
Li, Chuying
Zeng, Bowen
Wu, Hao
Liu, Chao
Chen, Liqing
Jin, Mingji
Huang, Wei
Zang, Yingda
Zhang, Dongming
Gao, Zhonggao
Jin, Zhehu
author_sort Wan, Hongshuang
collection PubMed
description A keloid is a benign tumor manifested as abnormal fibroplasia on the surface of the skin. Curing keloids has become a major clinical challenge, and searching for new treatments and medications has become critical. In this study, we developed a LA67 liposome-loaded thermo-sensitive hydrogel (LA67-RL-Gel) with active targeting for treating keloids via peritumoral injection and explored the anti-keloid mechanism. Firstly, Arg-Gly-Asp (RGD) peptide-modified liposomes (LA67-RL) loaded with LA67 were prepared with a particle size of 105.9 nm and a Zeta potential of −27.4 mV, and an encapsulation efficiency of 89.6 ± 3.7%. We then constructed a thermo-sensitive hydrogel loaded with LA67-RL by poloxamer 407 and 188. The formulation was optimized through the Box–Behnken design, where the impact of the proportion of the ingredients on the quality of the hydrogel was evaluated entirely. The optimal formulation was 20.7% P407 and 2.1% P188, and the gelation time at 37 °C was 9.5 s. LA67-RL-Gel slowly released 92.2 ± 0.8% of LA67 at pH 6.5 PBS for 72 h. LA67-RL-Gel increased adhesion with KF cells; increased uptake; promoted KF cells apoptosis; inhibited cell proliferation; reduced α-SMA content; decreased collagen I, collagen III, and fibronectin deposition; inhibited angiogenesis; and modulated the keloid microenvironment, ultimately exerting anti-keloid effects. In summary, this simple, low-cost, and highly effective anti-keloid liposome hydrogel provides a novel approach for treating keloids and deserves further development.
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spelling pubmed-104578192023-08-27 LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection Wan, Hongshuang Wang, Shuangqing Li, Chuying Zeng, Bowen Wu, Hao Liu, Chao Chen, Liqing Jin, Mingji Huang, Wei Zang, Yingda Zhang, Dongming Gao, Zhonggao Jin, Zhehu Pharmaceutics Article A keloid is a benign tumor manifested as abnormal fibroplasia on the surface of the skin. Curing keloids has become a major clinical challenge, and searching for new treatments and medications has become critical. In this study, we developed a LA67 liposome-loaded thermo-sensitive hydrogel (LA67-RL-Gel) with active targeting for treating keloids via peritumoral injection and explored the anti-keloid mechanism. Firstly, Arg-Gly-Asp (RGD) peptide-modified liposomes (LA67-RL) loaded with LA67 were prepared with a particle size of 105.9 nm and a Zeta potential of −27.4 mV, and an encapsulation efficiency of 89.6 ± 3.7%. We then constructed a thermo-sensitive hydrogel loaded with LA67-RL by poloxamer 407 and 188. The formulation was optimized through the Box–Behnken design, where the impact of the proportion of the ingredients on the quality of the hydrogel was evaluated entirely. The optimal formulation was 20.7% P407 and 2.1% P188, and the gelation time at 37 °C was 9.5 s. LA67-RL-Gel slowly released 92.2 ± 0.8% of LA67 at pH 6.5 PBS for 72 h. LA67-RL-Gel increased adhesion with KF cells; increased uptake; promoted KF cells apoptosis; inhibited cell proliferation; reduced α-SMA content; decreased collagen I, collagen III, and fibronectin deposition; inhibited angiogenesis; and modulated the keloid microenvironment, ultimately exerting anti-keloid effects. In summary, this simple, low-cost, and highly effective anti-keloid liposome hydrogel provides a novel approach for treating keloids and deserves further development. MDPI 2023-08-18 /pmc/articles/PMC10457819/ /pubmed/37631371 http://dx.doi.org/10.3390/pharmaceutics15082157 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wan, Hongshuang
Wang, Shuangqing
Li, Chuying
Zeng, Bowen
Wu, Hao
Liu, Chao
Chen, Liqing
Jin, Mingji
Huang, Wei
Zang, Yingda
Zhang, Dongming
Gao, Zhonggao
Jin, Zhehu
LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title_full LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title_fullStr LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title_full_unstemmed LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title_short LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
title_sort la67 liposome-loaded thermo-sensitive hydrogel with active targeting for efficient treatment of keloid via peritumoral injection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457819/
https://www.ncbi.nlm.nih.gov/pubmed/37631371
http://dx.doi.org/10.3390/pharmaceutics15082157
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