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Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study

Dielectrophoresis (DEP) is one of the microfluid-based techniques that can manipulate the red blood cells (RBC) for blood plasma separation, which is used in many medical screening/diagnosis applications. The tapered aluminium microelectrode array (TAMA) is fabricated for potential sensitivity enhan...

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Autores principales: Samad, Muhammad Izzuddin Abd, Ponnuthurai, Darven Raj, Badrudin, Syazwani Izrah, Ali, Mohd Anuar Mohd, Razak, Mohd Azhar Abdul, Buyong, Muhamad Ramdzan, Latif, Rhonira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457829/
https://www.ncbi.nlm.nih.gov/pubmed/37630162
http://dx.doi.org/10.3390/mi14081625
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author Samad, Muhammad Izzuddin Abd
Ponnuthurai, Darven Raj
Badrudin, Syazwani Izrah
Ali, Mohd Anuar Mohd
Razak, Mohd Azhar Abdul
Buyong, Muhamad Ramdzan
Latif, Rhonira
author_facet Samad, Muhammad Izzuddin Abd
Ponnuthurai, Darven Raj
Badrudin, Syazwani Izrah
Ali, Mohd Anuar Mohd
Razak, Mohd Azhar Abdul
Buyong, Muhamad Ramdzan
Latif, Rhonira
author_sort Samad, Muhammad Izzuddin Abd
collection PubMed
description Dielectrophoresis (DEP) is one of the microfluid-based techniques that can manipulate the red blood cells (RBC) for blood plasma separation, which is used in many medical screening/diagnosis applications. The tapered aluminium microelectrode array (TAMA) is fabricated for potential sensitivity enhancement of RBC manipulation in lateral and vertical directions. In this paper, the migration properties of dielectrophoretically manipulated RBC in TAMA platform are studied at different peak-to-peak voltage (Vpp) and duration supplied onto the microelectrodes. Positive DEP manipulation is conducted at 440 kHz with the RBC of 4.00 ± 0.2 µm average radius attracted to the higher electric field intensity regions, which are the microelectrodes. High percentage of RBC migration occurred at longer manipulation time and high electrode voltage. During DEP manipulation, the RBC are postulated to levitate upwards, experience the electro-orientation mechanism and form the pearl chains before migrating to the electrodes. The presence of external forces other than the dielectrophoretic force may also affect the migration response of RBC. The safe operating limit of 10 Vpp and manipulation duration of ≤50 s prevent RBC rupture while providing high migration percentage. It is crucial to define the safe working region for TAMA devices that manipulate small RBC volume (~10 µL).
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spelling pubmed-104578292023-08-27 Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study Samad, Muhammad Izzuddin Abd Ponnuthurai, Darven Raj Badrudin, Syazwani Izrah Ali, Mohd Anuar Mohd Razak, Mohd Azhar Abdul Buyong, Muhamad Ramdzan Latif, Rhonira Micromachines (Basel) Article Dielectrophoresis (DEP) is one of the microfluid-based techniques that can manipulate the red blood cells (RBC) for blood plasma separation, which is used in many medical screening/diagnosis applications. The tapered aluminium microelectrode array (TAMA) is fabricated for potential sensitivity enhancement of RBC manipulation in lateral and vertical directions. In this paper, the migration properties of dielectrophoretically manipulated RBC in TAMA platform are studied at different peak-to-peak voltage (Vpp) and duration supplied onto the microelectrodes. Positive DEP manipulation is conducted at 440 kHz with the RBC of 4.00 ± 0.2 µm average radius attracted to the higher electric field intensity regions, which are the microelectrodes. High percentage of RBC migration occurred at longer manipulation time and high electrode voltage. During DEP manipulation, the RBC are postulated to levitate upwards, experience the electro-orientation mechanism and form the pearl chains before migrating to the electrodes. The presence of external forces other than the dielectrophoretic force may also affect the migration response of RBC. The safe operating limit of 10 Vpp and manipulation duration of ≤50 s prevent RBC rupture while providing high migration percentage. It is crucial to define the safe working region for TAMA devices that manipulate small RBC volume (~10 µL). MDPI 2023-08-17 /pmc/articles/PMC10457829/ /pubmed/37630162 http://dx.doi.org/10.3390/mi14081625 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Samad, Muhammad Izzuddin Abd
Ponnuthurai, Darven Raj
Badrudin, Syazwani Izrah
Ali, Mohd Anuar Mohd
Razak, Mohd Azhar Abdul
Buyong, Muhamad Ramdzan
Latif, Rhonira
Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title_full Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title_fullStr Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title_full_unstemmed Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title_short Migration Study of Dielectrophoretically Manipulated Red Blood Cells in Tapered Aluminium Microelectrode Array: A Pilot Study
title_sort migration study of dielectrophoretically manipulated red blood cells in tapered aluminium microelectrode array: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457829/
https://www.ncbi.nlm.nih.gov/pubmed/37630162
http://dx.doi.org/10.3390/mi14081625
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