Cargando…
Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses
Kelussia odoratissima Mozaff. is a species of Apiaceae endemic to the Zagros Mountains in Iran. In the present investigation, for the first time, the polyphenolic compounds and flavonoids of its leaves were determined by liquid chromatography-mass spectrometry (LC-MS). As a result, p-coumaric acid,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457879/ https://www.ncbi.nlm.nih.gov/pubmed/37630391 http://dx.doi.org/10.3390/molecules28166140 |
_version_ | 1785097030443466752 |
---|---|
author | Rahimmalek, Mehdi Szumny, Antoni Gharibi, Shima Pachura, Natalia Miroliaei, Mehran Łyczko, Jacek |
author_facet | Rahimmalek, Mehdi Szumny, Antoni Gharibi, Shima Pachura, Natalia Miroliaei, Mehran Łyczko, Jacek |
author_sort | Rahimmalek, Mehdi |
collection | PubMed |
description | Kelussia odoratissima Mozaff. is a species of Apiaceae endemic to the Zagros Mountains in Iran. In the present investigation, for the first time, the polyphenolic compounds and flavonoids of its leaves were determined by liquid chromatography-mass spectrometry (LC-MS). As a result, p-coumaric acid, ferulic acid, caffeic acid, chlorogenic acid, acetyl phloroglucinol, vanillic acid, m-coumaric acid, and 4-methylsiringol were determined as the main phenolic compounds, while 3-hydroxyflavone, flavone, quercetin, rutin, neohesperidin, polydatin, and diosmin were the main flavonoid components, of which chlorogenic acid (303.08 µL/gDW), neohesperidin (38.37 µL/gDw), and diosmin (28.62 µL/gDW) were the most abundant. Solid-phase microextraction (SPME) was also used to determine the chemical compounds. Based on SPME, (Z)-undec-6-en-2-one (17.48%) and (Z)-butylidenephthalide (4.348%) were the major components. Based on GC-MS analyses, (Z)-ligustilide was the main compound; however, some new compounds were also determined, including 3-ethylisobenzofuran-1 (3H)-one, (E)-ligugustilide, and E-n-butylidene phthalide. Also, for the first time, we have identified EOs ethyl and isobutyl phthalides on the basis of the obtained EI-MS spectra. Finally, the fragmentation of phthalides is also discussed in this research. |
format | Online Article Text |
id | pubmed-10457879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104578792023-08-27 Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses Rahimmalek, Mehdi Szumny, Antoni Gharibi, Shima Pachura, Natalia Miroliaei, Mehran Łyczko, Jacek Molecules Article Kelussia odoratissima Mozaff. is a species of Apiaceae endemic to the Zagros Mountains in Iran. In the present investigation, for the first time, the polyphenolic compounds and flavonoids of its leaves were determined by liquid chromatography-mass spectrometry (LC-MS). As a result, p-coumaric acid, ferulic acid, caffeic acid, chlorogenic acid, acetyl phloroglucinol, vanillic acid, m-coumaric acid, and 4-methylsiringol were determined as the main phenolic compounds, while 3-hydroxyflavone, flavone, quercetin, rutin, neohesperidin, polydatin, and diosmin were the main flavonoid components, of which chlorogenic acid (303.08 µL/gDW), neohesperidin (38.37 µL/gDw), and diosmin (28.62 µL/gDW) were the most abundant. Solid-phase microextraction (SPME) was also used to determine the chemical compounds. Based on SPME, (Z)-undec-6-en-2-one (17.48%) and (Z)-butylidenephthalide (4.348%) were the major components. Based on GC-MS analyses, (Z)-ligustilide was the main compound; however, some new compounds were also determined, including 3-ethylisobenzofuran-1 (3H)-one, (E)-ligugustilide, and E-n-butylidene phthalide. Also, for the first time, we have identified EOs ethyl and isobutyl phthalides on the basis of the obtained EI-MS spectra. Finally, the fragmentation of phthalides is also discussed in this research. MDPI 2023-08-19 /pmc/articles/PMC10457879/ /pubmed/37630391 http://dx.doi.org/10.3390/molecules28166140 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rahimmalek, Mehdi Szumny, Antoni Gharibi, Shima Pachura, Natalia Miroliaei, Mehran Łyczko, Jacek Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title | Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title_full | Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title_fullStr | Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title_full_unstemmed | Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title_short | Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses |
title_sort | chemical investigations in kelussia odoratissima mozaff. leaves based on comprehensive analytical methods: lc-ms, spme, and gc-ms analyses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457879/ https://www.ncbi.nlm.nih.gov/pubmed/37630391 http://dx.doi.org/10.3390/molecules28166140 |
work_keys_str_mv | AT rahimmalekmehdi chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses AT szumnyantoni chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses AT gharibishima chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses AT pachuranatalia chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses AT miroliaeimehran chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses AT łyczkojacek chemicalinvestigationsinkelussiaodoratissimamozaffleavesbasedoncomprehensiveanalyticalmethodslcmsspmeandgcmsanalyses |